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  1. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...viruses rely on the help of human proteins in their lifecycle, identifying these potential human helpers of ...Melanie Brewer (right) from the University of California, San Francisco. Manon Eckhardt and Melanie Brewer...

  2. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...at The Scripps Research Institute in La Jolla, California. She joined Elsevier after her postdoctoral studies...range of primary research and review journals across life and physical science, and editorial contribution...

  3. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...peroxisome studies since their peroxisomes will proliferate upon a media change into methanol and peroxisomal...peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation K. phaffii’s genome has...

  4. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...saw these two papers,” said the University of California Berkeley’s Jennifer Doudna. “They are the first...continue to be a primary preoccupation of her California lab and many others. “Those off-target effects...

  5. Science Career Options

    Type
    Blog Post
    ...overtime and weekends, part time or full time) and what life is like for someone who currently in the position...online job websites to see what requirements or qualifications are needed for the role, as this will give ...

  6. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...SLIC cloning process, a gene of interest is PCR amplified to add 5′ and 3′ homology regions from the target...assembly with SLIC. Multiple fragments are PCR amplified to add 5′ and 3′ homology regions to the adjacent...

  7. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...students focus on the application process, and simplify access to academic resources, we created STEAMid...Chemical Biology Program at Harvard. Roo wants to simplify access to academic resources among economically...

  11. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ... by using primers that are vector specific but amplify a potential insert. If you’re using insert-specific...no insert in the plasmid, you won’t be able to amplify anything. Like any experiment, you’ll want the ...

  12. Hot Plasmids - May 2022

    Type
    Blog Post
    ... Plasmids here! Xiong, et al. eLife. 2021. https://doi.org/10.7554/eLife.64631  Goertsen D, et al. Nature...

  13. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...genome editing.  For a few reasons, Cpf1 is a simplified system for editing multiple targets compared ...s ability to process its own pre-crRNA arrays simplifies the crRNA cloning process. For cloning, Zetsche...

  14. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...Conventional thermal cycling is then performed to amplify the target using PCR. Instead of tracking the reaction...assays include a forward and reverse primer to amplify the target locus, as well as a reference probe ...

  15. dTAG - You're it!

    Type
    Blog Post
    ...is not necessary for the proliferation of basal-like breast cancer cells. eLife. September 2017. 6: e26693...

  16. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...the scientific method to everyday problems in your life. This tactic is a good way to see how transferable...resolve issues effectively does not mean I am qualified to be an astronaut. But, knowing that this skillset...

  17. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...antibodies are polyclonal, as their function is to amplify the signal from a primary antibody; the ability...target protein thereby greatly increasing signal amplification.  When to stay away from polyclonal antibodies...

  19. Summer SciComm: Preprints

    Type
    Blog Post
    ... Biology in a position working with preprints in life sciences. He also posted two preprints of his own...proposal for the future of scientific publishing in the life sciences. PLoS Biol. https://doi.org/10.1371/journal.pbio...

  20. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...secondary antibody is ideal as it will help to amplify the signal. Some applications use either direct...that a secondary should be used when signal amplification is needed.  Species  Next, check the species...
Showing: 201 - 220 of 471 results