We narrowed to 220 results for: LRE

1. A Needle in a Base-Stack: Cas9 Structural Biology

   Type

   Blog Post

   ... guide RNA. But how to test DNA bases that are already snugly paired to each other? At this point, the...

2. How to Deposit Your Plasmids with Addgene

   Type

   Blog Post

   ...and Add Another Gene/Insert". The data you have already entered will be saved and the page will open a ...

3. Antibody Validation for Flow Cytometry

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   Blog Post

   ...it may be helpful to find an antibody that has already been validated for flow cytometry through this ...

4. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

   Type

   Blog Post

   ...enough. Casseus Law suggests that if you are not already reviewing papers that you should start contacting...

5. Troubleshooting and Optimizing a Western Blot

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   Blog Post

   ...lower lot-to-lot variability. If you’re running an already-developed assay, and you have faint or no bands...

6. 22 Hot Plasmid Technologies from 2014

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   Blog Post

   ...to red when it is oxidized. The Timer tool has already been used by several groups to monitor protein ...

7. Protocol - How to Create a Bacterial Glycerol Stock

   Type

   Protocol

   ...want to make more plasmid DNA, the plasmid will already be in your desired bacterial strain and you will...

8. Protocol - Over-Agar Antibiotic Plating

   Type

   Protocol

   ...Learn how to add antibiotic to a plate after it's already been poured and solidified....

9. Fluorescence Titering Assay

   Type

   Protocol

   ...suitable with your protocols. FBS can be purchased already heat-inactivated, or it can be inactivated in the...

10. General Transfection

    Type

    Protocol

    ...suitable with your protocols. FBS can be purchased already head inactivated or it can be inactivated in the...

11. Viral Vectors 101: Systemic Capsids

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    Blog Post

    ... Cell Reports, 34(13). https://doi.org/10.1016/j.celrep.2021.108754 Ravindra Kumar, S., Miles, T. F., ...

12. Lentivirus Production

    Type

    Protocol

    ...suitable with your protocols. FBS can be purchased already heat inactivated or it can be inactivated in the...

13. Colony Formation Titering Assay

    Type

    Protocol

    ...suitable with your protocols. FBS can be purchased already heat-inactivated, or it can be inactivated in the...

14. Pipetting Protocol

    Type

    Protocol

    ...microcentrifuge tube, a bottle etc.). If there is already other liquid in the receiving tube, place the pipette...

15. Protocol - How to Run an Agarose Gel

    Type

    Protocol

    ...place the gel tray at 4 °C earlier so that it is already cold when the gel is poured into it. Loading Samples...

16. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type

    Protocol

    ...DNA from one vector to another as long as it is already bounded by restriction sites that are also present...

17. 27 Hot Plasmids from 2016

    Type

    Blog Post

    ...growing collection of dCas9-VPR plasmids, which already includes dSaCas9-VPR in an AAV transfer vector ...

18. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type

    Protocol

    ... Add protease inhibitors to 1X if this has not already been done. We typically start with about 250 mL...

19. AAV Production in HEK293 Cells

    Type

    Protocol

    ...suitable for your protocols. FBS can be purchased already heat-inactivated, or it can be inactivated in the...

20. CRISPR Library Amplification

    Type

    Protocol

    ... to weigh the empty tube beforehand! If you’ve already gone too far, weigh an identical empty tube. It...