... followed soon by GCE done in mammalian cells (Shandell et al., 2021).   How it works If you’re interested...their phosphoserine ncAA protocol, to optimize and share, they’re soliciting feedback from scientists on ...182–188. https://doi.org/10.1126/science.2649980 Shandell, M. A., Tan, Z., & Cornish, V. W. (2021). Genetic...

... navigate when mapping out a new project. We’ve shared some of the concerns that Revolution Bio had to...scientist, and how you were able to overcome them. Share your stories in the comments below! And if you have...developed a genetic tool for plant engineering, please share it with Addgene to both build the plant synthetic...

...are different types of cellulose fibers, pattern, shape. All of that is controlled by regulatory networks...same guide RNA twice. The second problem was the shape of the response function or how the output changes...function of input. Usually that function is in the shape of an S; there is a narrow threshold like a switch...

...powerful in almost any career. It allows you to share and build support for your ideas, invite others ...done a lot of public speaking, and I'm happy to share what I've learned along the way. Own your time ...performance art. You’re (usually) presenting either to share knowledge or to invite critique onto an ongoing ...

...excellent resource for research design is Kalpit Shah et al., “Do you know the sex of your cell?” I can...time—and sex can be hard to determine. To remedy this, Shah et al. provide a table indicating the sex of the...Addgene Blog Learn How Scientists Are Trying to Shape the Future of Research Learn How Notions of Scientific...

...will discuss where better data is needed to help shape and inform the overall structure of the biomedical...area include publishing the book ‘How Economics Shapes Science’ and contributing to the NIH’s Biomedical...PMCID: PMC4000813. 2. McDowell, Gary S., et al. "Shaping the Future of Research: a perspective from junior...

... barcodes within the resistant populations were shared by at least one other replicate; prior to treatment... “I spend a lot of time packing, shipping, and sharing protocols one by one,” and she is excited to deposit...Addgene because it is a “really great way of broadly sharing these kinds of reagents.” Here at Addgene, we’re...

...retrograde transmission (Etessami et al., 2000, Wickersham et al., 2007). Thus, RABV transsynaptic spread...due to lack of G protein on the viral envelope (Wickersham et al., 2007).   Summary and RABV applications...Jun 7;74(5):858-73. Pubmed PMID 22681690.  8, Wickersham IR, Finke S, Conzelmann KK, Callaway EM. Retrograde...

... Since Addgene is very proud to help scientists share plasmids across the globe, we’re working on ways...great lab automation tips and tricks, be sure to share them in the comments section.   Additional Resources... Shannon Rinaldi...

...metabolic pathways. Fertility (F) plasmids: microbial sharing F plasmids contain the genes for bacteria to form...new cloning techniques based on these vectors (Shareck et al., 2004). Plasmid categorization is complex... https://doi.org/10.1016/j.plasmid.2023.102684 Shareck, J., Choi, Y., Lee, B., & Miguez, C. B. (2004)....

...Wormbase. And scientists have been continuously sharing their worm strains through the Caenorhabditis Genetics...C. elegans researchers embrace open science and share resources and tools. That message was fully exemplified...some examples of worm resources and tools openly shared by the worm community. For additional resources...

...difficult-to-engineer Sulfolobus islandicus rod-shaped virus 2 (Mayo-Muñoz et al., 2018). Only viral particles...CRISPR-Based Genome Editing of Sulfolobus islandicus Rod-Shaped Virus 2. Viruses 10:695 . https://doi.org/10.3390...Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR (2016)...

...Many thanks to our guest blogger, Harshana S De Silva Feelixge. Harshana S De Silva Feelixge is a researcher...This post was contributed by guest blogger Harshana S De Silva Feelixge. Gene therapy technologies ...

...experiments involve needles or scalpels (sharps). Cuts and scrapes from sharp equipment are one of the most common... yourself via needlestick or cut. Following all sharps procedures for your organization will significantly...

...engaged in, these issues which will continue to shape the sector throughout their careers (McDowell et...available avenue. Interestingly, both of these changes share a common thread that has the potential to profoundly...PMCID: PMC4866822. 3. McDowell, Gary S., et al. "Shaping the Future of Research: a perspective from junior...

...speak about Addgene’s experience helping scientists share plasmids, especially those of the CRISPR variety...outskirts of Guangzhouon west of beautiful Mao Fengshan Forest Park. As a national science and technology...distinction of “higher” and “lower” life forms as we shared millions of years of common ancestry with worms...

... social media) and freely sharing code, raw data, and more. The team shares results early and often, including...impact,” he says, noting that his team regularly shares troubleshooting tips and works with other labs ...

...? Please share them in the comments below! Special thanks for Dr. Dan Stone and Harshana de Silva ...definitely made my life easier!  In this post, I’ll share some AAV production, purification, and titration...

...concentration of fluorophores) cast shadows along the illumination path. The shadows appear as stripes in the acquired...Klymkowsky. (1989) Development, 105.61-74. Pubmed. J. Sharpe, U. Ahlgren, P. Perry, Hill B, Ross A, J. Hecksher-Sorensen...

...for parents and other members of the community to share their expertise with the kids through some fun activities...Middle school: supplementing the science curriculum Sharing my expertise with our district middle school was... of the talk by running it by the teachers and sharing some slides on Twitter, where I was able to get...