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We narrowed to 527 results for: HA tag

Showing: 201 - 220 of 527 results

  1. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...to allow in-frame deletions and introduce epitope tags and RNA transcription start sites. The lab further...packaging, but their activity in mammalian systems has been in question. Three different labs have recently...CRISPR-Cas system for mammalian use Stanley Qi’s lab has developed a miniature Cas system (CasMINI) engineered...

  2. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...FPs) offer scientists a simple yet powerful way to tag cellular proteins and investigate protein localization...fusion, etc). For example, by engineering PA-GFP tagged to a mitochondrial matrix protein (mito-PAGFP) ...Markus, and Wei‐Lih Lee. "Photoactivatable GFP tagging cassettes for protein‐tracking studies in the budding...high contrast ratio usually indicates that a PA-FP has a low level of spontaneous fluorescence in the absence...an FP-chimeric protein may be at any one of many stages of synthesis and degradation. For this reason it...PA-FPs by performing several rounds of random mutagenesis screening for enhanced RFP variants. PA-mCherry...negative” photoswitching. In 2008, scientists used mutagenesis screens to find a version of Dronpa that doesn't...

  3. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...until the pups are 2-4 weeks old and use the ear tag method (creating different patterns of ear holes ...Samantha Young. The use of CRISPR/Cas9 for gene editing has expanded since its adaptation for use in mammalian...biology surrounding a particular gene (although it has been quite successfully adapted for use in screening... method for validating your selected gRNA’s, and has the bonus benefit of supplying you with primers for...sturdier than the delicate RNA and therefore there has less chance of contamination with RNase leading to...products will give specific details on what exactly has been inserted or deleted. Each lab will use a slightly... et al. "Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes....

  4. Plan Your Experiment

    Type
    Collection
    ...specific genomic edits, like point mutations or small tag insertions, by copying from a DNA donor template ...contain your desired edit. Each of these methods has their own considerations. Which one to use will come...lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. Both lentiviral and... if you provided a homology template, a large percentage of DNA breaks will be repaired by NHEJ instead...

  5. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ... applications (v82 has higher affinity for glutamate but slower kinetics; v857 has faster kinetics but...but lower affinity) and with a variety of tags for localization (PDGFR, GPI, and SGZ). Read more from the...iGluSnFR3 (Aggarwal et al. 2022). This new version has higher signal to noise ratio and is better localized...for two-photon microscopy. The resulting JEDI-2P has faster response kinetics and increased brightness...and deep-tissue imaging. Vladislav Verkhusha’s lab has generated a new genetically encoded calcium indicator...interest, it’s important to choose a biosensor that has a binding affinity close to the range of concentrations...physiology and metabolism under various conditions. LiLac has improved response size and reduced sensitivity to...

  6. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...epitope-targeting antibody that is conjugated to a biotin tag but not a fluorophore. In a separate step, cells ...interest. Indirect labeling is achieved through protein-tag or protein-protein interactions, like biotin-streptavidin...selecting the primary antibody host species, as it has to be different from the other antibodies in your...target marker, allowing for its detection. The advantage of this system is that streptavidin conjugates...the primary antibody (Figure 1b). As above, the advantage of this system is increased flexibility for panel...

  7. MXS Chaining

    Type
    Blog Post
    ...localization 1 TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag (derived from...the newly generated MCS. MXS-chaining also has the advantage of being directional, meaning that (in the... cloning. MXS Chaining is one such technique and has been used to produce complex fluorescent reporter...culture systems (their original goal). Their work has demonstrated that the MXS-chaining method can be ...

  8. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...Post About Yeast Vectors Read our Blog Post About Tagging Yeast Genes ...cells where binding between the proteins of interest has occurred. Although the original Y2H systems utilized... number of different bait and prey vectors. This has been shown to be as effective as using five independent...transmembrane proteins, a split ubiquitin system has been devised (8). References 1. Casadaban M.J.,...activator (1a) or a repressor (1b). Scientists took advantage of these discoveries to create a new system for...subset based on a cellular compartment or growth stage). A prey library can either be applied to the bait...

  9. Five Popular Model Organisms

    Type
    Blog Post
    ...the the developing zebrafish pancreas. They first tagged these cells using plasmids. Then using HOTcre, ...more commonly known: the fruit fly. The fruit fly has been used in scientific research for over a century... a transgenic mouse line. Over the years the fly has become an ideal model organism to study an array ...development, genetics, and the nervous system. The fly has also emerged as an important human disease model ... described in 1993 by Norbert Perrimon’s lab and has been continuously improved upon since then. If you...Zebrafish (Danio rerio) Since the 1960s, the zebrafish has gradually become an important model organism. They...Caenorhabditis elegans) The worm, Caenorhabditis elegans, has been used extensively as a model organism since the...

