We narrowed to 564 results for: PAC;

... and impact the field - its encouraged to dream big! It is crucial that the goal and the impact of the...research plan Most grant applications restrict the space available to describe the proposed work. Thus, every...

...weight (53 kDa) when run on an SDS-PAGE gel. Cancer Impact p53 is the most frequently mutated gene (>50%) ...disorder known as Li-Fraumeni syndrome. Recently some impacts of p53 mutations have been characterized as gain-of-function...

.... https://pubmed.ncbi.nlm.nih.gov/24390935/ Hypercompact genome editor CRISPR-Casɸ By Melina Fan The ...toolbox with their discovery of CRISPR-Casɸ, a hypercompact genome editor from the Biggiephage clade of ...the Doudna Lab. Scientists looking for more compact genome editors due to delivery constraints and ...

... arrived; you’ve painstakingly cared for your packaging cell line, prepped your DNA, transfected and harvested...original plasmid which can over-inflate results. A packing vector deficient in reverse transcriptase will ...integration events. In addition, promoter choice can also impact transgene expression. The most accurate method ...

...will likely be spending a few hours on your feet. Pack water and snacks to keep you energized throughout...session, bring extra clips to hold up your poster, and pack a few business cards to tack up next to your poster...hearing the highlights so that you can appreciate the impact of the project as a whole. In addition to time ...

...are sharing stories of our repository’s positive impact on science and scientists around the globe. Our...CRISPR plasmids to labs in ~100 countries.  The impact Addgene’s CRISPR collection contained 197 plasmids...citations.  CRISPR is a story that centers around the impact of science, but our data makes it clear that it...

...Clutter not only makes it difficult to clean your workspace properly, but can also disrupt the laminar air...materials into the hood. This will give you enough space to move around freely without causing spills. Also... than 80-85% confluent because cells like their space. If they reach confluence, they stop growing (contact...

...a 10 cm dish in 15- 20 minutes- a far more rapid pace than using cloning rings. A picture of a 60 mm dish...the plates that do not appear to be yielding well-spaced clones. For rapidly growing cells we sometimes ...the use of an electronic pipettor with variable spacing (such as the Viaflo from Integra Biosciences) to...

...other people to do that too. We don’t have the capacity to send tools out to people ourselves, but we’...committees, and how the culture of the university impacts the number of women reaching senior positions. ... those amazing students that want to have a big impact on the world and tell them to work in plant science...

...top):  A CRISPR RNA (crRNA) comprising a 32 nt spacer sequence specifying the target site. Four proteins...INTEGRATE using a single plasmid containing a multi-spacer CRISPR array. Workflow and technical considerations...INTEGRATE system follows a simple workflow: Clone spacer(s) into the CRISPR array Clone desired DNA cargo...

...sequences, the amino acid sequences RSIAT and RPACKIPNDLKQKVMNH have been used successfully (Kerppola, 2006...expressed transiently by transfection or stably impacts the success of BiFC experiments. In either case...//doi.org/10.1101/pdb.prot076497 Kim Y, Jung JP, Pack C-G, Huh W-K (2018) Global analysis of protein homomerization...

...interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com Science communication... lectures, presentations, etc… Low (except for space) Real-time audience engagement and response. High...fear. Can be discouraging to build audience. Need space & higher entry barrier for audience versus passive...

..., including the E1 region). The space in the viral particle’s capacity that is made available by this ...genome, and adenoviral vectors all have a genomic capacity that is much higher than other viral vectors (...

...antibody (HCab), which are unique to camels, llamas, alpacas and other camelids. In terms of structure, HCabs...have done the hard work for you by immunizing an alpaca, isolating HCab antibodies from its serum, and ...for generating nanobodies are outlined below; no alpaca required. Decide Which Nanobody To Use: The table...

... Viral Vector Packaging Service Viral Production Viral Production Jump...cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab . Cell culture...

...) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM sequence). The 3-5 nucleotide...about 1 kilobase smaller than SpCas9, so it can be packaged into adeno-associated virus (AAV).  At 984 amino...edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target...

...discovery, including CRISPR, is turning potential into impact. In molecular and cellular biology, this happens...Jonathan Gootenberg, PhD, focuses exclusively on this space — developing tools for cellular targeting. Lately...and cleaving RNA at positions defined by the target:spacer duplex without detectable non-specific activity...

...focus. Find yourself a block of time and a quiet space where you can address the issue head on. Prepare...26 ideas, over half of which, when implemented, impacted the entire company. It is important to note that...team has used these techniques to optimize our lab space, our protocols and even our Lab Inventory Management...

...surprised at how this powerful tool is in such a small package. But don’t let the simple presentation deceive ...Transfection of the DNA pooled library and appropriate packaging and envelope plasmids into HEK cells allows production...and composition of your library can drastically impact what conditions it needs to properly amplify.  ...

...routinely use Nanopore technology to sequence our packaged viral DNA for quality control. This gives us a...the issue and resolve the assembly. Keeping up the pace Our QC team constantly faces the challenge of keeping... backend, has enabled the QC to keep up with the pace of deposits while maintaining quality. Maintaining...