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Addgene

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We narrowed to 712 results for: STO

Showing: 201 - 220 of 712 results

  1. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    ...Stanford develops engineered DNA systems capable of storing data and computing inside living cells. He's also...describing the development of “transcriptors,” transistor-like digital genetic switches that enable cellular...the beginnings of computational logic and data storage. What we don't have are the keyboards and displays...mining. There is an absolutely incredible hidden story here. The parts we are releasing simply plug into...

  2. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...unevenly, introducing distortions in your final images. It is possible to keep distortions to a minimum with...done about that.  Second, expansion introduces distortions. The goal in expansion is for your sample to ... L (2022) Super-resolution microscopy: a brief history and new avenues. Philosophical Transactions Royal...

  3. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ... convey enough information to tell a compelling story in an image or do you need a full exhibit? In a ....  Static. Can be difficult to convey an entire story or context via still images. Some things cannot ...information. Primarily useful for data-focused stories. In-person lectures, presentations, etc… Low...interested in scicomm. Difficult to do in-depth storytelling. Requires regular content production. Internet...

  4. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...Biomedical research has a long and well-documented history of racial bias, which still impacts and influences...ancestry.  Understanding the impact Fig. 3: Histograms indicating the frequency (x-axis) which SNPs ...ancestry-based bias.   The correction process does not stop there. Currently, they are working with Broad's ...Further complicating the issue is the long and ugly history of reporting false essentialist links between biology...

  5. Technical Design of a Western Blot

    Type
    Blog Post
    ...like rotting eggs but has a longer shelf life when stored properly. Because BME is not as strong as DTT, ..., while expensive, reduce protocol time, can be stored at 4 °C, and are very consistent gel-to-gel.  Ladder...multiple times. It doesn’t degrade during long-term storage and has both high binding capacity and high protein...inexpensive and easily made in the lab. They can be stored at your bench during the procedure and at 4 °C ...Fluorescent proteins are stable, so blots can be stored for weeks to months without loss of signal. While...

  6. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...diminished KLF4 expression levels; this altered stoichiometry impacted reprogramming and global gene expression...redshifted-emission spectrum compared to that of CyOFP1, a large stokes shift, long fluorescence lifetime, and a stable...affecting many of the RFPs developed from natural ancestors), and screened through libraries of new RFP variants... an FP’s properties. pBAD-LSSmCherry1 is a long Stokes shift variant, which could be compatible with two-photon...entire protein-coding regions (excluding start and stop codons) with kanamycin cassettes and were carefully...

  7. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ... done at my first six ASM meetings. I told this story over a great dinner at Pittypat’s Porch with old... career scientists, students, and those from historically underrepresented groups. Through such mentoring...Dave Baltrus, Geof Hannigan, Irene Newton, Juliet Johnston, Kat Milligan-Myhre, Kim Dill-McFarland, Lisa ...never went away. While he has frequent bouts with Impostor Syndrome, Joe has found his people through meetings...

  8. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ... payoff for each aim. Learn to communicate your story with only the most salient details. Make the most...your research problem. Creating a coherent unified story and model can help make your ideas easier to understand...Practice presenting your chalk talk Perfect your story and delivery. Design simple figures and diagrams...track. Furthermore, practice getting back to your story. Committee questions can derail your talk. You need...

  9. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...protein of interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific antibodies...technologies such as array tomography, super-resolution STORM imaging, and electron microscopy. Sample preparation...standard proteins tags like GFP. Specifically for STORM, the high intensity signal provided by smFPs can...Rizzo, Mark A., Michael W. Davidson, and David W. Piston. "Fluorescent protein tracking and detection: fluorescent...

  10. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...infectious titer. The infectious titer of a viral vector stock can vary based on transduction target and can also...particles were full, compared to 50% of wild-type AAV2 stocks (Grimm et al., 1999). The percentage of genome-...100nm. Image courtesy of David Bell and Svetla Stoilova-McPhie, Center for Nanoscale Systems, Harvard ...Quantitative PCR (qPCR) Digital droplet PCR (ddPCR) Historically, a quantitative DNA hybridization method (DNA...

  11. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...single protein, shedding the complexity of its ancestors. They classified the system as Class I, Type III-E...editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development and discovery The ...editing treatment would have to be individually customized to the patient's specific mutations. With a drag...Kaiyi Jiang, Nathaniel Roberts, Liyang Zhang, Christopher A. Vakulskas, John A. Walker II, Anastasia P....

  12. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...below. The GUS gene (β-glucuronidase) with histochemical GUS-staining is a very useful visual reporter...to inject an Agrobacterium culture through the stomata into the abaxial site (lower surface) of the leaf...MoClo system you can easily and quickly create customized vectors. For more information on MoClo for plants...is a PhD candidate at Northeastern University in Boston, MA. He obtained his BSc and MSc in Plant Biotechnology...

  13. How to Write a Scientific Review Article

    Type
    Blog Post
    ...Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell development...scientist’s tool kit. I wrote a review article on histone modifers in B and T cells with my adviser when ... sentences, make it flow, and start telling the story. Like any editing process, you will need time away...

  14. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...interactions in living cells using a microscope. Historically, molecular interactions were detected by indirect...brightness of a fluorophore pair, donor:acceptor stoichiometry, and cross-talk between the two fluorophore ...has five-fold less brightness than YFP. The stoichiometry of donor:acceptor is difficult to address especially... Transfer    Interesting Articles About FRET: Piston DW, Kremers GJ. Fluorescent protein FRET: the good...

  15. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...key to fitting large genes into AAV First, some history. In 2008, the Auricchio lab decided to solve the..., the partial genomes complement each other to restore the full-expression cassette. AAV’s natural ability...truncated protein. This can be avoided by excluding a stop codon from donor A in any of the reading frames ...the gRNA target site so that transcripts undergo nonstop decay (Frischmeyer et al., 2002). How to pick ...

  16. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...multiple exons that, when deleted through NHEJ, will restore the dystrophin reading frame. gRNAs bind in intronic... blot, at <10% of the wild-type level. Muscle histology is improved, with vastly reduced inflammation ...this result, Nelson et al. find that dystrophin restoration is maintained for at least six months. Future..., Olson EN. Postnatal genome editing partially restores dystrophin expression in a mouse model of muscular...

  17. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ... translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes a mutant...OFP (orange), is expressed when Cre is absent; stochastic Cre excision results in one of three recombinations...anemone mKate2.) They then successfully generated custom antibodies to each of these proteins and confirmed...

  18. A Control for All Seasons

    Type
    Blog Post
    ...Stephen M., et al. “Controls for Immunohistochemistry.” Journal of Histochemistry & Cytochemistry, vol. ...Controls for Immunocytochemistry.” Journal of Histochemistry & Cytochemistry, vol. 59, no. 1, SAGE Publications...and Practicalities of Immunoblotting and Immunohistochemistry: A Guide for Submission to the British Journal...

  19. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...MasScience, ECUSA, IMFAHE and Bostonadas. Follow her on twitter @Bostonadas.         Rocío López-Diego MD...relationship for the mentee, where (s)he feels truly understood and supported, and can more fully identify with...University, Massachusetts Institute of Technology, Boston University, Johns Hopkins University, the Salk ...

  20. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...undergraduate years. We each have a unique “origin story,” but taking the first step into the world of academic... to find out what a “postdoc” is, and that “PI” stood for principal investigator. In basic science (as...after earning a doctorate Considered a "stepping stone" position to become a professor, or other scientific...the value in finding mentors wherever I go. This story by Dr. Erika Moore in Science Careers discusses ...
Showing: 201 - 220 of 712 results