Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 420 results for: SUA

Showing: 201 - 220 of 420 results

  1. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...Grade DNA (high quality/low quantity) – You can usually complete a cloning experiment with a relatively...you flow lysate and buffers through the matrix, usually by centrifugation or vacuum. In a bead-based approach...

  3. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...by testing them. For intracellular targets, we usually test 3 different fix/perms (methanol, PFA-saponin...lines using these two antibodies. This is the most usual level of evidence supplied to support the suitability...

  6. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...remove virus from cells Plasmid Non-integrating, usually Medium Medium Generates transgene-free and vector-free...lower levels of efficiency at generating iPSCs, usually several orders of magnitude lower than retroviruses...

  7. Deep Dive: qPCR

    Type
    Blog Post
    ...samples, and treatments may affect the assay. It’s unusual to need to adjust amounts of reagents in your chemistry...reference genes deserves more attention than it usually receives. Different reference genes have shown ...

  10. Ligation Independent Cloning

    Type
    Protocol
    ...base-pairing in the short overlapping regions (usually 4 bp) does not provide enough stability to hold...

  11. Video Library

    Type
    Protocol
    ...laboratory procedure for separating DNA by size for visualization and purification. Agarose Gel Electrophoresis...

  12. Cre-lox system

    Type
    Collection
    ...fusion of Cre to a fluorescent reporter enables visualization of Cre expression. Optimized Cre: Codon-optimized...

  13. Colony Formation Titering Assay

    Type
    Protocol
    ...the dilutions well Note: the 1:10 dilution can usually be omitted because this dilution typically produces...

  16. Western Blot

    Type
    Protocol
    ...video to learn how to use western blotting to visualize a protein from cells or tissue samples. Equipment...

  17. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ... look at various CRISPR applications (such as visualize, screen, activate, and repress) on Thursday. We...
Showing: 201 - 220 of 420 results