Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 44 results for: Lif;

Clear keywords
Showing: 1 - 20 of 44 results

  1. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...85054 pLifeAct_mScarlet_N1 Actin filaments LifeAct mScarlet Dorus Gadella 85056 pLifeAct_mScarlet-i_N1 ...54491 mCherry-Lifeact-7 Actin filaments LifeAct mCherry* Michael Davidson 54610 mEGFP-Lifeact-7 Actin filaments... 189767 pLifeAct-mScarlet3_N1 Actin filaments LifeAct mScarlet3 Dorus Gadella 127434 pLifeAct-miRFP670nano...mCherry Christien Merrifield 36201 pLifeAct-mTurquoise2 Actin filaments LifeAct mTurquoise2 Dorus Gadella 36202...EGFP Robert Singer 40908 pDEST/LIfeAct-mCherry-N1 Actin filaments LifeAct mCherry Robin Shaw 21948 pCAG-mGFP-Actin...miRFP703 Vladislav Verkhusha 79993 pLifeAct-miRFP703 Actin filaments LifeAct miRFP703 Vladislav Verkhusha 79994...mCherry William Bement 164459 LifeAct-mGreenLantern Actin filaments LifeAct mGreenLantern Gregory Petsko...

  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... that reports with lifetime and intensity changes A turquoise fluorescence lifetime-based biosensor for...for detecting signaling activities in live cells. eLife. 2018 Jul 3;7. Jin Zhang Calcium Yellow protein ...calcium indicators for imaging neural activity. eLife. 2016 Mar 24;5. pii: e12727. Douglas Kim , GENIE...biosensor for in vivo applications across the tree of life. PLoS Biol. 2022 Sep 6;20(9):e3001772. Robert Campbell...for detecting signaling activities in live cells. eLife. 2018 Jul 3;7. pii: 35458 Jin Zhang cAMP (cyclic...fluorescent glucose indicators for living cells (Glifons) Green Fluorescent Protein-Based Glucose Indicators...fluorescent glucose indicators for living cells (Glifons) Development of red genetically encoded biosensor...

  3. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...expressing Rep2 and PHP.eB Cap controlled by tTA-TRE amplification system Viviana Gradinaru 103006 pUCmini-iCAP-PHP.S...expressing Rep2 and PHP.S Cap controlled by tTA-TRE amplification system Viviana Gradinaru 127847 pUCmini-iCAP-PHP.V1...expressing Rep2 and PHP.V1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175004 pUCmini-iCAP-AAV.CAP-B10...expressing Rep2 and B10 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175005 pUCmini-iCAP-AAV.CAP-B22...expressing Rep2 and B22 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 185136 pUCmini-iCAP-AAV.MaCPNS1... Rep2 and MaCPNS1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 185137 pUCmini-iCAP-AAV.MaCPNS2... Rep2 and MaCPNS2 Cap controlled by tTA-TRE amplification system Viviana Gradinaru All Available AAV Packaging...

  4. CRISPR Guide

    Type
    Collection
    ...experiments and must be amplified before use (Figure 8C), following the amplification protocol specified by... by the depositing lab. After amplification, you’ll need to use next-generation sequencing (NGS) to verify...verify that the composition of your amplified library matches that of the original library. Next, you’... copies of the aptamer improves stability and amplifies the signal for better imaging of genomic loci,...SHERLOCK (and SHERLOCKv2) allows for greater amplification and quantification and can be used on lateral... Innovative Genomics Institute, University of California, Berkeley. Plan Your CRISPR Experiment Resources... DNA PCR P olymerase C hain R eaction; Used to amplify a specific sequence of DNA pegRNA P rime E dit ...

  5. mTOR Pathway

    Type
    Collection
    ... regulator controlling cell growth and proliferation. mTOR, a serine/threonine kinase, is found ...conditions needed for uncontrolled cell growth and proliferation, including an abundant supply of macromolecules...energy, as well as the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears...upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids Click on a name to find...

  6. Fluorescent Proteins: FRET

    Type
    Collection
    ... the acceptor. Alternatively, the fluorescence lifetime (the time between excitation of the fluorophore...of the donor can be measured using fluorescence lifetime imaging microscopy (FLIM) or FLIM-FRET, where ...energy transfer through FRET reduces the donor lifetime. FRET is often used to study (1) protein-protein.... https://www.leica-microsystems.com/science-lab/life-science/what-is-fret-with-flim/ (Link opens in a...

  7. Antibody Guide

    Type
    Collection
    ...Affinity Reagents Handling Visualization Signal Amplification Application Overview Quantification Methods ...present on the Fc region. This allows for the amplification of signals by having multiple secondary antibodies...on the antibody and conjugate chosen. Signal Amplification The amount of signal needed from a specific ...region of primary antibodies allows for signal amplification through the indirect detection method. In the...released from the complex and can be sequenced and amplified for analysis. Overview of application Process ...

  8. AAV Packaged on Request

    Type
    Collection
    ...preps, which includes MTA facilitation, DNA amplification, and high-quality quality control measures such...legal approval and an implementation letter DNA amplification Viral vector production Density centrifugation... Quality is our priority. During this time, we amplify DNA, produce the prep, and perform comprehensive...

