We narrowed to 731 results for: Sis;

... more functional benefits to the host such as resistance to antibiotics, degradative functions, and/or...advantageous for survival, such as an antibiotic resistance gene. In contrast, plasmids utilized in the ...recruiting replication machinery proteins Antibiotic Resistance Gene Allows for selection of plasmid-containing... obtained. Selectable Marker The antibiotic resistance gene allows for selection in bacteria. However...

...Primer design. Plasmid mapping. DNA sequence analysis. We all have our favorite tools for tackling these...plasmid Editor): A free, donation-based plasmid analysis tool including editing, annotating, creating maps...pET21a-BirA.   DNA / protein database search and analysis tools Addgene's analyze sequence tool: Annotates...Assembly  OligoAnalyzer Tool from IDT: For hairpin analysis, Tm and primer dimer estimation, and other primer...

...multiple high-profile publications. My classmates consisted of graduate students, post-grads, and early career...topic by the moderators. The assignments, which consisted of reading that class’s section of a manuscript...Four ways to improve your next manuscript 1. Be consistent with your figures The figures of a manuscript...and they should also be easily understood and consistent in design and layout. 2. Be concise in your writing...

...kinematics in physics, to developing a lab about mitosis and meiosis in biology. Lots of it is researching the... to students, like how to pour a gel [for] electrophoresis or doing a dissection video. I did that in ...themselves. “You open up the box and there’s a gel electrophoresis tray in there. Or the dissection specimens....

...earlier than most companies. Since the start of this crisis, we’ve been very responsive to the mood of the ...unknown amounts of time, we were facing a financial crisis. As a nonprofit organization, Addgene charges enough...figure out a way to maintain our services during the crisis and maintain our workforce for when we need to ...plasmids and other reagents every day, and this crisis has highlighted the importance of access to quality...

...learn about trends in synthetic biology and DNA synthesis. They published their results in a paper in Nature...biology, in particular, the ability to do DNA synthesis, is evolving so fast that it is a really nice ... in GC content between many organisms and DNA synthesis companies often work within a narrow range for...Philipp Pfingstag, and Neil C. Thompson. "Gene synthesis allows biologists to source genes from farther...

...The researchers identified MerMAIDS from their analysis of different publicly available metagenomic data... study demonstrates the power of combining the analysis of publicly available metagenomic datasets with...fused dCas9 to a truncated CIB1 protein from Arabidopsis (CIBN), which they call the LADL Anchor. Using... iterations is the addition of CRY2, another Arabidoposis protein that heterodimerizes with CIBN upon ...

...dosing, we advise that users keep titering method consistent across samples within a project. Targeting Your... on vector dose. The color diversity in Vector Assisted Spectral Tracing (VAST) experiments using PHP....best practices’ for model organisms.    Q11: How consistent is expression is when injecting CAP-B10 or CAP-B22...linked to epigenetic changes, such as multiple sclerosis?  A: Basic science and preclinical researchers...

... lost a specific gene survive (ex: antibiotic resistance marker). In a previous blog post, we covered ...plasmid backbone, as it often relies on antibiotic resistance. But what about identifying clones that contain...Lynne Postovit’s lab. It contains an ampicillin resistance marker in the backbone. A selection on agar containing...visualize this process, or visit our restriction analysis blog post. Colony PCR Colony PCR can detect...

...mechanism by which bacteria have gained antibiotic resistance and virulence. Scientists have studied how HGT... replication-incompetent phage particles with assistance from a ‘helper’ phage prior to transduction. ...can infer genetic advantages such as antibiotic resistance. Bacterial conjugation as explained by...Bacterial Conjugation. In An Introduction to Genetic Analysis. 7th edition (2000). Meibom, Karin L., et al. ...

...of common plasmid features such as antibiotic resistance genes, selectable markers, promoters, origins...plasmid sequence For more immediate, high-level analysis, you can click directly on the “Analyze Sequence...to additional tooltip information and sequence analysis tabs including: Maps This is the first tab...For example, if you hover over the ampicillin resistance gene, you’ll find the full name of the gene, ...

...expression profiling, systematic tumorigenesis assays, drug epistasis assays, and morphological profiling...huge challenges preventing us from testing this hypothesis: There was no large scale, broadly available... corresponding to cancer variants derived from analysis of lung adenocarcinoma genomes (Lung Cancer Mutant...

...newly synthesized product from a site-directed mutagenesis experiment? The answer to both questions is the...basepairs or small indels that occur during DNA synthesis, and protomoting or repressing protein expression...cleave DNA. DpnI is often used for site directed mutagenesis. During this process, incorporation of a desired...Troubleshoot Your Sequencing Results Use REPLACR Mutagenesis to Mutate Your Plasmids Resources on Addgene.org...

...will catch you up on the core replication and resistance features of yeast vectors and explain how they... and diploid state which is useful for genetic analysis, as well as an efficient mechanism of homologous... due to high rates of spontaneously occurring resistant mutants and the insensitivity of yeast strains...auxotrophic markers. The MX series of antibiotic resistance cassettes is most commonly employed, with the...

...Protein turnover is the combined effect of protein synthesis and degradation. These processes control the abundance...possible to visualize the localization of protein synthesis. Alternatively, fluorescent protein tags can be...the system. Another method for studying protein synthesis in situ is the knock-in of fluorescent tags into...Nathans, Daniel. "Puromycin inhibition of protein synthesis: incorporation of puromycin into peptide chains...

...that is resistant to the toxic effects of CcdB. Another commercially available, CcdB-resistant strain ... investigator’s gene of interest.   CcdB resistant E. coli strains complete the system But ccdB alone...contains the F plasmid (F’ strains) will also be resistant to CcdB, as the native ccdA will be present. Most...

... of replication, protein tags, and antibiotic resistance markers (just to name a few). In this edition...site-specific recombination events. The system consists of two components derived from the P1 bacteriophage...sites are 34-base-pair long recognition sequences consisting of two 13-bp long palindromic repeats separated...targeting, targeted clones are selected for using a resistance marker; however, it is often desirable to remove...

...gotten myself.” Perkins noted that there is a more emphasis today about non-academia and industry jobs. Since...her first job at Addgene as an Order Processing Assistant. After a few months, she left school when she ...mean you’ve failed. “I got a job as a research assistant in a lab at the university that I went to. Making.... She was working as a research and technical assistant, when her life changed––she had a daughter. Needing...

... 1000000097) The Winslow lab barcoding library consists of 96 retroviral plasmids (based on the Murine...high-throughput sequencing to perform multiplexed analysis of cells pre-treated with a compound of interest...Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time to produce virus? Get the ...mammalian cells. The MuLE system kit from the Frew lab consists of 91 building block Entry vectors and 5 lentiviral...

...makes them ideal candidates for applications in oncolysis and vaccination!) In the 65 years since its discovery...virion is a non-enveloped particle of ~100 nm, consisting of a capsid surrounding an inner core which contains...adenovirus: the virion is ~ 100nm in size, and consists of an icosahedral capsid made of 3 types of proteins... for creating adenoviral vector constructs. It consists of 2 plasmids: a shuttle vector (in which the ...