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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 38 results for: SIM;

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Showing: 1 - 20 of 38 results

  1. Genetic Code Expansion

    Type
    Collection
    ...Mammalian TAG Simon Elsaesser 140011 pAS_4xMx PylT_FLAG-Mx PylRS PylRS M. alvus CpK Mammalian TAG Simon Elsaesser... TAG Simon Elsaesser 140013 pAS_4xMx PylT C41CA_FLAG_Mx PylRS PylRS M. alvus Mammalian TAG Simon Elsaesser...TAG Simon Elsaesser 140023 pAS_4xMma PylT_FLAG-Mma PylRS AF PylRS AF M. mazei Mammalian TAG Simon Elsaesser... TAG Simon Elsaesser 154763 pAS_FLAG-G1 PylRS G1 PylRS Methanogenic archaeon Mammalian TAG Simon Elsaesser...Mammalian TAG Simon Elsaesser 174896 pAS_4xMmaPylT(UUA)_EF1_MmaPylRS PylRS M. mazei Mammalian TAA Simon Elsaesser...Mammalian TGA Simon Elsaesser 174901 pAS_MmaPylT_EF1_NES-MmaPylRS WT PylRS M. mazei Mammalian TAG Simon Elsaesser...C41CA_FLAG_Mx PylRS YA PylRS M. alvus Mammalian TAG Simon Elsaesser 140018 pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG...

  2. CRISPR Guide

    Type
    Collection
    ... every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most... change the genomic target of the Cas enzyme by simply changing the target sequence present in the gRNA...The requirements for a CRISPR knockout assay are simple. You’ll need a Cas enzyme and a gRNA specific to...AcrVA3.1, which inhibits both Cas12a and Cas3. Similarly, some Acr proteins are specific to a particular...editors are based on cytosine base editors and similarly work by first converting cytidine to uridine. ...repress gene expression in mice and human cells. The simplest dCas9-based activators and repressors consist ...imaging has numerous advantages, including the simplicity of gRNA design, programmability for different...

  3. Tetracycline Inducible Expression

    Type
    Collection
    ... VP16 transcriptional activation domain). For simplicity, other promoter elements and transcription machinery... TetR with the activation domain of the herpes simplex virus VP16 protein (Gossen and Bujard, 1992). This...dox-inducible system). The outcome of Tet-On is similar to the natural TetR-tetO system, even though the... downstream gene via its activation domain than simply removing a repressor. Tet-On has since become one...Pooled Library Lentiviral barcode library for simultaneous tracing of clonal lineages and transcriptional...

  4. Antibody Guide

    Type
    Collection
    ...assays that have multiple proteins to detect simultaneously. Conjugation The process of attaching signaling...conjugate or looking for antibodies validated for a similar assay type) and search again. After filtering, ..., directly compare the product information for similar antibodies, looking for ones that have identical...price, shipping availability, ease of purchase, or similar factors. Secondary antibodies Secondary antibodies...

  5. Retrograde AAV viral preps

    Type
    Collection
    ...pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression with simultaneous dTomato expression Recombinases Rylan Larsen...CaMKII-somBiPOLES-mCerulean CamKII Bidirectional Optogenetics Simon Wiegert 154951 hSyn-DIO-somBiPOLES-mCerulean Syn...Syn Bidirectional, Cre-dependent Optogenetics Simon Wiegert 44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Syn Activator...

  7. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...sequences can be designed using Benchling or using similar design tools and ordered as standard desalted oligonucleotides...A., Park, K. U., & Brzezinski, J. A. (2020). Simultaneous deletion of Prdm1 and Vsx2 enhancers in the ...

  8. Mammalian RNAi Tools

    Type
    Collection
    ...cleavage of the target. RNAi using siRNA follows a similar mechanism. Since it is pre-processed, it can bypass...., & Nemunaitis, J. (2009). siRNA vs. shRNA: Similarities and differences . Advanced Drug Delivery Reviews...

  9. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Allows for visualization of multiple proteins simultaneously with dual labeling when used with two substrates...by ACP Synthase, thereby permitting selective simultaneous labeling in a sample. Please see the note below...

  10. TALEN Guide

    Type
    Collection
    ...target sites. (B) A TAL effector nuclease (TALEN). Similar in principle to the ZF nuclease, the components...with ZF arrays, if not slightly lower. Figure 2: Simplified representation of the Voytas/Bogdanove Golden...

  11. Plant Plasmids and Resources

    Type
    Collection
    ...constructing CRISPR gene-editing vectors in just two simple cloning steps. CRISPR Kate Creasey Krainer , Vladimir...system based on tobacco rattle virus (TRV) that simplifies gene silencing and genome editing logistics. ...

  12. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Cas9 vector based on the Zhang lab's PX330 , simultaneously targeting the genomic site and the donor vector...Degron Tagging Masato Kanemaki's lab has developed a simple and scalable CRISPR/Cas-based method to tag endogenous...

  13. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... and tyrosine recombinases, based on sequence similarity and the amino acid residue used in the active...to turn off the expression of one gene while simultaneously turning on another. The FLEx switch system ...

  14. CRISPR References and Information

    Type
    Collection
    ...gene, as well as a target site common to a set of similar sequences. Organisms include human, mouse, rat...for NmCas9 and BsmBI site for cloning in gRNA: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF, 49...

  15. Optogenetics AAV Preps

    Type
    Collection
    ...soma-targeted) mCerulean Constitutive 5, 9, rg* Simon Wiegert 154951 hSyn-DIO-somBiPOLES-mCerulean Syn...soma-targeted) mCerulean Cre dependent 5, 9, rg* Simon Wiegert No available items found to match all requested...

  16. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...based on shared characteristics and evolutionary similarity. These are grouped into two large classes based...conditions. Genome editing with CRISPR is not only simple, but also scalable. Multiple gRNAs targeting multiple...

  17. Zhang Lab CRISPR Page

    Type
    Collection
    ...-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran et al., Nature Protocols...cancer modeling Using Cas9 mice, Platt et al . simultaneously modeled the dynamics of KRAS , p53 and LKB1...

  18. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...non-specific labeling observed Publications Ben-Simon, Y., Hooper, M., Narayan, S., Daigle, T. L., Dwivedi...Bishaw, Y. M., Bendrick, J. L., Gore, B. B., Ben-Simon, Y., Opitz-Araya, X., Martinez, R. A., Way, S. W...

  19. Immunology Research Plasmids and Resources

    Type
    Collection
    ...with sequence similarity 3, member C GS3786, ILEI FAM3D family with sequence similarity 3, member D EF7...FLJ16023, KIAA0832, NR3B3 FAM3B family with sequence similarity 3, member B 2-21, C21orf11, C21orf76, ORF9, PANDER...platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) FLJ12858...leukocyte protein of 76kDa) SLP-76, SLP76 LOC652578 similar to Fc fragment of IgG, low affinity IIIa, receptor...

  20. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Zap) New alternately colored FRET sensors for simultaneous monitoring of Zn 2+ in multiple cellular locations...detect subthreshold membrane potential changes Simultaneous two-photon imaging of action potentials and ...
Showing: 1 - 20 of 38 results