...discovered that many of my old suitemates stopped talking to each other if I was not there to arbitrate for...lead to hurt feelings, low morale, poor decision making, and stalled projects. You want to identify and...experiments to address a reviewer’s concerns, you are making your hypothesis stronger. When you share data at...miscommunications in the future. Assumptions: Making incorrect assumptions can be a direct cause or ...or consequence of lapsed communication. Making some assumptions about how something should be communicated...know that a person or team should be involved in making a decision and you see no record of their input... an eye out for the phrase “I assumed they were taking care of that”, because that can indicate confusion...

...wise to start working on these skills. Neuroscientists use lots of cool tools While walking around the exhibition...discuss their VR helmets, gait tracking technologies, and brain monitors. Walking around the poster presentation...Neuroscience covers many subfields When I first began looking into the details of SfN17, I expected that almost... mathematical know-how for analysis. If you’re looking to get into neuroscience and you don’t have a background...neuroscience but are afraid you don’t have the right kind of biological expertise to get into the field, there...experiments. This helped reinforce our goals of making more AAVs and educational content to help researchers... suggest a plasmid to us, request that we start making the AAV, or help us produce new educational content...

...collision that’s taking place between CRISPR technology and synthetic biology, the tools he’s making available.... Addgene: Is this what you are working on now? Voigt: We are working on this now, we have ideas, but ...Massachusetts Institute of Technology has been “working on new experimental and theoretical methods to ...large as natural regulatory networks. Addgene: What kinds of things could we do as genetic circuits become... been putting together very large toolboxes and making them available. We’ll also be submitting parts ...objective is to give people the complete toolbox for taking on very large genetic engineering projects. Addgene...you want. The last few years, we’ve really been making tons of parts and characterizing the software to...

...and foreign nationals working in the life sciences community obtain their working visas, permanent resident...national researchers and entrepreneurs who are looking to continue their careers in the United States....we get elected to Congress, we are tasked with working with US immigration in its current state. As of...discussed in this post benefit from the applicant thinking about the process early, keeping good records,...trained scientist. If the candidate is ultimately looking for a role in a different functional area, then...received sponsorship) then he or she can start working in October of the same year. The Industry H-1B ...of US government grants that the researcher is working under in the lab or has won, awards, media reports...

...useful advice about what reviewers in your area are looking for. Remember though, you need to leave them time...The two most important things reviewers will be looking for in your research proposal are originality and...provide different insight into an area - not just working within the established network of your peers. Furthermore...techniques and interact with new people. If you are looking to move to a different university, perhaps in a...re staying in the same lab where you have been working for the last few years, you need to be very clear...Building Your Scientific Network? Check out Our Networking for Scientists Series 4. Become a science communicator...communicator More and more fellowship applications are asking for a 200-300 word explanation of your research...

...expression. Transgenic and knockin mice As the name suggests, transgenic and knockin mice contain an additional...Conditional knockouts or knockins A subset of transgenic mice, conditional knockout/knockin mice, use the Cre-lox...terminology you should be familiar with before working with mouse models, several common techniques used...construct incorporated randomly into the genome, knockin mice have the gene construct inserted at a specific...DNA randomly into the mouse genome. In contrast, knockin strains are generated via homologous recombination...breaks, such as ZFN and TALEN, can used to engineer knockin mice. Co-injection of a targeting vector containing...based systems have been developed for generating knockin mice but because CRISPR/Cas9 engineering favors...

...before you start. Also, avoid unnecessary talking because talking generates microbe-laden aerosols that may...that can be used as your working stock. This way you can go through your working stock vial by vial before.... UV light exposure is also harmful for eyes and skin so keep the light off when the hood is in use. Wipe...wiped with 70% ethanol immediately. Finally, avoid sticking pipettes into solution bottles directly, rather...familiar with the morphology of the cell line you’re working with so that cross-contamination can be identified...indicates that the cells have grown enough, are making their surrounding media acidic through the secretion... their original condition and can replace your working stock if it gets too old or contaminated. This ...

...between scientific achievements. I've found cooking, gardening, hiking, and photography to be very satisfying...and running very early on. Once your system is working, you should maximize data production with the end...strengthen your new model?  PFs need to be regularly thinking about manuscripts. The other items will follow...even a relatively simple experiment. However, by taking this time, you can help ensure that you understand... feel like it's too much work, they don't like speaking, they don't feel like they get useful feedback...you give your talk or poster to enough different kinds of audiences and if you pay attention to how the...more fundamental reason to give presentations - networking! People get to know you through your presentations...

...and unknown. In fact, in less than two years of walking away from the bench, I have worked for seven different...the science policy community is the best part of working in this profession. The folks who work in science... mechanisms in place to ensure your effective networking---and they’re more accessible than ever thanks...trajectory. If there’s one thing everyone loves, it’s talking about themselves--so don’t be shy and reach out... toward solving one problem. It often requires thinking about a problem in a new way through the integration... in a government setting: drafting legislation, making rules, and implementing the policy within the jurisdiction...to start your science policy journey.  In fact, working in science policy doesn’t have to be a complete...

... a diverse range of perspectives and sparking innovative thinking that may benefit your current project...In the pursuit of knowledge and groundbreaking discoveries, the world of academia often emphasizes structured...deeply into your chosen field, don't shy away from looking beyond its confines. The beauty of serendipity ...Practical approaches Engaging in mindful meditation, taking field trips, and spending time outdoors in nature.... These unrelated ideas might hold the key to unlocking new perspectives or even revolutionizing your ...that is adaptable, creative, and poised for groundbreaking discoveries. So, on your academic journey, ... the unexpected — it might just be the key to unlocking the next frontier of knowledge. Matteo Tardelli...

