...plasmids and to receive important coronavirus-related deposits. Our valiant lab and shipping staff agreed to ...to come in and start getting plasmid deposits into the collection and to start shipping. They created the...also moved fast to ensure that the majority of deposited materials to study SARS-CoV-2 would be fully open...

... development of several fluorescent autophagy reporters now allows for the measurement of autophagic flux... al. "Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...using a single fluorescent biosensor." Scientific reports 5 (2015): 12397. PubMed PMID: 26215030. PubMed ...

... cells and activate expression of a luciferase reporter. CPP was not required for protein entry into cells...primary cell types using Cre and various loxP reporters. In both mouse and human embryonic stem cells,...Plasmids @Addgene: iTOP-tested proteins and reporters Previous Protein Delivery Techniques: Introduction...

...specific author guidelines for reporting your materials. If you deposit a plasmid with Addgene, your plasmid...to ensure that materials available through our repository are verified and identifiable to save researchers...

...actively require these skills, including: Science Reporter Scientific Sales Epidemiologist Scientific Recruitment... actively use these skills include: Science Reporter Scientific Illustrator Research and Development...desirable in a range of roles, including: Science Reporter Technical Writer Statistician Bioinformatician...

...control process alone takes nearly a year. Addgene depositor Juan Carlos Izpsua Belmonte said it took the joint...the scientists I've already mentioned, Addgene depositors Marius Wernig, Kristin Baldwin, Kathrin Plath...spoken at this meeting. In fact, we have so many depositors from the stem cell community that they have their...

...not used for general plasmid selection. Instead, reporter genes are often employed to easily monitor transfection...expression levels in the cells. Ideally, the chosen reporter is unique to the cell, is expressed from the plasmid...assess transfection success. GFP is often used as a reporter and we will be covering this ever-versatile fluorophore...

...Biotechnologies' press release about their Cas13d deposit here). Konermann et al.: CasRx is a highly potent...localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx and AdmCas13d produced 92% and 87%...continue to grow, and we at Addgene appreciate our depositors for making their tools available to the broader...

...entire mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows...and cited figures from papers or image sharing repositories. Then we format, do a final review to catch ...informative resources that answer questions our depositors and customers are asking. So how can you get ...

...promoter, thereby activating transcription. Addgene depositors have developed heat shock-inducible Cre and Cas9... phage promoter pR, preventing expression of a reporter gene. When light is present, YFI is inactive, ...preventing repressor cI synthesis and allowing reporter gene transcription to take place. Addgene also...

...all available sequence information from Addgene depositors and our own sequencing results: On this page ...sequence in your sequence viewer of choice. If the depositor provided a custom annotated GenBank file, this...updates will make it much easier to browse the repository and quickly identify the plasmids and features...

...usually spread unidirectionally, there are some reports of bi-directionality. For predominately anterograde...cleft is usually severely impaired, and only few reports exist about dye molecules that are transported ...2008; Watakabe et al., 2015). There are several reports of both retrograde and anterograde intracellular...replication like RABV or HSV. There are, however, a few reports of trans-synaptic spreading of some AAVs, specifically...

...by reducing the distance between the target and reporter. They have fewer non-specific binding opportunities...it either needs to be directly conjugated to a reporter or you need a light-chain specific conjugated ...fairly easy to find these in a Fab fragment format. Reporter-conjugated anti-IgG Fab fragments make good secondaries...

...do? Let’s take a look at how plasmids recently deposited by Chris Voigt’s lab help keep gene expression...buffered against the effects of copy number on reporter gene expression. On the other hand, using a constitutive...Download the Plasmid 101 eBook See all plasmids deposited by the Voigt lab ...

... an ERK kinase translocation reporter with a mRuby2 fluorescent reporter (ERK KTR-mRuby2). The ERK KTR...Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable fluorescent proteins...

...pH-sensitive fluorescent biosensor that was recently deposited with Addgene by Dr. Mark Prescott. This system...2008). Rosella: A fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...using a single fluorescent biosensor. Scientific Reports,5(1). doi:10.1038/srep12397. PubMed PMID: 26215030...

...community. This is no small task, however, at a repository that consistently receives around 200 new DNA...data on every plasmid we distribute through our deposit process, and then we carefully track samples through...your effort. In addition, if you should choose to deposit your plasmids with us, it will be easy to find ...

...researcher, Carrie Bhang. The ClonTracer Library, deposited by Carrie Bhang, a research investigator in the...to EGFR inhibitor (Hata et al., 2016).  Bhang deposited the library with Addgene because she wanted to...sharing protocols one by one,” and she is excited to deposit the library with Addgene because it is a “really...

...vector blog posts for updates on the most recent deposits, protocols, and tips for using retroviral vectors...Plasmid #17408)   This is a lentiviral vector deposited by Dr. Tyler Jacks. It carries CRE recombinase...together with expression of fluorescent or enzymatic reporters for cellular assays and animal imaging studies...

...PMM2 gene. Videira’s group recently reviewed and reported that PMM2-CDG patients suffer from immunological...drugs can be used to treat these diseases (drug repositioning). The ultimate goal of this work is to lower... construct NFIX expression vectors as well as reporters of NFIX function. They will then mutagenize the...