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We narrowed to 988 results for: Ran

Showing: 201 - 220 of 988 results

  1. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ... and 159655 are single guide expressing double stranded break constructs. Plasmids 171098 and 175570 allow...

  3. Serotype Testing AAV

    Type
    Collection
    ... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

  5. Recombinases AAV Preps

    Type
    Collection
    ... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

  8. Chemogenetics AAV Preps

    Type
    Collection
    ... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

  9. Control AAV Preps

    Type
    Collection
    ... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

  10. Optogenetics AAV Preps

    Type
    Collection
    ... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

  11. Biosensor AAV Preps

    Type
    Collection
    ... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

  12. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ... your membrane, run a double-membrane transfer with a psi membrane stacked behind the p membrane. If your... transfer buffer Small proteins: Over-transferring Transfer with two membranes* Psi membrane...alcohol in transfer buffer Mixed proteins: Transferring issues Transfer with two membranes* Electroblot...completely through the membrane or larger proteins not transferring completely onto the membrane. If you’re not...using two membranes during your transfer instead of one. If you see signal on both membranes, you know...large protein, you may be under-transferring.  Membrane pore size Membranes come in different pore sizes ...through the p membrane into the psi membrane, that’s a good indication that the p membrane pores are too...

  13. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...titrating the transposase to transposon ratios. High ratios of transposase to transposon result in a greater...]. Like other transposons, piggyBac™ has two components, a transposon and a transposase. The piggyBac™...piggyBac™ transposase facilitates the integration of the transposon specifically at ‘TTAA’ sites randomly dispersed... piggyBac™ transposon. These plasmids, when combined with a source of piggyBac™ transposase (available...distributor) allow you to quickly transfer a DNA sequence from the transposon vector to one of many TTAA sequences...folks at Transposagen. This post was contributed by Amar Singh and Mathew Goodwin from Transposagen What ...What is the piggyBac™ transposon? The piggyBac™ transposon was originally identified in moths in 1983, but...

  14. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...Cre-dependent transgenes can also be paired with Cre-expressing transgenic mouse lines to restrict transgene expression...with broad CNS or PNS transduction, cell type specificity, or retrograde transport in neurons. (b) Genetic...capsids or transgenes. Sensory neurons and liver appear especially susceptible to off-target transgene expression-induced...It has a similar transduction rate to AAV-PHP.eB in the brain, and a lower transduction rate in the spinal...2022). AAV-PHP.N specifically transduces NeuN+ neurons, with transduction efficiency varying from 10-70%... effectively transduces BALB/cJ, CBA/J, and FVB/NJ mice, with minimal liver transduction observed in both...effectively transduces neurons in the peripheral nervous system (PNS). It has been shown to transduce 80% of...

  15. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ..., and enzymes for transmitter synthesis. Retrograde transport is used for transport of endocytosed material... facilitate transport: retrograde transport relies on dynein, while anterograde transport relies on kinesin...take advantage of existing transport pathways in neurons. Anterograde transport is typically used for the...mechanisms of trans-synaptic trafficking are not fully understood and may be a form of transcytosis where the...after intracellular transport without actually causing infection or leading to transgene expression within...endothelial transcytosis (Foust et al., 2009; Merkel et al., 2017), and these two modes of transport may share...optogenetically controlled transgenes, in combination with conditional transgenic mice, to reveal not only...

  16. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...contains the transposase gene (red) for expressing the transposase enzyme.2.) The transposase enzyme is ...which cuts the DNA.3.) The released transposon can now bind a strand of DNA with a TA dinucleotide (there...removal of the transposon; the plasmid is then degraded by the cell.4.) The transposase creates a double...double strand break in the DNA and allows the tranposon to integrate. Additionally, the TA sequence is duplicated....   The Sleeping Beauty transposon system The Sleeping Beauty (SB) transposon system is one such system...perform gene transfer in vertebrates. The system is comprised of 2 components: 1) an SB transposase, the enzyme...enzyme required for catalysis of transposition; and 2) a transposon containing the gene-expression cassette...

  17. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...piggyBac (PB) transposon: The PB transposon is a mobile genetic element that transposes via a “cut and...Generates transgene-free and vector-free cells Limited genomic integration Multiple transfections required...Delivery DNA-Free High High Transgene-free and vector-free Multiple transfections required Protein Delivery...vector, but transient expression of Cre recombinase allows for deletion of the inserted transgenes that are.... This system is transfected into cells as a donor plasmid containing the transposon, along with a helper...sequences, which the PB transposase recognizes. Following reprogramming, transgenes can be removed by re-...also includes measles and mumps. Sendai transduces a wide range of cell types and replicates in the cytoplasm...

  18. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...forming membrane pores) transfection. These techniques are still used and efficiently transfer plasmid...and stable expression of the transgene   Transfer plasmids The transfer plasmid is the vector you clone...comes to transferring DNA into primary cells or non-dividing cells. To target these un-transfectable cells...Dox), the Tet-controlled transactivator (tTA, also expressed by your transfer vector) binds to the TRE...production and transduction Once you have cloned your gene of interest into lentiviral transfer vector, the...will first need to transfect producing cells, usually 293T cells, with your transfer plasmid and your packaging...you don’t have any experience in transfection, CaCl2 transfection is a simple and cheap technique that...

  19. Technical Design of a Western Blot

    Type
    Blog Post
    ...covers a size range appropriate for the target(s) and transfer method. Membranes The two membrane options ...) steps to the transfer: you’ll need to pre-wet most PVDF membranes before the transfer and allow them...Types of transfers Your transfer options are wet, semi-dry, or dry/electroblot. A wet transfer is the most... while semi-dry transfers require a semi-dry transfer cell. Dry/electroblot transfers only work in the...low-background PVDF membrane, it also has higher background noise than nitrocellulose membranes. Pro tip! You...visualize proteins on a PVDF membrane without staining; simply wet your membrane with 20% methanol and place..., meaning that it will transfer the most protein from your gel to your membrane. It requires anywhere ...

  20. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...variant of PSmOrange, a photoswitchable fluorescent protein which transitions from emitting orange (λ= 565nm...enhancement of transcriptional activation via binding of multiple copies of a transcription factor to a ...the stop codon. Thus a non-translated mRNA will appear yellow, but a translated mRNA will be red. This system... were untranslated in the nucleus, confirming the hypothesis that most mRNAs are not translated until ...P-bodies are translationally repressed whereas the nonsequestered pool goes on to initiate translation. These...tool can also be used to visualize translocations, genome rearrangements and cancer-associated chromosome... been used to study transport characteristics of various Golgi and plasma membrane reporters as well as...
Showing: 201 - 220 of 988 results