We narrowed to 665 results for: SIM;

...together, thus repairing the break. This is a simplified, streamlined version of this pathway and does...regions of hundreds of base pairs or more can simultaneously introduce a pair of chromosome breaks, and ...deletions) if NHEJ joins the distal ends together. Similarly, it may be possible to direct insertion of an ...

... tools that expand what we can achieve, but are simple to pick up and work well the first time. But biology...functionality to existing genes, while being accessible simply by dialing up the right nucleic acid sequence (...fusion-tolerance — Click Biology tags should be similarly useful across the diverse kinds of proteins that...

...absorb the excitation light less efficiently. Similarly, decreasing the power of the excitation light ...results in a loss of fluorescence, which, in its simplest form, can be described by a mono-exponential decay...intensity decay of a fluorophore does not follow a simple mono-exponential decay (Shaner et al, 2008; Bindels...

...cloning system - a Gateway compatible approach to simultaneously clone two genes-of-interest (GOI) into two ... on a single plasmid (Grefen and Blatt, 2012). Similar to traditional Gateway™ cloning, the GOI first ...the mitochondria, because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder...

...organizations with similar ideas who were also thinking about reproducibility and shared similar desires to take...talented and motivated groups and individuals with similar ideas to actually do something about it. Building...

...rate as compared to a constitutive promoter. To simulate the increase in plasmid copy number during the...expression from the constitutive promoter did not. Similar levels of expression between a plasmid and the ...stabilized promoter, deoxychromoviridans titers were similar regardless of where the genes were expressed. What...

...threonine kinase with a similar 3D structure to MEK1, but Raf1 and CDK5 have less similarity. For these three...versions of MEK1, MEK2, Raf1, and CDK5 all behave similarly to their endogenous counterparts. Additionally...

...by daughter cells during cellular reproduction, similar to the host’s chromosome, or they can spread between...plasmids likely have undiscovered functions, many are simply genetic parasites. Cryptic plasmids tend to be ... R., Sailland, A., Auriol, P., Vogel, T. M., & Simonet, P. (2001). Laboratory-scale evidence for lightning-mediated...

... components. Using the handy website, users can simply select the components they want for each module...license.   Once you have the components you want, simply assemble them into a destination vector via Golden...summary on the Fragmid website.   Step 3 is simply a review of the assembled plasmid. It shows you...

...same target to see if the results are the same/similar, emphasizing the antibody’s specificity. Strength...from the background noise. If the sample images similarly to the negative control, that informs us that ...doi.org/10.1038/518027a Freedman LP, Cockburn IM, Simcoe TS (2015) The Economics of Reproducibility in Preclinical...

...CEPIA (additional versions are available to simultaneously measure calcium changes in the ER, cytosol ...single fluorescent protein (FP), requiring only a simple measurement. However, single wavelength measurements...fluorescence to a red fluorescent state only by simultaneous (1) user-controlled illumination with violet...

... a way to track successfully engineered cells. Similar to transgenic mice, knockout mice are engineered...off-target effects on regions of the genome with similar homology to the site of interest or create mosaic...recombination of floxed sites in the cell can occur. Similar systems include tetracycline or doxycycline mediated...

...secondary structure to induce transcript cleavage, similar to the Rho-independent termination found in prokaryotes...terminate transcription. Polymerase I uses a process similar to the prokaryotic Rho-dependent mechanism, whereas...Definition of the upstream efficiency element of the simian virus 40 late polyadenylation signal by using in...

...perform its helicase and nuclease functions simultaneously, by continuously unraveling dsDNA, pulling ...reeling of ssDNA and unwinding of dsDNA occur simultaneously, which creates a loop in the target strand ...is not as well understood but likely occurs in similar regions. This combination of chewing up one stand...

...biotinylated by BirA. From here, the CAPTURE method is similar to other 3C methods: crosslinking captures chromatin...well-characterized beta globin locus. The two methods had similar rates of specificity for the DNA sequence targeted...multiplexing and high resolution allowed the Xu Lab to simultaneously characterize the ‘social networks’ of 157 super...

...bioengineering, as it has facilitated the development of a simple and scalable means of making targeted genetic edits...for instance, the challenge of attempting to simultaneously and selectively induce both cutting and regulation... targeting a different set of loci to induce simultaneous cutting and transcriptional activation or repression...

... Where publication is expected, the process is similar to academia. The only difference is that your paper...postdocs and do not support or manage them well are simply no longer allowed to have postdocs. In academia...could be laid off while you’re there. Of course, similarly, an academic advisor could also lose their funding...

...also possess similar morphologies, growth properties, and express stem cell markers similar to ESCs [11...11].  While iPSCs and ESCs have similar functional utility, iPSCs bypass the transplantation compatibility...

...the accelerator of a vehicle being stuck down. Similarly, a mutation that turns a tumor suppressor “off...cell to undergo programmed cell death while simultaneously stimulating growth and division. The combined...pathway. Its major appeal is that it enables the simple construction of vectors to study the function of...

... we will mostly use the gamma-retroviruses as a simple spokesmodel for the whole family; however, you ...polymerase gene may seem like a mystery. But it’s simple. The RT protein is made by a separate set of instructions... a tool is done, and the transgenic genome will simply express from whatever promoters were included in...