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... Map & Network (CeNGEN) The CeNGEN goal is to establish a comprehensive gene expression atlas of every...publication for the worm community where one can publish brief novel findings. These results are normally...results. This a great resource for scientists to publish findings that don't fit into larger publications... that can be referenced. In addition the data published in microPublications is curated and added to other...but there are Drosophila and zebrafish articles published there as well.   Worm Development Dynamics Database...used for introducing point mutations that was published in a microPublication (Schwartz and Jorgensen,...

... impossible to interpret. Polishing the fiber optics with diamond polishing paper of sequentially increasing... second key point; the fiber optics need to be polished to significant smoothness to prevent drastic loss...its output with a light meter before and after polishing. Efficiency of the implant should be at least ... output. The same considerations apply to the polishing and preparation of the patch cable (although you...it’s best to try those that have already been published first. Because behavioral optogenetics is performed...

...intellectual property, it worked away in isolation. It published a paper a year (or less) and limited the exchange...public and to other academic labs. Being unable to publish or attend conferences makes it very challenging...As a Master’s student, she had less pressure to publish and more freedom to attend conferences, but after...occasionally thinking about how we could bypass the establishment hiring system altogether. We had a lot of ideas...restricted to science jobs. Keira’s an optimist, I’m a realist, and together we make a pretty good team. We have... – although we didn’t figure out how we would accomplish the color change until a few months later. RevBio...

... thanks to our guest bloggers Alissa Lance-Byrne and Alex Chavez! Alissa Lance-Byrne is a graduate student...This post was contributed by guest bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss...gene or introduce a desired genetic alteration. Listen to Our Podcast Interview with Alex Chavez ... induce Cas9 to make edits (AC, WLC, and JQ, unpublished results). An additional benefit of the use of...PMC4265475. Additional Resources on the Addgene Blog Listen to Our Interview with Alex Chavez Learn about Sequencing...

...access to a list of posters until the day-of, take a moment to step aside to read through the list at the ... a day in advance. Dress for the venue and professionalism of the conference, and remember to wear comfortable...about the relationship of your work to recently published papers or “hot topics,” so be sure to be well-...strategically use your time. Don’t have access to a list of posters at all? No problem! At the beginning ...traverse the sea of posters If you come prepared with a list of posters of interest, then you’ve already set ...

... can be helpful. Publishing: As a postdoc at AstraZeneca, you are expected to publish, but this can vary...prerequisite “industry experience” that I’ve seen listed as a requirement on some industry job postings....vary depending on which company you choose. If publishing is important to you, make sure that your project... Regeneron, and Genentech, among others, and the list is growing. Some post positions as they come up,... Novartis, have specific web sites for postdoc listings, so the method of posting varies between companies...

... new accomplishments to add. Obtain copies and translations of documents that are not in English. Create...development, consulting, policy, big data and the list goes on and on. As you think about your career (... to obtain permanent residence. The recently established case Matter of Dhanasar provides the three-prong...qualify, you need to satisfy 3 out of the 10 criteria listed below. An international or national award for ...therapeutics to cure the cancer. Authorship:  Publishing books, journals online and/or in print. Having...demonstrate outstanding achievement Evidence of published material in professional publications written ...

... already answered this question and used it to publish!  Working with AAV vectors in neuroscience Let...performance in our system and yet none of that data was published.    Fig. 1: Testing of three different ... for your system or to successfully replicate published works. Of particular importance to these efforts...build on others’ results is available not through published manuscripts, but through word of mouth or old ...asked your team members or colleagues: How much unpublished AAV data do you have sitting in computers and... O’Connor, D. M., Lutomski, C., Jarrold, M. F., Boulis, N. M., & Donsante, A. (2019). Lot-to-Lot Variation...

...made a table that listed each protein in the class, then for each protein we listed all the studies that...include every piece of information that was ever published. Obviously this isn’t possible. So, define your...people cared about. I also used reviews to get a list of research papers that I needed to read. Once I... page as possible. This could be in the form of lists, streams of consciousness, or anything else. Like.... Curate and present some useful data Typically lists and pictures are the most useful parts of reviews...

