Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 581 results for: SUB

Showing: 201 - 220 of 581 results

  2. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...license.    Pro tip! Subtypes of immune cells are often defined by a subset of the markers used to ...you’re interested in studying immunology or subpopulations of cells, you’ll soon find yourself encountering...

  3. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... Cas13a binds and cleaves RNA rather than DNA substrates. In terms of structure, Cas13a shares no homology...Cas13a as a diagnostic tool, as discussed below. Subscribe to CRISPR Blog Posts Applications of Cas13a   ...hundreds of observed shRNA off-targets. They subsequently used catalytically inactive dLwaCas13a as a ...

  4. How to Write a Scientific Review Article

    Type
    Blog Post
    ...textbook. Get the journal’s submission rules for review articles Whether submitting a review by invitation ...for their feedback on the scope and the content. Submit your article! Overall, writing a review can be ...

  5. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ... data is to be made at the time of manuscript submission. The rationale includes increasing the ability...individual video. To PLOS’s credit, the organization subsequently clarified what the policy intent was and provided...be argued various authors have simply chosen to submit elsewhere). The majority of the scientific community...

  6. Tips for Getting a Faculty Position

    Type
    Blog Post
    ... to Get a Faculty Position. This edition has substantial expansions on the chalk talks and Zoom interviews.... video interviews. For point of reference, I submitted my applications all by snail mail and only had... me and I'll try to answer your question and subsequently update the ebook. One of my graduate students...

  7. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...around the lens. It allowed me to focus on the subject after I’ve left the room. Microphone: Lastly, I...shirt. But knowing I would have multiple interview subjects, I positioned a boom mic just out of frame allowing...camera from a distance if I needed to be close on a subject, or I could manually control the camera by hand...

  9. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...counter-selection system relies on the sacB gene product of B. subtilis (Hmelo et al., 2015). The sacB gene product is...single crossover and integrated into the genome. Subsequent selection on sucrose will identify double crossovers...after about 48 hours, the resulting colonies turn subtly blue or magenta. This is particularly valuable ...

  10. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...a glycoprotein that consists of four identical subunits that can each bind one molecule of biotin. Biotin...surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting...to attach proteins or polypeptides to a solid substrate (Holden and Cremer, 2003). References 1. Chapman-Smith...

  11. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...microtubule-destabilizing drugs are impossible to target to specific subcellular regions, laser ablation can damage other cellular...After 30 minutes of blue light illumination, substantial PA accumulates in the plasma membranes, indicated...reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular...

  12. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...wet transfer system the sandwich is completely submerged in a tank filled with a transfer buffer formulated...that emits light upon reacting with a specific substrate. The emitted light, or chemiluminescence, is detected...immunoprecipitation and are a common readout for subcellular fractionations, a procedure that isolates proteins...

  13. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...published and unpublished experiments on similar subjects in a single, searchable space. Finally there are...2018). In this ideal situation, a researcher would submit an experimental design to a journal prior to performing...platform for pre-registration wherein researchers can submit designs and transparently reveal them upon completing...

  14. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...these fragments can be cloned into E. coli and subsequent proteins screened for cell-binding abilities ...proteins directly onto the nitrocellulose for subsequent probing for cell-binding activity.  First, cut...room temperature. After this, it is helpful to subject your membrane to several freeze/thaw cycles to ...

  15. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...antigen to myeloma cells. The hybridomas are then subcloned by limiting dilution, which produces an immortalized...based on molecular weight by SDS-PAGE, and are subsequently transferred to a membrane. A primary antibody...of antibodies is used to both identify the cell subsets of interest from among the mixture and to measure...

  16. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...Fluorescence refers to the emission of light by a substance (fluorophore) that has absorbed light or electromagnetic...enzyme variant that covalently reacts with a small substrate ligand motif attached to a fluorophore or other...on a protein, enabling the labeling of protein substrates/peptides and antibodies. Example applications...

  17. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...is it? What parts of it move when you feed it substrate or add an essential cofactor? How many binding...especially since the potential rewards could be substantial: Plasmid tRNA Synthetase Expression Host ...Ermolenko, Dmitri N., et al. "Observation of intersubunit movement of the ribosome in solution using FRET...

  18. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...eluting the DNA from the substrate. In a column-based approach, the substrate is a silica matrix fixed...the plasmid DNA from your cleared lysate to a substrate, washing away left-over debris that carried over...

  19. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...contain various promoters and reporters, and subsequently inserted into a Cas9-containing destination ...inserted. The BsaI site remains intact, allowing subsequent “bricks” to be added one by one. This approach...time needed for a single round of editing, and a subsequent curing protocol that requires only 2-3 hours ...

  20. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...phosphatase treatment, substituting cytidine with 5-methylcytidine, and/or substituting uridine with pseudouridine...selection is removed. Yu et al found that ⅔ of subclones generated from EBNA1 iPSCs still had episome DNA...
Showing: 201 - 220 of 581 results