...to a few hundred worms, making the detection of rare events difficult. Dickinson et al. sought to create...markers easy to remove after injection, they added flanking loxP sites and a heat-shock inducible Cre to generate...Figure 1: Self-excising cassette schematic from Dickinson et al. (2015). Used with permission from the Genetics...for the targeted gene. After initial testing, Dickinson et al. tested this system across 7 genes in parallel...modification. Figure 2: Schematic from Dickinson et al. (2015) illustrating the steps to fluorescently...Society of America. Applications of this system Dickinson et al. have developed a modular system that requires...with a Self-Excising Drug Selection Cassette. Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein...

...Regulatory knowledge Networking Blogging Writing Communication Networking Publicity ...space in academic research labs has more students looking for other options. While graduate programs provide...Introduce new perspectives in science and engineering Looking at your research from a broader viewpoint may expose...multiple research studies highlight the importance of taking a break and incorporating EAs into your routine...their required qualifications and skill sets Taking the time to identify and research 2 – 3 potential... a profession sounds interesting, TAKE ACTION! Taking action: Examples of extracurricular activities ...Communication Teamwork Management Leadership Networking University technology transfer office positions...

...Tabor have been working on making E. coli light responsive since 2005, when Tabor was working in Voigt's lab...systems continue to be optimized by Tabor’s lab, making light-controlled gene expression in E. coli easier...two-component systems (TCSs). TCSs consist of a histidine kinase that phosphorylates a response regulator (RR). ...from cyanobacteria to a common E. coli histidine kinase. This hybrid construct senses light, and an obligate... two-component system (TCS). A hybrid histidine kinase/photosensor senses various light inputs, and an...plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter activity persisted...with other pathways. They also reduced system leakiness and increased dynamic range to around 100-fold...

...such as BenchSci and FPbase are working hard to enable researchers by making it easier to find information...manuscript. Luckily, many organizations have been working to provide tools that make it easier to share in-depth...quality controlled plasmids and viral vectors. When working with a plasmid from Addgene, you can be confident... about the unreliability of antibodies. IPI is working to address this issue directly by creating well...Electronic lab notebooks also play a role here by making it easier to retain searchable, thorough notes ...techniques, an important component of reproducibility is making sure that others can understand your analyses and...results that conflict with our original hypotheses. Looking for novelty in our stories, we are additionally...

...are. Here are the questions you should be asking. What kind of control? On, off, tunable, and/or inducible...off protein expression, you are almost certainly working with an endogenous locus. The most straightforward...excision. Essentially, LoxP sites are knocked in flanking a gene of interest (or a single exon) and everything...which is more difficult than a simple knock out. Looking for some permanent knock out plasmids? Check out...suppression of expression is faster and easier than making a knockout and is a great way to temporarily reduce...advantages, such as tissue-specific targeting. Looking to permanently induce expression? Check out our...Destabilizing domains Destabilizing domains (DD) are akin to degron tags, just with a flipped mechanism. When...

... value in depositing and they are increasingly seeking us out on their own to share reagents. I love the...scientists starting their own labs get excited about making their first deposit to Addgene. We are also ...universal resource for information and data. Speaking of the website, we now have a UI/UX team. Last... any more!  Our product and software teams are working in lock step to maximize the services and features... for their dedication, flexibility, innovative thinking, and openness to team work.   Company culture... via connections and even via Twitter. We love working with local organizations like Just-a-Start, the...quality, low meeting, and low email culture. I like working here and I hear from the Addgenies that they do...

... to exactly what you're asking of your audience and when things aren't working out, adjust the ask.  I...: “cognitive load refers to the used amount of working memory resources. There are three types of cognitive... provide detailed evidence of knowledge new to mankind. And you probably understand how much faster it...read this paper than it would be for an undergrad taking their first upper-level class. Even so, it’s quite...more you use, the higher cognitive load you are asking your audience to take on, just to understand you...If there’s a teacher, mentor, or other educator working with your audience, ask! They are experts in what...fly.  Once you can think about everything you’re asking your learners to do, it becomes a lot easier to...

...you a science student or early career researcher looking to break into science communication? Everybody ... festivals and outreach If you are comfortable talking in public, volunteer at science festivals. Or engage...education Do you aim to reach young people? Try networking with your local education community. Get involved...environmental groups, charities and NGOs are often looking for outreach volunteers as well. 4) Blog - Hone...’t you record a podcast or put some effort into making a video? Use your unique talent to communicate ...Association allow you to spend two to six weeks working at media outlets such as the Guardian, BBC Breakfast...education Some people get into science communication by taking a media or journalism course. Courses can help ...

...programs is almost as daunting as the program itself. Picking a mentor comes with many challenges, and the flood...nosy question! The Application Process Broadly speaking, the PhD application process tends to go something... to potential mentors?” “Where do I start with picking universities?” and “How does COVID-19 impact all...program is the best fit for you and your goals. Making a List of Schools Creating a list of schools is...chance to get to know the faculty that you might be working with for the duration of your program and ask any...information about the campus even if you can’t visit. By talking with people at the institution, you can get a better...into what matters to you. It can also be nerve wracking to ask questions in front of other prospective...

... projects and they end up making 30 constructs. They’ve never done any kind of cloning and they don’t ...tools you are working on for plants? Patron: Our lab and others at the Sainsbury are working on a lot of...general. We are funded by a charity and since we are making all the tools for plant research here and validating...problem. MoClo is really a modern toolkit for those working in plants, including many things people already...Addgene: Do you have any particular tips for those looking to get started with MoClo? Patron: The parts will... that you need from the kit and getting started making your own constructs with Golden Gate MoClo. The...network here. She talked about the advantages of talking to people and representing yourself as a real person...