  9. Immunology Research Plasmids and Resources

    Type
    Collection
    ... LSH-R LIF leukemia inhibitory factor (cholinergic differentiation factor) CDF, DIA, HILDA LIFR leukemia...factor receptor alpha CD118, FLJ98106, FLJ99923, LIF-R, SJS2, STWS, SWS LRSAM1 leucine rich repeat and... GIF, GLIF, MMIF MLN motilin MGC138519 MLNR motilin receptor GPR38, MTLR1 MPL myeloproliferative leukemia... function as signaling proteins involved in proliferation, cell differentiation, and apoptosis. Plasmid...ACTH, CLIP, LPH, MSH, NPP, POC PPARA peroxisome proliferator-activated receptor alpha MGC2237, MGC2452, NR1C1... NR1C1, PPAR, hPPAR PPARD peroxisome proliferator-activated receptor delta FAAR, MGC3931, NR1C2, NUC1,...NUCI, NUCII, PPAR-beta, PPARB PPARG peroxisome proliferator-activated receptor gamma CIMT1, NR1C3, PPARG1...

  10. CRISPR References and Information

    Type
    Collection
    ...selection of organisms. From the Database Center for Life Science (DBCLS) in Japan. Developed by the Kumiko...Download protocol Addgene CRISPR pooled library amplification CRISPR pooled libraries Protocol at Addgene ...CMV-EGFP PDF, 2.4 MB Zhang GeCKO pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system...

  11. Tetracycline Inducible Expression

    Type
    Collection
    ...transcriptional and proliferative state of each cell in a population. Proliferation history is monitored... tissue distribution, low toxicity, a known half-life (24 hours), and is relatively inexpensive. The induction...

  12. Cell Migration Consortium Plasmids

    Type
    Collection
    ...University of Virginia Denise Montell , University of California, Santa Barbara Martin Schwartz , Yale University...University of Illinois Ron Vale , University of California, San Francisco Browse Plasmids ID Plasmid Gene...

  14. Distribution to Industry

    Type
    Collection
    ...portion of Addgene's collection is available to qualified for-profit entities. Addgene reserves the right...right to determine if an entity is qualified to receive materials from our repository. All purchases are...

  15. AAV Molecular Tools

    Type
    Collection
    ...transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Viviana Gradinaru 99121 pAAV-ihSyn1...transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Viviana Gradinaru 117383 TRE-DIO-eYFP...

  16. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pHR-FUSN-mCh-Cry2WT FUS SFFV ALS Clifford Brangwynne 101224 pHR-FUSN-mCh-Cry2olig FUS SFFV ALS Clifford Brangwynne 101226...SFFV ALS Clifford Brangwynne 122441 pHR-FUSN-miRFP670-Cry2WT FUS Cry2WT, miRFP670 SFFV ALS Clifford Brangwynne...ALS2 ALS2 T7 Primary lateral sclerosis juvenile Cliff Tabin 19457 pcDNA5/FRT/TO HSPA1L HSPA1L CMV Parkinson's...101226 pHR-HNRNPA1C-mCh-Cry2WT HNRNPA1 SFFV ALS Clifford Brangwynne 101792 pMXs-mPKR-FLAG-IG EIF2AK2 Flag...HNRNPA1C::mCherry::SspB HNRNPA1 mCherry SFFV ALS Clifford Brangwynne 122669 M31: pHR-SFFVp- TDP-43C::mCherry...mCherry::SspB TARDBP mCherry SFFV ALS Clifford Brangwynne 123600 pcDNA6-N-3XFLAG-Vcp VCP Flag ALS Bruce...HNRNPA1-miRFP670-TRF1 HNRNPA1 miRFP670 SFFV ALS Clifford Brangwynne 228034 pDONR223 Rab25-STOP RAB25 T7...

  17. Open Enzyme Collection

    Type
    Collection
    ...polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA, and ...for fixing damaged and degraded DNA prior to amplification, enzymes for cutting protein sequences to remove...

  18. Neurodegeneration Research Collection

    Type
    Collection
    ...more likely a combination of gene variation and lifestyle or environmental factors. For example, variations...2019) pLVX-UbC-rtTA-Ngn2:2A:Ascl1 (Herdy et al. Elife. 2019) Use plasmids expressing SOX10, OLIG2, and...

  19. Plasmids for Stem Cell Research

    Type
    Collection
    ... Stem Cell Research Plasmids Stem cells are proliferative, unspecialized cells with the capacity to differentiate...optimize the conversion of fibroblasts into neurons. Elife. 2019 May 17;8. pii: 41356. Gage Fibroblasts iPSCs...

  20. Brain Initiative Collection

    Type
    Collection
    ...promoter that contains a positive feedback loop for amplifed expression of the tTA Viviana Gradinaru 99121-...promoter. Contains a positive feedback loop for amplifed expression of the tTA Viviana Gradinaru 99130-...
Showing: 1 - 20 of 44 results