We narrowed to 657 results for: EPO

...bacteria, but have no fear — plasmids from Addgene depositors are making it easier than ever to use CRISPR ...double stranded breaks (DSB) are lethal. Addgene depositor Luciano Marraffini’s lab took advantage of this...challenging problem than repression, scientists have deposited several plasmid tools that can be used in this...microbiome Beyond the human gut, the Barrick lab has deposited plasmids that can be used to engineer the bee ...

...Thanks to five different labs across the U.S. who deposited their plasmids with Addgene to share with the ...borders. This past year, we welcomed over 500 new depositing labs who entrusted us with their research materials... global scientific community. Whether you're depositing plasmids, ordering viral vectors, or exploring...

...viral infection a) antibody-dependent complement deposition (ADCD) b) antibody-dependent cellular cytotoxicity...., 2019.    Antibody-dependent complement deposition (ADCD) Antibodies can bind to a pathogen and ...opsonization. In antibody-dependent complement deposition (ADCD), opsonization of IgG and IgM antibodies...

...positive or more negative.  Neuronal stimulation: Depolarization When the inside of the neuron becomes positive...positive over a certain threshold, it becomes depolarized and will fire a signal. The non-specific cation...both cause ion movement that results in cell depolarization. They therefore function as a neuron “on switch...

... the most frequently requested vectors in our repository and will discuss some of the features you may...Some of the more popular studies involve using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. ...animals, gene targeting and genome modifications to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g...

...plasmids and to receive important coronavirus-related deposits. Our valiant lab and shipping staff agreed to ...to come in and start getting plasmid deposits into the collection and to start shipping. They created the...also moved fast to ensure that the majority of deposited materials to study SARS-CoV-2 would be fully open...

...the DNA-binding domain of AraC using a mCherry reporter downstream of a PBAD promoter. While none of the...contains BLADE, the PBAD promoter, and a mCherry reporter downstream of the PBAD promoter. To use this plasmid...shared with Addgene. You can find all the plasmids deposited from Di Ventura's lab, including the popular light-inducible...

... development of several fluorescent autophagy reporters now allows for the measurement of autophagic flux... al. "Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...using a single fluorescent biosensor." Scientific reports 5 (2015): 12397. PubMed PMID: 26215030. PubMed ...

... cells and activate expression of a luciferase reporter. CPP was not required for protein entry into cells...primary cell types using Cre and various loxP reporters. In both mouse and human embryonic stem cells,...Plasmids @Addgene: iTOP-tested proteins and reporters Previous Protein Delivery Techniques: Introduction...

...actively require these skills, including: Science Reporter Scientific Sales Epidemiologist Scientific Recruitment... actively use these skills include: Science Reporter Scientific Illustrator Research and Development...desirable in a range of roles, including: Science Reporter Technical Writer Statistician Bioinformatician...

...specific author guidelines for reporting your materials. If you deposit a plasmid with Addgene, your plasmid...to ensure that materials available through our repository are verified and identifiable to save researchers...

...control process alone takes nearly a year. Addgene depositor Juan Carlos Izpsua Belmonte said it took the joint...the scientists I've already mentioned, Addgene depositors Marius Wernig, Kristin Baldwin, Kathrin Plath...spoken at this meeting. In fact, we have so many depositors from the stem cell community that they have their...

...not used for general plasmid selection. Instead, reporter genes are often employed to easily monitor transfection...expression levels in the cells. Ideally, the chosen reporter is unique to the cell, is expressed from the plasmid...assess transfection success. GFP is often used as a reporter and we will be covering this ever-versatile fluorophore...

...usually spread unidirectionally, there are some reports of bi-directionality. For predominately anterograde...cleft is usually severely impaired, and only few reports exist about dye molecules that are transported ...2008; Watakabe et al., 2015). There are several reports of both retrograde and anterograde intracellular...replication like RABV or HSV. There are, however, a few reports of trans-synaptic spreading of some AAVs, specifically...

...all available sequence information from Addgene depositors and our own sequencing results: On this page ...sequence in your sequence viewer of choice. If the depositor provided a custom annotated GenBank file, this...updates will make it much easier to browse the repository and quickly identify the plasmids and features...

...Biotechnologies' press release about their Cas13d deposit here). Konermann et al.: CasRx is a highly potent...localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx and AdmCas13d produced 92% and 87%...continue to grow, and we at Addgene appreciate our depositors for making their tools available to the broader...

...promoter, thereby activating transcription. Addgene depositors have developed heat shock-inducible Cre and Cas9... phage promoter pR, preventing expression of a reporter gene. When light is present, YFI is inactive, ...preventing repressor cI synthesis and allowing reporter gene transcription to take place. Addgene also...

...entire mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows...and cited figures from papers or image sharing repositories. Then we format, do a final review to catch ...informative resources that answer questions our depositors and customers are asking. So how can you get ...

...by reducing the distance between the target and reporter. They have fewer non-specific binding opportunities...it either needs to be directly conjugated to a reporter or you need a light-chain specific conjugated ...fairly easy to find these in a Fab fragment format. Reporter-conjugated anti-IgG Fab fragments make good secondaries...

... an ERK kinase translocation reporter with a mRuby2 fluorescent reporter (ERK KTR-mRuby2). The ERK KTR...Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable fluorescent proteins...