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We narrowed to 616 results for: TIL

Showing: 201 - 220 of 616 results

  2. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...but not thermophilic (high heat loving) bacteria. Until recently the only available Cas9 proteins were isolated... GeoCas9 RNPs to be incubated at high temps but still maintain activity following heat treatment. The ...

  4. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...and transformation. While restriction cloning is still routinely performed by many labs, a variety of other... of cloning has its advantages, most scientists still encounter several struggles with these techniques...

  5. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...response to tissue damage, have excessive bone growth until eventually the patient loses mobility. Attempts ...novel solutions to treat rare diseases like FOP until a cure is available. Addgene and AJ's Avengers This...

  7. Viral Vectors 101: Transductions

    Type
    Blog Post
    ... Molecular basis of transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into...are non-integrating (adenovirus and AAV) but can still express episomally long-term (AAV). If you perform...

  8. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...isotype conversion is routinely used to increase the utility of recombinant antibody tools, the resulting chimera...post has answered all of your questions about the utility of isotype conversions and chimeric antibodies!...

  9. What the HEK?

    Type
    Blog Post
    ...the fetal tissue which HEKs were isolated from is still a mystery, but it’s currently assumed that the cells... on another cell or organism. This pipeline is utilized to transduce cell lines for basic science research...

  11. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...injection. How long post-injection did you wait until performing the experiment? What detection methods...data. Please note that the data hub is currently still in beta version, so we’re always looking for ways...

  13. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...are incredibly useful, chemical fluorophores can still be used in a wide variety of experiments. Read more...and to find a video of them in action. AAV: A Versatile Viral Tool for Gene Expression in Mammals Adeno-associated...

  14. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...researchers lamenting the viral production process while still ordering viral vectors. Virus production can be ...Beginners Lentiviral Vector Uses and Overview AAV: A Versatile Tool for Gene Expression in Mammals Additional...

  16. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...scientific or science communication. A Tweet is still considered scientific communication if the language...non-jargon in the context of disease testing, it can still be considered jargon in most other uses. Many laypeople...

  18. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ... well-designed fragment overlap sections, while still creating scarless plasmid inserts.  Want to Learn...digestion, and used directly in DNA assembly will still yield scarless assemblies. Overview of NEBuilder...

  20. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...many more viral particles to your solution while still using a volume that’s compatible with your experimental... Control: Going the Extra Mile with NGS AAV:  Versatile Viral Tool for Gene Expression in Mammals Additional...
Showing: 201 - 220 of 616 results