We narrowed to 403 results for: Pol;

1. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

   Type

   Blog Post

   ... or a dCas9 fused to the omega subunit of RNA polymerase, the lab   successfully repressed or activated... al., 2014). These could be genes, specific polymorphisms, including antibiotic resistance genes. This...

2. 27 Hot Plasmids from 2016

   Type

   Blog Post

   ...of assembling these multi-protein fusions or “polyproteams”, the Howarth lab created the MBPx-SpyCatcher...sequential additions have been made, the final polyproteam can be eluted using maltose. This procedure allows...

3. Lucky Thirteen: Guest Contributors of 2023

   Type

   Blog Post

   ... Gut to BrainResearchers: Atharva Sahasrabudhe; Polina Aniveeka   A big thank you to all who have contributed...

4. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

   Type

   Blog Post

   ... pcDNA-SADB19L Contains rabies viral RNA polymerase; see depositing paper for more information Edward...

5. DIY DNA Ladders from Penn State University

   Type

   Blog Post

   ...ladder works well for both agarose and native polyacrylamide gels. Get Tips on Verifying Your Plasmid ...

6. Our New Antibody Mascot is....

   Type

   Blog Post

   ...head over to Twitter to vote on @addgene's Twitter poll. Voting is open until Feb. 22, 2022, and we'll unveil...

7. Depositor Week 2020 is Happening Now. Join us!

   Type

   Blog Post

   ...not. We will have Addgene trivia (with prizes!), polls, and shout outs, and we’ll be featuring the work...

8. New Viral Vectors - Summer 2024

   Type

   Blog Post

   ...Jensen New serotype pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA AAV9, PHP.eB Controls Feng New viral service, ...

9. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

   Type

   Blog Post

   ...against a target antigen. This contrasts with polyclonal antibodies, which are mixtures of monoclonal ...

10. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type

    Blog Post

    ...be entirely endotoxin free. Endotoxin is a lipopolysaccharide of the cell wall of Gram negative bacteria...

11. Reflections and Looking Ahead

    Type

    Blog Post

    ...R. A., Virk, H., Laflamme, C., Houston, D. W., Polinski, N. K., Meijers, R., Levey, A. I., Saper, C. B...

12. Top 10 Open Science Developments of 2013

    Type

    Blog Post

    ... February, the Office of Science and Technology Policy issued a memorandum calling on Federal agencies...

13. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type

    Blog Post

    ...these may require restriction fragment length polymorphism analysis, allele-specific PCR, T7EN1 cleavage...

14. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type

    Blog Post

    ...advance COVID-19 research to hopefully help guide policies and ultimately treatments in a timely fashion....

15. 9 Tips to Achieve Success in Academia

    Type

    Blog Post

    ...expertise in law, business, journalism, entertainment, politics, K-12, etc.” Addgene's team includes many PhD ...

16. Antibodies 101: Affinity Tags

    Type

    Blog Post

    ...has you covered! His His tags, often called polyhistidine tags, typically include six consecutive His ...

17. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type

    Blog Post

    ...patterns, post-translational modifications and polymorphisms Codon optimization tool: To access this tool...

18. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type

    Blog Post

    ... with my teammates acted as my safety net and trampoline all at once. I was able to do something that ...

19. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type

    Blog Post

    ...have faster off kinetics and allow neurons to repolarize faster.  Chemogenetics does not offer the same...

20. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type

    Blog Post

    ...molecular biology like restriction enzymes and polymerases. In the situation where you need to use plasmid...