...Chromatin immunoprecipitation (ChIP) Visualization - used to visualize protein location. Includes: Immunofluorescence...immunoprecipitation. Antibody Applications - Visualization Methods Visualization methods such as immunofluorescence...individual assay conditions. Controls for visualization methods Visualization methods should employ positive, negative...Specificity Other Affinity Reagents Handling Visualization Signal Amplification Application Overview Quantification...Quantification Methods Capture Methods Visualization Methods Cell Sorting Methods Multiplex Assays Selection...accessible to antibodies. Detection of sdAbs is usually achieved by conjugation of a signaling molecule...before conjugating your antibodies. Antibody Visualization The ability of an antibody to specifically bind...

...Overview of visualizing genomic loci with fluorophores using CRISPR Browse Plasmids: Visualize Purify Genomic...unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high on-target... become a flexible tool to activate, repress, visualize, and isolate genes. Activation or Repression of...modification using CRISPR Browse Plasmids: Epigenetics Visualizing Genomic Loci Using Fluorophores CRISPR imaging...multiple targets simultaneously. CRISPR imaging can visualize chromatin and RNA dynamics in live cells. Labeling... painting, and RNA tracking in living cells. Visualizing a specific genomic locus requires recruitment...Chromosome-specific repetitive loci can be efficiently visualized in living cells using a single gRNA that has ...

... Plasmid Interchangeable: usually encodes for VSV-G Interchangeable: usually encodes for VSV-G Safety ...activate expression. The envelope protein Env (usually VSV‐G due to its wide infectivity) is encoded on...in order to produce lentivirus? 293T cells are usually used to produce lentivirus. What dictates lentiviral...

...functionally equivalent called the Pribnow box which usually consists of the six nucleotides, TATAAT. Proximal... span a wide range of DNA sequences. It is not unusual to have several regulatory elements such as enhancers...

...type of horizontal gene transfer (conjugation), usually providing a benefit to the host, such as antibiotic... can be used to select or sort the cells by visualization or FACS). Primer Binding Site A short single-stranded...