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We narrowed to 661 results for: SIM;

Showing: 201 - 220 of 661 results

  1. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...rate as compared to a constitutive promoter. To simulate the increase in plasmid copy number during the...expression from the constitutive promoter did not. Similar levels of expression between a plasmid and the ...stabilized promoter, deoxychromoviridans titers were similar regardless of where the genes were expressed. What...

  4. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...threonine kinase with a similar 3D structure to MEK1, but Raf1 and CDK5 have less similarity. For these three...versions of MEK1, MEK2, Raf1, and CDK5 all behave similarly to their endogenous counterparts. Additionally...

  5. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...absorb the excitation light less efficiently. Similarly, decreasing the power of the excitation light ...results in a loss of fluorescence, which, in its simplest form, can be described by a mono-exponential decay...intensity decay of a fluorophore does not follow a simple mono-exponential decay (Shaner et al, 2008; Bindels...

  6. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...by daughter cells during cellular reproduction, similar to the host’s chromosome, or they can spread between...plasmids likely have undiscovered functions, many are simply genetic parasites. Cryptic plasmids tend to be ... R., Sailland, A., Auriol, P., Vogel, T. M., & Simonet, P. (2001). Laboratory-scale evidence for lightning-mediated...

  7. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ... components. Using the handy website, users can simply select the components they want for each module...license.   Once you have the components you want, simply assemble them into a destination vector via Golden...summary on the Fragmid website.   Step 3 is simply a review of the assembled plasmid. It shows you...

  8. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...same target to see if the results are the same/similar, emphasizing the antibody’s specificity. Strength...from the background noise. If the sample images similarly to the negative control, that informs us that ...doi.org/10.1038/518027a Freedman LP, Cockburn IM, Simcoe TS (2015) The Economics of Reproducibility in Preclinical...

  9. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ... a way to track successfully engineered cells. Similar to transgenic mice, knockout mice are engineered...off-target effects on regions of the genome with similar homology to the site of interest or create mosaic...recombination of floxed sites in the cell can occur. Similar systems include tetracycline or doxycycline mediated...

  10. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...CEPIA (additional versions are available to simultaneously measure calcium changes in the ER, cytosol ...single fluorescent protein (FP), requiring only a simple measurement. However, single wavelength measurements...fluorescence to a red fluorescent state only by simultaneous (1) user-controlled illumination with violet...

  11. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...secondary structure to induce transcript cleavage, similar to the Rho-independent termination found in prokaryotes...terminate transcription. Polymerase I uses a process similar to the prokaryotic Rho-dependent mechanism, whereas...Definition of the upstream efficiency element of the simian virus 40 late polyadenylation signal by using in...

  12. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...perform its helicase and nuclease functions simultaneously, by continuously unraveling dsDNA, pulling ...reeling of ssDNA and unwinding of dsDNA occur simultaneously, which creates a loop in the target strand ...is not as well understood but likely occurs in similar regions. This combination of chewing up one stand...

  13. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...biotinylated by BirA. From here, the CAPTURE method is similar to other 3C methods: crosslinking captures chromatin...well-characterized beta globin locus. The two methods had similar rates of specificity for the DNA sequence targeted...multiplexing and high resolution allowed the Xu Lab to simultaneously characterize the ‘social networks’ of 157 super...

  14. Academic vs. Industry Postdocs

    Type
    Blog Post
    ... Where publication is expected, the process is similar to academia. The only difference is that your paper...postdocs and do not support or manage them well are simply no longer allowed to have postdocs. In academia...could be laid off while you’re there. Of course, similarly, an academic advisor could also lose their funding...

  15. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...bioengineering, as it has facilitated the development of a simple and scalable means of making targeted genetic edits...for instance, the challenge of attempting to simultaneously and selectively induce both cutting and regulation... targeting a different set of loci to induce simultaneous cutting and transcriptional activation or repression...

  17. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...the accelerator of a vehicle being stuck down. Similarly, a mutation that turns a tumor suppressor “off...cell to undergo programmed cell death while simultaneously stimulating growth and division. The combined...pathway. Its major appeal is that it enables the simple construction of vectors to study the function of...

  18. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...Nanobodies can be labeled with up to 3 dyes, yielding similar signal strength to a standard IgG secondary antibody...two-step staining with antibodies. Nanobodies also simplify multicolor staining experiments because they don...with this multi-color staining workflow yields similar localization patterns as cells stained for one ...

  19. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...and I guarantee you have developed them through similar experiences in graduate school. These skills will...they use, you’ve gained experience working with similar protocols, and look - they’re seeking someone who...strength until it is identified by another person. Similar reasoning may also apply to other acquired skills...

  20. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ... we will mostly use the gamma-retroviruses as a simple spokesmodel for the whole family; however, you ...polymerase gene may seem like a mystery. But it’s simple. The RT protein is made by a separate set of instructions... a tool is done, and the transgenic genome will simply express from whatever promoters were included in...
Showing: 201 - 220 of 661 results