...specific subjects in the interview. Happy Listening! Click here to listen! Podcast breakdown: (Time : Topic) ...Harvard Medical School researcher Connie Cepko. Listen to the podcast below to hear all about the research... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer... Plasmid Tools Being Developed in the Cepko Lab Listen to our Interview with Michael Koeris Get Cepko ...

...endogenous homologous recombination enzymes can also accomplish the desired genomic modification. This technique... which results in a merodiploid. This can be accomplished by selecting for an antibiotic resistance marker...counter-selection system relies on the sacB gene product of B. subtilis (Hmelo et al., 2015). The sacB gene product is...binding site (using the online calculator from the Salis group) (Espah Borujeni et al., 2014). Replaced the...scarless modification of bacterial genomes. It can accomplish large deletions, insertions, and even precise..., Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation rate is controlled by coupled trade-offs...

...get people to remember you). What better way to accomplish all of these things naturally than to give a ... talk without looking at their slides or notes. Listen to their stories and narratives (see below “Tell...presentation appropriately.  Find out who will be listening.  Are they early, mid, or late career?  Will many... Tell a story We are biologically programmed to listen and remember better when we hear material delivered...should be very simple. The goal is to get the listeners to be thinking “What happens next?”  Describe .... During your practice rounds, encourage your listeners to ask questions and record those that were asked...

... Video Production Specialist and Arthur Guerra who is a Customer Support Specialist. Both of these Addgenies...Hola! Que tal? My name is Brandon Solis, and I am a rising senior studying Sociology and Dance at Skidmore...of challenging what I am used to. Brandon Solis, marketing intern. Something that I struggle ... Brandon Solis...

...which he submitted a phony paper to a list of 304 open access publishers. By the time the magazine went to...world. 4)      The Annals of Internal Medicine published systematic reviews on an agent used to promote... into account. 7)     Science’s John Bohannon published an article describing a sting operation in which... publications, although many don’t necessarily publish in OA journals themselves. A good number of those...

...is busy and has a To-Do list long enough that there is no hope of accomplishing it all. How do we encourage...teams at Addgene will forgo their regular To-Do lists (other than emergency items) and focus on a single... Hack Days, and we’ve seen great success at accomplishing “we-should-really-make-time-for-that” type projects...wonderful tradition for university departments to establish, with the goal of fostering better communication...

...along the way in this ongoing series of blog posts. Listen to our podcast interview with Harvard Medical School...’ – inspire you.” “Also a mentor can give you realistic expectations. (Don't think you can finish that...is instructive (and entertaining) to review this list of bad mentor behaviors. Sadly, it is Yoda who is... concrete goals and helps you reach your goals Listens well and communicates clearly and openly Gives ...like to be validating without being coddling, to listen without being taken advantage of, and to respect...

...CRISPR-based phenotypic screen datasets. Original published CRISPR screen data is curated and deposited in...in BioGRID ORCS to allow facile queries of published data by biomedical researchers and ready access to...identifiers and links provided, in addition to a list of individual screens curated for the publication... approach serves to maintain consistency with published screen interpretations and allows users to query...curators at [email protected] to submit their published data for rapid curation review and upload. In ...

... full picture and listen for hints at the same concerns from multiple people. Listen to what they say,...path and help you pursue any career alternative? Listen to our podcast interview with Harvard Medical School...Ask alumni of the lab. Most labs have websites listing alumi and it is easy to use publication records... end of this blog post. This document includes a list of questions to ask your potential advisors and ...

... a recent plasmid deposit please sign up here. Listen to the Hot Plasmids Podcast!  MarathonRT: A highly...transcriptase Article contributed by Will Arnold  Listen to the MarathonRT podcast segment Researchers from... surface Article contributed by Marcy Patrick  Listen to the nanobody toolkit podcast segment Nanobodies...Caspex  Article contributed by Susanna Bachle  Listen to the Caspex podcast segment Steven Carr’s lab... C-BERST Article contributed by Klaus Wanisch  Listen to the C-BERST podcast segment Similarly to the...

...seems like another dragon to slay on the way to publish your work in a scientific journal. The peer-review...touchstone of the scientific method, ensuring that published work meets the accepted standards of reporting...read, I pay more attention to details and use a checklist for individual sections of the paper (see figures...authors put in the manuscript. As a non-native English speaker, I sometimes miss the sensitivity to judge...sharing whatever she knows with whoever cares to listen. In March 2020 she will start her own research ...