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  2. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...In all cases, structural biology can help you visualize the system you’re working with and better understand...developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California...

  4. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...by testing them. For intracellular targets, we usually test 3 different fix/perms (methanol, PFA-saponin...lines using these two antibodies. This is the most usual level of evidence supplied to support the suitability...

  7. Deep Dive: qPCR

    Type
    Blog Post
    ...samples, and treatments may affect the assay. It’s unusual to need to adjust amounts of reagents in your chemistry...reference genes deserves more attention than it usually receives. Different reference genes have shown ...

  8. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...remove virus from cells Plasmid Non-integrating, usually Medium Medium Generates transgene-free and vector-free...lower levels of efficiency at generating iPSCs, usually several orders of magnitude lower than retroviruses...

  9. Cre-lox system

    Type
    Collection
    ...fusion of Cre to a fluorescent reporter enables visualization of Cre expression. Optimized Cre: Codon-optimized...

  11. Ligation Independent Cloning

    Type
    Protocol
    ...base-pairing in the short overlapping regions (usually 4 bp) does not provide enough stability to hold...

  12. Video Library

    Type
    Protocol
    ...laboratory procedure for separating DNA by size for visualization and purification. Agarose Gel Electrophoresis...

  13. Colony Formation Titering Assay

    Type
    Protocol
    ...the dilutions well Note: the 1:10 dilution can usually be omitted because this dilution typically produces...

  16. Western Blot

    Type
    Protocol
    ...video to learn how to use western blotting to visualize a protein from cells or tissue samples. Equipment...

  17. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ... look at various CRISPR applications (such as visualize, screen, activate, and repress) on Thursday. We...
Showing: 201 - 220 of 410 results