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  1. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...but not thermophilic (high heat loving) bacteria. Until recently the only available Cas9 proteins were isolated... GeoCas9 RNPs to be incubated at high temps but still maintain activity following heat treatment. The ...
  2. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...Here are just 4 of the CRISPR-Cas9 FAQs. If you still have more questions after reading through these,...expression, though it is possible for the plasmid to still express without the G. Because the G is only one...
  3. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...are incredibly useful, chemical fluorophores can still be used in a wide variety of experiments. Read more...and to find a video of them in action. AAV: A Versatile Viral Tool for Gene Expression in Mammals Adeno-associated...
  4. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...researchers lamenting the viral production process while still ordering viral vectors. Virus production can be ...Beginners Lentiviral Vector Uses and Overview AAV: A Versatile Tool for Gene Expression in Mammals Additional...
  5. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...injection. How long post-injection did you wait until performing the experiment? What detection methods...data. Please note that the data hub is currently still in beta version, so we’re always looking for ways...
  6. Antibodies 101: Chimeric Antibodies

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    Blog Post
    ...isotype conversion is routinely used to increase the utility of recombinant antibody tools, the resulting chimera...post has answered all of your questions about the utility of isotype conversions and chimeric antibodies!...
  7. What the HEK?

    Type
    Blog Post
    ...the fetal tissue which HEKs were isolated from is still a mystery, but it’s currently assumed that the cells... on another cell or organism. This pipeline is utilized to transduce cell lines for basic science research...
  8. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...scientific or science communication. A Tweet is still considered scientific communication if the language...non-jargon in the context of disease testing, it can still be considered jargon in most other uses. Many laypeople...
  9. Viral Vectors 101: Transductions

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    Blog Post
    ... Molecular basis of transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into...are non-integrating (adenovirus and AAV) but can still express episomally long-term (AAV). If you perform...
  10. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...technique, I try to look for a resource to help me. I’m still waiting for a mass spectrometry app. I digress. ...one convenient resource. I can safely say that I still have the single and triple abbreviations of the ...
  11. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...many more viral particles to your solution while still using a volume that’s compatible with your experimental... Control: Going the Extra Mile with NGS AAV:  Versatile Viral Tool for Gene Expression in Mammals Additional...
  12. Cultivating Community Science at BosLab

    Type
    Blog Post
    ... prior to getting started. For example, Timothy Stiles joined BosLab last year with a computer science...developed community projects. Recently, Timothy Stiles and Mrina Nikrad have started the Open Yeast Engineering...
Showing: 201 - 220 of 607 results