We narrowed to 933 results for: Kin

1. Mouse Modeling, Part 1: Genetically Engineered Mice

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   ...expression. Transgenic and knockin mice As the name suggests, transgenic and knockin mice contain an additional...Conditional knockouts or knockins A subset of transgenic mice, conditional knockout/knockin mice, use the Cre-lox...terminology you should be familiar with before working with mouse models, several common techniques used...construct incorporated randomly into the genome, knockin mice have the gene construct inserted at a specific...DNA randomly into the mouse genome. In contrast, knockin strains are generated via homologous recombination...breaks, such as ZFN and TALEN, can used to engineer knockin mice. Co-injection of a targeting vector containing...based systems have been developed for generating knockin mice but because CRISPR/Cas9 engineering favors...

2. 6 Tips for Grant Writing

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   ...useful advice about what reviewers in your area are looking for. Remember though, you need to leave them time...The two most important things reviewers will be looking for in your research proposal are originality and...provide different insight into an area - not just working within the established network of your peers. Furthermore...techniques and interact with new people. If you are looking to move to a different university, perhaps in a...re staying in the same lab where you have been working for the last few years, you need to be very clear...Building Your Scientific Network? Check out Our Networking for Scientists Series 4. Become a science communicator...communicator More and more fellowship applications are asking for a 200-300 word explanation of your research...

3. 10 Basic tips for mammalian cell culture

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   ...before you start. Also, avoid unnecessary talking because talking generates microbe-laden aerosols that may...that can be used as your working stock. This way you can go through your working stock vial by vial before.... UV light exposure is also harmful for eyes and skin so keep the light off when the hood is in use. Wipe...wiped with 70% ethanol immediately. Finally, avoid sticking pipettes into solution bottles directly, rather...familiar with the morphology of the cell line you’re working with so that cross-contamination can be identified...indicates that the cells have grown enough, are making their surrounding media acidic through the secretion... their original condition and can replace your working stock if it gets too old or contaminated. This ...

4. 9 tips for a successful postdoctoral experience

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   ...between scientific achievements. I've found cooking, gardening, hiking, and photography to be very satisfying...and running very early on. Once your system is working, you should maximize data production with the end...strengthen your new model?  PFs need to be regularly thinking about manuscripts. The other items will follow...even a relatively simple experiment. However, by taking this time, you can help ensure that you understand... feel like it's too much work, they don't like speaking, they don't feel like they get useful feedback...you give your talk or poster to enough different kinds of audiences and if you pay attention to how the...more fundamental reason to give presentations - networking! People get to know you through your presentations...

5. Finding Your Science Policy Path

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   ...and unknown. In fact, in less than two years of walking away from the bench, I have worked for seven different...the science policy community is the best part of working in this profession. The folks who work in science... mechanisms in place to ensure your effective networking---and they’re more accessible than ever thanks...trajectory. If there’s one thing everyone loves, it’s talking about themselves--so don’t be shy and reach out... toward solving one problem. It often requires thinking about a problem in a new way through the integration... in a government setting: drafting legislation, making rules, and implementing the policy within the jurisdiction...to start your science policy journey.  In fact, working in science policy doesn’t have to be a complete...

6. Embracing Serendipity: A Crucial Element in the PhD Journey

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   ... a diverse range of perspectives and sparking innovative thinking that may benefit your current project...In the pursuit of knowledge and groundbreaking discoveries, the world of academia often emphasizes structured...deeply into your chosen field, don't shy away from looking beyond its confines. The beauty of serendipity ...Practical approaches Engaging in mindful meditation, taking field trips, and spending time outdoors in nature.... These unrelated ideas might hold the key to unlocking new perspectives or even revolutionizing your ...that is adaptable, creative, and poised for groundbreaking discoveries. So, on your academic journey, ... the unexpected — it might just be the key to unlocking the next frontier of knowledge. Matteo Tardelli...

7. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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   ...to a few hundred worms, making the detection of rare events difficult. Dickinson et al. sought to create...markers easy to remove after injection, they added flanking loxP sites and a heat-shock inducible Cre to generate...Figure 1: Self-excising cassette schematic from Dickinson et al. (2015). Used with permission from the Genetics...for the targeted gene. After initial testing, Dickinson et al. tested this system across 7 genes in parallel...modification. Figure 2: Schematic from Dickinson et al. (2015) illustrating the steps to fluorescently...Society of America. Applications of this system Dickinson et al. have developed a modular system that requires...with a Self-Excising Drug Selection Cassette. Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein...

8. Extracurricular Activities for a Strong Science Career Path

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   ...Regulatory knowledge Networking Blogging Writing Communication Networking Publicity ...space in academic research labs has more students looking for other options. While graduate programs provide...Introduce new perspectives in science and engineering Looking at your research from a broader viewpoint may expose...multiple research studies highlight the importance of taking a break and incorporating EAs into your routine...their required qualifications and skill sets Taking the time to identify and research 2 – 3 potential... a profession sounds interesting, TAKE ACTION! Taking action: Examples of extracurricular activities ...Communication Teamwork Management Leadership Networking University technology transfer office positions...

9. Synthetic Photobiology: Optogenetics for E. coli

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   ...Tabor have been working on making E. coli light responsive since 2005, when Tabor was working in Voigt's lab...systems continue to be optimized by Tabor’s lab, making light-controlled gene expression in E. coli easier...two-component systems (TCSs). TCSs consist of a histidine kinase that phosphorylates a response regulator (RR). ...from cyanobacteria to a common E. coli histidine kinase. This hybrid construct senses light, and an obligate... two-component system (TCS). A hybrid histidine kinase/photosensor senses various light inputs, and an...plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter activity persisted...with other pathways. They also reduced system leakiness and increased dynamic range to around 100-fold...

10. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    ...such as BenchSci and FPbase are working hard to enable researchers by making it easier to find information...manuscript. Luckily, many organizations have been working to provide tools that make it easier to share in-depth...quality controlled plasmids and viral vectors. When working with a plasmid from Addgene, you can be confident... about the unreliability of antibodies. IPI is working to address this issue directly by creating well...Electronic lab notebooks also play a role here by making it easier to retain searchable, thorough notes ...techniques, an important component of reproducibility is making sure that others can understand your analyses and...results that conflict with our original hypotheses. Looking for novelty in our stories, we are additionally...

11. Plasmids 101: The protein expression toolbox

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    ...are. Here are the questions you should be asking. What kind of control? On, off, tunable, and/or inducible...off protein expression, you are almost certainly working with an endogenous locus. The most straightforward...excision. Essentially, LoxP sites are knocked in flanking a gene of interest (or a single exon) and everything...which is more difficult than a simple knock out. Looking for some permanent knock out plasmids? Check out...suppression of expression is faster and easier than making a knockout and is a great way to temporarily reduce...advantages, such as tissue-specific targeting. Looking to permanently induce expression? Check out our...Destabilizing domains Destabilizing domains (DD) are akin to degron tags, just with a flipped mechanism. When...

12. Summer SciComm Series: Cognitive Load

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    ... to exactly what you're asking of your audience and when things aren't working out, adjust the ask.  I...: “cognitive load refers to the used amount of working memory resources. There are three types of cognitive... provide detailed evidence of knowledge new to mankind. And you probably understand how much faster it...read this paper than it would be for an undergrad taking their first upper-level class. Even so, it’s quite...more you use, the higher cognitive load you are asking your audience to take on, just to understand you...If there’s a teacher, mentor, or other educator working with your audience, ask! They are experts in what...fly.  Once you can think about everything you’re asking your learners to do, it becomes a lot easier to...

13. Celebrating 15 Years of Scientific Sharing

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    ... value in depositing and they are increasingly seeking us out on their own to share reagents. I love the...scientists starting their own labs get excited about making their first deposit to Addgene. We are also ...universal resource for information and data. Speaking of the website, we now have a UI/UX team. Last... any more!  Our product and software teams are working in lock step to maximize the services and features... for their dedication, flexibility, innovative thinking, and openness to team work.   Company culture... via connections and even via Twitter. We love working with local organizations like Just-a-Start, the...quality, low meeting, and low email culture. I like working here and I hear from the Addgenies that they do...

