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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 424 results for: SUA

Showing: 201 - 220 of 424 results

  1. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...the key to a great image is contrast. The human visual system relies primarily on contrast to identify... is collected and focused onto a camera for visualization. Illuminating a sample in the opposite direction...

  3. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...Grade DNA (high quality/low quantity) – You can usually complete a cloning experiment with a relatively...you flow lysate and buffers through the matrix, usually by centrifugation or vacuum. In a bead-based approach...

  5. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...In all cases, structural biology can help you visualize the system you’re working with and better understand...developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California...

  6. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...by testing them. For intracellular targets, we usually test 3 different fix/perms (methanol, PFA-saponin...lines using these two antibodies. This is the most usual level of evidence supplied to support the suitability...

  9. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...remove virus from cells Plasmid Non-integrating, usually Medium Medium Generates transgene-free and vector-free...lower levels of efficiency at generating iPSCs, usually several orders of magnitude lower than retroviruses...

  10. Deep Dive: qPCR

    Type
    Blog Post
    ...samples, and treatments may affect the assay. It’s unusual to need to adjust amounts of reagents in your chemistry...reference genes deserves more attention than it usually receives. Different reference genes have shown ...

  13. Ligation Independent Cloning

    Type
    Protocol
    ...base-pairing in the short overlapping regions (usually 4 bp) does not provide enough stability to hold...

  14. Video Library

    Type
    Protocol
    ...laboratory procedure for separating DNA by size for visualization and purification. Agarose Gel Electrophoresis...

  15. Colony Formation Titering Assay

    Type
    Protocol
    ...the dilutions well Note: the 1:10 dilution can usually be omitted because this dilution typically produces...

  16. Antibody Guide

    Type
    Guide
    ...Chromatin immunoprecipitation (ChIP) Visualization - used to visualize protein location. Includes: Immunofluorescence...immunoprecipitation. Antibody Applications - Visualization Methods Visualization methods such as immunofluorescence...individual assay conditions. Controls for visualization methods Visualization methods should employ positive, negative...Specificity Other Affinity Reagents Handling Visualization Signal Amplification Application Overview Quantification...Quantification Methods Capture Methods Visualization Methods Cell Sorting Methods Multiplex Assays Selection...accessible to antibodies. Detection of sdAbs is usually achieved by conjugation of a signaling molecule...before conjugating your antibodies. Antibody Visualization The ability of an antibody to specifically bind...

  19. Western Blot

    Type
    Protocol
    ...video to learn how to use western blotting to visualize a protein from cells or tissue samples. Equipment...

  20. CRISPR Guide

    Type
    Guide
    ...Overview of visualizing genomic loci with fluorophores using CRISPR Browse Plasmids: Visualize Purify Genomic...unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high on-target... become a flexible tool to activate, repress, visualize, and isolate genes. Activation or Repression of...modification using CRISPR Browse Plasmids: Epigenetics Visualizing Genomic Loci Using Fluorophores CRISPR imaging...multiple targets simultaneously. CRISPR imaging can visualize chromatin and RNA dynamics in live cells. Labeling... painting, and RNA tracking in living cells. Visualizing a specific genomic locus requires recruitment...Chromosome-specific repetitive loci can be efficiently visualized in living cells using a single gRNA that has ...
Showing: 201 - 220 of 424 results