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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 756 results for: ELP

Showing: 201 - 220 of 756 results

  1. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...obvious, in that a lot of science communication is helping people understand new concepts. You hopefully are... your audience knows, but the following tips can help:  If there’s a teacher, mentor, or other educator...target audience. Thinking about cognitive load will help you understand why you’re not communicating as clearly...

  2. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...The Rare Genomics Institute BeHEARD (Helping Empower and Accelerate Research Discoveries) Challenge provides...SLC6A8 deficiency. “This proof of concept study will help in identification of new targets that could greatly...release in keratinocytes,” says Mellett. This would help the lab identify therapeutic targets in the future...

  3. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...score ~99.9% helpful and the customer and technical service teams put 100% effort into helping our customers...internal referrals. Our long reaching reputation helps us hire great people from farther away via connections...

  4. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...Clarify: Asking for clarification during a meeting can help get everyone on the same page. Others in the meeting...might also have the same question and asking can help the team identify possible points of confusion in...measures in improving cross team communication has helped Addgene move from completing 2-3 large projects...

  5. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...nifty technique, but what kinds of questions can it help answer? Ramanathan et al. outline three applications...breast cancer. Infectious Disease. RaPID can also help identify which host proteins interact with viral...interactions. Do you have a question that RaPID could help answer? The RaPID plasmids are available from Addgene...

  6. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...over the years with the hope that our insights can help steer you in the right direction when writing your...examples are intentionally trivial, but they're here to help tell this story. In this first example to the right...Addgene and is interested in writing software tools to help scientists. You can find him on Twitter @krisshamloo...

  7. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...symposium we will discuss where better data is needed to help shape and inform the overall structure of the biomedical...negative, of changing the workforce structure? Helping to answer some of these questions, and posing some...particular about postdocs. Among other involvements, he helped to establish grants through the Sloan foundation...

  8. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...assembly design (DAD) to build out three tools to help researchers design high-fidelity Golden Gate Assemblies...accomplished with Golden Gate Assembly—even when getting help from DAD. The 35-piece assembly mentioned above ...deposited by NEB – and don’t forget to ask DAD for some help! References Potapov, V., Ong, J. L., Kucera, R...

  9. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...deficient in E1, E2, E3, and E4. Gutless Ad vectors (helper-dependent (HD-Ad), or high-capacity (HC-Ad__ are...packaging signal in cis. Therefore, co-infection with a helper adenovirus is required to provide the viral proteins... production is more complex and the presence of helper virus contaminants have slowed the use of this ...

  10. Lab Automation at Addgene

    Type
    Blog Post
    ...modified organisms. Since Addgene is very proud to help scientists share plasmids across the globe, we’re...generate far more legible labels than your hand and help keep things more organized. Cap tubes with a micronic... it isn’t electronic but it is one of the most helpful machines I have used in the lab. It’s especially...

  11. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...only a great form of scientific outreach, it also helps you to hone your writing skills and make a name ...Science Writers Association, EUSJA or ABSW for helpful tips and job offers. If you live in the UK, join...taking a media or journalism course. Courses can help you gain work experience and build your network,...

  12. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...interest — for example, AAV8 for kidney cells — can help improve infection rates in your experiment. ... screen to a high degree. This is particularly helpful when investigating tissue-specific diseases, as...over lentivirus in many mouse tissues. This will help improve your selection pool for downstream NGS and...

  13. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...decrease the compensation value. If neither of these helps shift your population into the correct spot, you...markers can be challenging! But we hope this guide can help you get started. Remember, you can always change...finalize your compensation values later. This can be helpful as you're learning! Ashlyn has a research background...

  14. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...Where is the CRISPR community?  Addgene exists to help researchers share useful tools, and as such, we’...challenging. Addgene’s free educational resources help scientists navigate that learning curve. As of 2023...Genome Edit has 76,011 views.  Has all of this work helped scientists use CRISPR technology? We think so —...

  15. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...website for any relevant hashtags and handles to help you find other scientists attending. Reach out if...the conference hashtag to set one up. It’s also helpful to write your Twitter handle on your nametag for...are not looking for a job - those connections can help you later on. Update your LinkedIn and your resume...

  16. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ... bullet points for what you would like to say to help build your presentation. For example, when thinking... fundamental takeaway. Writing these points will help you remember what to say and provide your audience...Having them reframe or elaborate their thought will help you to provide an on-topic answer by creating time...

  17. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...addition to human error, fragments of DNA from the helper plasmids or the cell genome can be packaged inside...genome, bacterial genomes, cloning vectors, and helper plasmids (Chadeuf et al., 2005, Wright et al., ...to discuss. We are also available by phone or at help@addgene.org to answer your questions about our quality...

  18. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ... to submit an updated protocol? Just email us at help@addgene.org and we will be happy to upload your ...every lab's needs. Plus there are the apps - many to help you access your protocols at the bench, keep your...protocols show up on the related plasmid pages and can help scientists use these plasmids more successfully....

  19. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...Alignment Search Tool (BLAST) provided by NCBI. BLAST helps us compare the sequencing results of the plasmids...differences in the blastn programs, check out the BLAST Help webpage. Optimizing blastx searches Once you have... on our suggestions? Share your thoughts here to help other labs speed up their plasmid and cloning verification...

  20. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...recurring meeting amongst scientists that aims to help stay current on new and emerging work by collaboratively...unable to attend for scheduling conflicts. This helped us find a time that accommodates more people. The...(multiple choice/binary) and open questions is helpful to ensure acquisition of the essential data and...
Showing: 201 - 220 of 756 results