We narrowed to 303 results for: SPI;

1. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   ...dish containing the circled clones is removed by aspiration and the dish left covered (to avoid drying out...

2. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

   Type

   Blog Post

   ...Editing of Pten by CRISPR/Cas9 in Mouse Liver in Spite of Cas9-Specific Immune Responses. Human Gene Therapy...

3. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

   Type

   Blog Post

   ...postdoctoral training from Massachusetts General Hospital and Harvard Medical School. Connect with her on...

4. Truncated gRNAs for Regulating Gene Expression

   Type

   Blog Post

   ...researchers at the Wyss Institute for Biologically Inspired Engineering. CRISPR/Cas9 technology has revolutionized...

5. Plasmids 101: Knockout/Knock-In Plasmids

   Type

   Blog Post

   ... inactivate specific genes in mammalian cells. Despite the popularity of CRISPR-based knockout/knock-in...

6. Plasmids 101: Optimizing Plasmid Yields

   Type

   Blog Post

   ..., or a home-brew optimized for plasmid yields. Spiking your media with supplements such as magnesium salts...

7. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

   Type

   Blog Post

   ...flow-through solutions and use clean tubes? How about spin times, were these long enough?  There are, of course...

8. Antibodies 101: Producing Recombinant Antibodies

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   Blog Post

   ...Brechmann et al. al., 2021) while the media is aspirated. The beads are then washed, followed by antibody...

9. An Introduction to Adenovirus

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   Blog Post

   ...sense of what AdVs are about – and we hope that it inspires further use of existing vectors, as well as research...

10. Course-Based Undergraduate Research Experiences (CUREs)

    Type

    Blog Post

    ...Kimball et al., 2020). Benefits and Challenges CUREs inspire students to take ownership of their work and become...

11. Uncovering Ancestral Bias in CRISPR Research

    Type

    Blog Post

    ...'s Genetic Perturbation Platform to modify the CRISpick algorithm to prevent guides from being designed...

12. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    ...gene-transfer tools in the study of the brain and spinal cord, which together are known as the central nervous...

13. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    ...Vanderperre S, Paul R, Björklund M, Ahr D, Ahmed AW, Spinelli L, Brun C, Basler K, Merabet S (2018) Generation...

14. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    ... the U.S. (42%), Europe (23%), and China (14%). Spikes in plasmid requests correspond to publications ...

15. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    ...begin and end on time? Did the conversation remain spirited and focused as opposed to a wandering monologue...

16. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    ...Flame is more than an award for depositors and inspiration for our updated mascot, it’s also a convenient...

17. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type

    Blog Post

    ...Karl-Klaus Conzelmann. "Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is...

18. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    ...that extra two miles which is way more fun. And despite those who may wish to tell you so – towel corners...

19. Twenty Years of Sharing: Expanding Accessibility

    Type

    Blog Post

    ...shipping. However, there were issues that persisted despite our best efforts. With a little over half of our...

20. Plasmids 101: Stringent Regulation of Replication

    Type

    Blog Post

    ...Solar, G., Giraldo, R., Ruiz-Echevarría, M. J., Espinosa, M., & Díaz-Orejas, R. (1998). Replication and...