...digital PCR, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector ...

...of powder into 100 mL of deionized water. While stirring, slowly add hydrochloric acid until the solution...

...time by shortening or skipping many steps and will still get enough colonies for your next step. Remember...

...Remove the paraformaldehyde and follow your institution's laboratory safety guidelines for disposing of...

...release the plunger, allowing it to go back to its resting position. Wait for the liquid to stop flowing into...

...phase Middle phase - A white layer may appear, consisting of precipitated protein particles Bottom phase...

...lyophilized powder. The researcher then needs to reconstitute their primers in liquid, normally sterile dH...

...Insert Vector Type PI Publication Do you have suggestions for other plasmids that should be added to this...

... May 2014 Vol. 15 No. 5, p. 3626 Do you have suggestions for other plasmids that should be added to this...

...asks questions, calling on 1-2 panelist for each question in a way that elicits those interesting stories...good questions with trusted audience members. A good first question is a model for the questions that ...You know, the one where the questions are obvious, the answers uninteresting, the panel members all say... no time for audience questions and an audience member asks a 5 minute question that is really a speech...list of questions for the actual event, based on these interviews. The idea is to ask questions in a directed...spontaneity, but having a good handle on some interesting questions and topics in advance is key. Make sure ... the panelists answer all the questions (they won't all have interesting or new answers). Know when to...

...wages, overtime tracking? What are institutions doing? Institutions are now preparing to comply with the...resource on what institutions are actually doing (using the list of 340 institutions with postdocs in .... Most institutions suggest that postdocs should be paid according to the National Institutes of Health...In 2014, 89% of institutions had a minimum salary policy: of those, 51% of institutions set their postdoctoral...should be on the lookout for what their institutions, or institutions they're thinking about applying to, ...salaries will have to double at some institutions; and that institutions which can more easily provide bridge... level (the current average postdoc salary is estimated at around $45,000, but as I’ve discussed elsewhere...

...were at institutions focused only on tracking hours, and 22% of postdocs were at institutions where no..., while the mandate has been removed, institutions should still go ahead with raises, as for years there... of some public institutions) to cancel plans to raise salaries. What are institutions doing after the...interested in whether institutions planning to raise salaries prior to the injunction are still honoring these...to the injunction still won’t after the injunction (see below). Of the institutions who were planning...planning unionization at their institutions. One clear message that institutions have sent, with the lack of...their institution is doing from Future of Research over social media, not from their institution), is ...

...purification based on the polyhistidine tag. What is the polyhistidine tag? The polyhistidine tag is a string of....      Fig 2:  A, The polyhistidine tag is a string of histidine amino acids on the N- or C- terminus...residues Deciding how many histidine residues to use is a bit easier. The more histidine residues that are present... low level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended... this blog has answered all of your questions about polyhistidine tags. For more antibody-related information... of 6 or more histidine residues expressed in frame on the N- or C-terminus of a recombinant protein of...based on the affinity of the imidazole ring of histidine for metal ions such as Ni2+ or Co2+ (Hochuli 1988...

...field. It is a mechanistic problem, a "how" question, not a vague goal or a "what" question. For example... ambitious goals and can help you distinguish mechanistic questions from open ended screens and data collection...terms of the question, the methods, the tools or techniques that can help resolve the question, the anticipated...full chalk talk with questions from the committee. For a list of common questions, check out this eBook...Committee questions can derail your talk. You need to balance respectfully answering questions while steering... Research Campus of the Howard Hughes Medical Institute. Are you currently on or planning to go on the... 45 minutes. The faculty will ask you lots of questions. Your task is to convince them to accept you as...

..., certain technical considerations still apply. Testing stimulation First, In lieu of electrophysiology...that are distinguished visually through different patterns and the animal only receives stimulation in one...actually stimulating or inhibiting the targeted neuronal population prior to behavioral testing. One method...receive different types of stimulation may help to refine and optimize your stimulation parameters as well. ... and stimulate them for 30min to an hour and observe what happens. You never know how stimulation of a...personally interested in include: intracranial self-stimulation (ICSS) and place preference. The lab I work in... electrode and non-specifically electrically stimulating an ill-defined region of the brain. With the ...

...those in eukaryotic cells. Some promoters are constitutively active and on all the time while others are...the cell, promoters themselves are controlled by still other regulatory factors: enhancers, boundary elements...prokaryotes We have included two reference tables below listing some of the most common bacterial and mammalian...mammalian promoters. These lists is by no means exhaustive, but should be a good place to start when trying to...expression promoter from the human cytomegalovirus Constitutive  May contain an enhancer region. Can be silenced...expression from human elongation factor 1 alpha Constitutive  Tends to give consistent expression regardless...promoter from the simian vacuolating virus 40 Constitutive  May include an enhancer. PGK1 (human or mouse...

...going to get. There are questions that are answerable in minutes and questions that could take weeks to...combatitive. Not every question is answerable, particularly when it comes to technical questions. BUT, I do believe... Double check to make sure the answer to your question didn’t come with your product - this could save...right on the tube itself! Is the answer to your question on your product’s web page, or in the company’...probably stop using that company). Frequently asked question pages on website are another great source of information... information. At least at Addgene, the questions in our FAQ section are really, truly frequently asked...skeptical about using Google for serious science questions, consider using Google Scholar, which searches...

...his eBook, adds: "If you have any questions, comments or suggestions, please do not hesitate to email ...process to distill a one-size-fits-all recipe for success. What I can promise is that my suggestions can help... people in your field that still need to understand your specific questions and experiments. Nobody EVER...aims of a chalk talk. Questions take a lot of time to discuss and the questions are important. However...It helps to come from the lab of a well-known investigator. If you only have middle author papers or no...It's much more impressive to answer an expert's question than to leave everyone confused about your research... The chalk talk, simple in principle The first question I usually get about chalk talks is, what is a ...

...COVID-19 has caused an estimated total of 380,000 deaths worldwide (statistics provided by Google on 6... Rough estimation of COVID-19 effect on plasmid requests Before we began any sophisticated analyses,...COVID-19 cases each week. Statistical Modeling Note -- If statistical modeling isn’t up your alley,...region using stochastic gradient descent in Pytorch, which gave us the maximum likelihood estimates of each...Director of the Merkin Institute and Vice-Chair of the Faculty at the Broad Institute; Professor of Chemistry...Harvard University; and Howard Hughes Medical Institute Investigator. Liu’s research integrates chemistry and...Harvard University, and David R. Liu from the Broad Institute and Harvard University. Over the course of the...

...hours for answering questions. She was able to do this because we could see the questions being posted as ...mechanics of doing the AMA were interesting. I started watching the questions and preparing answers early ... professional development etc. database. Still have questions? I am easy to reach and happy to help. ...Reddit community is active, engaged, and has great questions! 2. The r/Science subreddit is well moderated....facilitated scheduling, filtered out inflammatory questions, and even coached us on how to write our AMA submission...and Joanne’s preferred time to start answering questions. r/Science has pretty strict rules about not being...prepare answers beforehand. Joanne answered many questions in the AMA but, as you’ll learn below, even with...