We narrowed to 15 results for: LIS;

...pnas.0700293104 PMID: 17360345 Berglund, K., Clissold, K., Li, H. E., Wen, L., Park, S. Y., Gleixner, J....physrev.00009.2017 PMID: 29351511 Armbruster, B.N., Li, X., Pausch, M.H., Herlitze, S., Roth, B.L. (2007... M., Gautam, D., Scarselli, M., Ruiz de Azua, I., Li, J. H., Rosemond, E., Ma, X., Gonzalez, F. J., Armbruster...srep14366 PMID: 26399324 Vardy, E., Robinson, J. E., Li, C., Olsen, R. H. J., DiBerto, J. F., Giguere, P....experiments in this species may be entirely due to back-metabolism of CNO to clozapine, demonstrating the need for...activity, and do not seem to have the same back-metabolism issues as CNO and are attractive alternatives...controlled with cell-type specific promoters. Table 4 lists some common promoters found in chemogenetic receptor...

...Bartolomei, M. S., Ferguson-Smith, A., Li, D., Yang, L., Wu, Y., & Li, J. (2015). CRISPR-Cas9-Mediated Genetic...: 25494202 Liu, X., Zhang, Y., Chen, Y., Li, M., Zhou, F., Li, K., Cao, H., Ni, M., Liu, Y., Gu, Z., Dickerson...: 23287718 Cui, Z., Tian, R., Huang, Z., Jin, Z., Li, L., Liu, J., Huang, Z., Xie, H., Liu, D., Mo, H...., J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., Cradick, T....V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee, M. J., &...29160308 Hess, G. T., Frésard, L., Han, K., Lee, C. H., Li, A., Cimprich, K. A., Montgomery, S. B., & Bassik...., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. ...

...4939-1862-1_10 PMID: 25408407 Dixit, A., Parnas, O., Li, B., Chen, J., Fulco, C. P., Jerby-Arnon, L., Marjanovic...j.cell.2014.09.029 PMID: 25307932 Glass, Z., Lee, M., Li, Y., & Xu, Q. (2018). Engineering the delivery system...exon coding for an essential protein domain could abolish protein activity and essentially function as a ...with identical genomes. If your end goal is to establish an edited cell line, for example, you will likely...

...Link opens in a new window) Liu, D., Li, T., Liu, L., Che, X., Li, X., Liu, C., & Wu, G. (2024). Adeno-associated...AAVs cannot enter the lytic cycle, and instead establish latent infections by integrating into the host...

...doi.org/10.1073/pnas.94.16.8640 PMID: 9238030 Wu, X., Li, Y., Crise, B., & Burgess, S. M. (2003). Transcription...vectors, they offer the benefit of having well-established protocols for fast and efficient generation of...large-scale, high-titer preps. This is thanks to established retroviral production cell lines that are able...

...., Zhao, C., Zheng, Z., Shu, Y., Wu, X., Lei, J., Li, Y., Zhang, W., Yang, C., Wu, K., Wu, Y., Ho, S.,...remain the most popular choice due to their well-established methods, relative simplicity of production, and...

...two most common types of ELISAs are direct or sandwich ELISAs. For direct ELISAs, an antigen or protein ...expression and assay efficacy. ELISA Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay that...differentiate between proteins. HRP is also used in ELISAs, which only measure a single antibody signal. Here...relative amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein...Quantification methods, such as western blots or ELISAs, are used to quantify relative amounts of protein...standard curves to quantify exact amounts of proteins. ELISAs quantify a single protein per assay and typically...signaling molecule. Figure 4: A direct sandwich ELISA, which uses a capture antibody and a primary conjugated...

... along with a more complete list of common sequencing primers. These lists are provided for your reference...Find a list of universal sequencing primers and other common sequencing primers. Educational...sequence verification over the years. Below is a list of popular universal primers available from most...find your plasmid’s page and see what primers are listed under "5' sequencing primer" and "3' sequencing...

...the insert or other regions of the plasmid. For a list of commonly used primers, check out our sequencing...expression of an endogenous gene. This is frequently accomplished through expression of an shRNA targeting the...edit genomes. Genome editing is most commonly accomplished using CRISPR technology. CRISPR is composed ...-related information and a more extensive strain list. Strain Vendor(s) Genotype BL21 Invitrogen; New ...Sequence FLAG DYKDDDDK HA YPYDVPDYA His HHHHHH Myc EQKLISEEDL V5 GKPIPNPLLGLDST Xpress DLDDDDK or DLYDDDDK ...

...Yeast Toolkit (MYT) Yeast Expression, CRISPR Tom Ellis 96 plasmids to extend the MoClo-YTK (Dueber) for... Yeast GPCR-sensor Toolkit Yeast Expression Tom Ellis Plasmids for constructing highly-tunable GPCR-based... standards. CyanoGate Kit Bacterial Expression Alistair McCormick Kit containing 96 parts and acceptor...Komagataeibacter Tool Kit (KTK) Bacterial Expression Tom Ellis DNA parts to create multigene constructs expressed...

...relevant to the phenotype being studied. Examples of published selection mechanisms for lentiviral CRISPR libraries...cells Sequence those cells that pass (“win”) Get a list of genes involved in the selection NEGATIVE screen...between experimental and control cells Generate a list of gRNAs that disappear with addition of the selection...

...depression, drug addiction, and more. Below is a list of commonly used microbial opsins, including a brief...each and some examples of relevant variants. This list is not exhaustive — please browse our Optogenetics...Bamberg, E., Nagel, G., & Deisseroth, K. (2005). Millisecond-timescale, genetically targeted optical control...

...using Addgene’s materials, and career advice. Playlists include: Protocols Careers How-to Blog Read about...

...ends can still be fused in a seamless manner. To accomplish this, you can transform into yeast the fragments...

...PMID: 36348415 Stewart, S. A., Dykxhoorn, D. M., Palliser, D., Mizuno, H., Yu, E. Y., An, D. S., Sabatini...