  10. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...received a whole set of cards (Promoters, Inserts, Tags, Origins of Replication, Resistance gene and Selection... As I wrote in the blog post, BUILD YOUR PLASMID has been developed to teach gamma-retrovirus or lentivirus-based...

  11. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...any real traction.  This can carry a heavy price tag, especially if you're working on something other ...this deter your ambition. If you believe your idea has potential to make a difference, then make sure you...Boston-based startup focused on empowering early-stage scientists. You can think of miLab as a sort of ...biotechnology investment fund that would apply a percentage of returns to support student projects, but it...for such a fund is an impossibility at our early stage.  After each pitch we made, we considered the feedback...against you.  As I mentioned, you start at a disadvantage in the health science space if you don’t have...

  12. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ... cloning procedure adds both V5 and His6 epitope tags to the C-termini of Met proteins, which can be used...relatively confident that the gene from the test species has a similar function to the gene in the reference species...left with Lab Director Doug Warner on the right) has taught courses in biochemistry, molecular cell biology...

  13. Hot Plasmids - October 2020

    Type
    Blog Post
    ...wrmScarlet11 which is less than 60 nt in length to tag the target protein. The team validated this tool ...generated with mRNA display (FingRs), Xue Han’s lab has developed AAV vectors to selectively visualize excitatory...engineered Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-3′ PAM. Get the plasmids at Addgene... human and plant cells. Its small size offers advantages for cellular delivery. Get the plasmids at Addgene...

  14. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ... Choose the AAV capsid or lentiviral prep needed Tags: Identify a specific marker protein Use: Similar...useful in an experiment, and want to see if someone has already created and deposited it with Addgene. Combining...resistance gene(s) used for selection in bacteria. Has Viral Service?: Allows you to filter results to only...

  15. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...et al. "AAV-mediated anterograde transsynaptic tagging: mapping corticocollicular input-defined neural...In order for an AAV particle to infect a cell, it has to come into physical contact with that cell. Since...

  16. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...Addgene is certainly going to have to find ways to tag and curate reagents from these emerging organisms...RNA and can express fluorescent proteins. This lab has created an automated tank for feeding and maintenance...scolopes (Joshua Rosenthal) The Hawaiian bobtail squid has an extreme number of nerve cells and an unusually... injection (hard to do, expensive, not every lab has the capability) with receptor mediated endocytosis...

  17. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...specific edit, such as the insertion of a fluorescent tag or the introduction of a specific mutation, one generally... Broad Institute, John Doench. CRISPR technology has made it easier than ever both to engineer specific...databases annotate the TSS in different ways, and it has been shown that the FANTOM database, which relies...mismatches that can nevertheless still lead to activity has been established, and can be used to identify sites...Perturbation Platform at the Broad Institute and has worked with many Addgenies to help improve the understanding...e141–e141 . https://doi.org/10.1093/nar/gkw583 Rees HA, Liu DR (2018) Base editing: precision chemistry ...

  18. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...studies when used as part of a two part fluorescent tag-then-express system that allows for controlled induction...bloodstream allows for dispersion of the virus but it has to be able to cross the blood-brain barrier to reach...treatments for neurological diseases). The Gradinaru Lab has a lot going on. Here are the key takeaways: Two...

  19. Immunology Research Plasmids and Resources

    Type
    Collection
    ...beta - HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-... MHC-G HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-...MSTP084 HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-... adds a myristoylation sequence and flag-epitope tag to each ORF from the labs of William Hahn and Jean...early stage tumors ARP, MANF, MGC142148, MGC142150 ARMETL1 arginine-rich, mutated in early stage tumors-like...PAFR PTGDR prostaglandin D2 receptor (DP) AS1, ASRT1, DP, DP1, MGC49004 PTGDS prostaglandin D2 synthase..., PGDS2 PTGER1 prostaglandin E receptor 1 (subtype EP1), 42kDa EP1 PTGER2 prostaglandin E receptor 2 (...

  20. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...%}{% end_module_attribute %}{% module_attribute "tag" is_json="true" %}{% raw %}"module"{% endraw %}{%...pAAV-CMV-OCaMP-WPRE pAAV CMV The Lohman lab has a limited quantity of AAVs that can be requested ...end_module_attribute %}{% module_attribute "wrap_field_tag" is_json="true" %}{% raw %}"div"{% endraw %...
Showing: 201 - 220 of 527 results