14. Science communication: 9 Strategies to Get Your Foot in the Door

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    ...you a science student or early career researcher looking to break into science communication? Everybody ... festivals and outreach If you are comfortable talking in public, volunteer at science festivals. Or engage...education Do you aim to reach young people? Try networking with your local education community. Get involved...environmental groups, charities and NGOs are often looking for outreach volunteers as well. 4) Blog - Hone...’t you record a podcast or put some effort into making a video? Use your unique talent to communicate ...Association allow you to spend two to six weeks working at media outlets such as the Guardian, BBC Breakfast...education Some people get into science communication by taking a media or journalism course. Courses can help ...

15. PhD Applications After COVID

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    ...programs is almost as daunting as the program itself. Picking a mentor comes with many challenges, and the flood...nosy question! The Application Process Broadly speaking, the PhD application process tends to go something... to potential mentors?” “Where do I start with picking universities?” and “How does COVID-19 impact all...program is the best fit for you and your goals. Making a List of Schools Creating a list of schools is...chance to get to know the faculty that you might be working with for the duration of your program and ask any...information about the campus even if you can’t visit. By talking with people at the institution, you can get a better...into what matters to you. It can also be nerve wracking to ask questions in front of other prospective...

16. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    ... projects and they end up making 30 constructs. They’ve never done any kind of cloning and they don’t ...tools you are working on for plants? Patron: Our lab and others at the Sainsbury are working on a lot of...general. We are funded by a charity and since we are making all the tools for plant research here and validating...problem. MoClo is really a modern toolkit for those working in plants, including many things people already...Addgene: Do you have any particular tips for those looking to get started with MoClo? Patron: The parts will... that you need from the kit and getting started making your own constructs with Golden Gate MoClo. The...network here. She talked about the advantages of talking to people and representing yourself as a real person...

17. Transferable Skills Guide: Identifying Your Transferable Skills

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    ...’ve gained experience working with similar protocols, and look - they’re seeking someone who has, to a... a specific field. While searching for jobs and making connections, avoid falling into an identity trap...retrieved your lucky socks, persevered through a nerve wracking commute complete with a disabled train, and now...this job was made for you. They must have been looking at your resume while they wrote the job description...side of an issue. Maybe you also showed critical thinking skills by dissecting their problem and piecing...organizational skills. If you are responsible for working with lots of students, managing inventories in ...pretty much any other workplace. To get your mind working, take a look at the short list of transferrable...

18. Transferable Skills Guide: External Collaborations

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    ...because you are not (yet) asking for a job or a big favor - you are generally asking for some advice and guidance...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...many methods and machines one team can learn so linking together expert teams can push a scientific project...graduate studies at Karolinska Institutet I started working with other groups. First within my department where...meet collaborators  for lunch, make a point of talking to them at a seminars, or even set up regular meetings...much more effective than simply sending a message asking how things are going. Science careers where the...studies in regard to reaching out to new people, checking in regularly, and maintaining useful notes that...

19. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    ...authors’ compared nicking the top strand first followed by the bottom strand versus nicking the bottom strand...position. The “sloppy” PCR reaction is created by making some or all of the following modifications: 1) ...packaged into phage and then extracted. Besides taking a couple days to generate the phage-derived uracil...ready to be tested in a functional screen of your liking. This method is similar to site-directed mutagenesis...with a twist since it relies on the sequential nicking, degradation, and PCR-generation of a mutant version...Supplementary Note 1 in Wrenbeck et al for details on how nicking was optimized. How should I design my mutagenic...gene of interest has a BbvCI site(s). If so, the nicking mutagenesis protocol will still work if the orientation...

20. How to Negotiate a Successful Lab Start Up Budget

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    ...step by step instructions on creating a budget, making decisions based on that budget, and negotiating...timeline Start with your thesis in mind. Imagine the kind of data your dream discovery will need, and how ...should reflect salaries in the region you’ll be working. At this point, your budget bottom line costs may...position. This is important to ensure that you’re looking at institutes that have the means to support your...values. All too often, we see early investigators making unnecessary short term sacrifices that hurt their...dependent on a pathology microtome/cryostat, then making a careful decision on the condition and availability... and limitations. Everything is negotiable. Try asking for discounts, collaborations, access to internal...