We narrowed to 617 results for: TIL;
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TypeBlog Post...Until recently, there were no completely genetic tools that would allow researchers to label just a fraction... Applications for SPARC SPARC technology is versatile and can be applied across cell types and organisms...
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FastCloning: A Newer, Simpler Cloning Technique
TypeBlog Post...and transformation. While restriction cloning is still routinely performed by many labs, a variety of other... of cloning has its advantages, most scientists still encounter several struggles with these techniques... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post...response to tissue damage, have excessive bone growth until eventually the patient loses mobility. Attempts ...novel solutions to treat rare diseases like FOP until a cure is available. Addgene and AJ's Avengers This... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog Post...analytical thinking, time management, information distillation, etc) are transferable to many different fields...thinking about preparing yourself for a career while still in graduate school, you’ll be well suited to follow... -
Viral Vectors 101: Transductions
TypeBlog Post... Molecular basis of transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into...are non-integrating (adenovirus and AAV) but can still express episomally long-term (AAV). If you perform... -
Antibodies 101: Chimeric Antibodies
TypeBlog Post...isotype conversion is routinely used to increase the utility of recombinant antibody tools, the resulting chimera...post has answered all of your questions about the utility of isotype conversions and chimeric antibodies!... -
What the HEK?
TypeBlog Post...the fetal tissue which HEKs were isolated from is still a mystery, but it’s currently assumed that the cells... on another cell or organism. This pipeline is utilized to transduce cell lines for basic science research... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post... to visualize individual/non-overlapping cells. Until now, tools for doing this were labor-intensive and...intermediate (12%), and sparse (4%) levels. SPARC is a versatile system that can be used in many cell types and... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog Post...injection. How long post-injection did you wait until performing the experiment? What detection methods...data. Please note that the data hub is currently still in beta version, so we’re always looking for ways... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog Post...cabbage and kale to name a few. Despite Arabidopsis’ utility to science, and its well-known edible relatives...include growing plants require pots, trays, soil, fertilizer, seeds and a growth space with fluorescent lights... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog Post...Addgene plays in accelerating research. Yet this was still not enough to ensure we could keep up with demand...organizations to request support for our operations until our normal work resumed. Addgene distributes and... -
10 Great Guest Posts We're Thankful For
TypeBlog Post...are incredibly useful, chemical fluorophores can still be used in a wide variety of experiments. Read more...and to find a video of them in action. AAV: A Versatile Viral Tool for Gene Expression in Mammals Adeno-associated... -
Using Addgene's New Viral Service for Your Research
TypeBlog Post...researchers lamenting the viral production process while still ordering viral vectors. Virus production can be ...Beginners Lentiviral Vector Uses and Overview AAV: A Versatile Tool for Gene Expression in Mammals Additional... -
Intro to Our Summer SciComm Series!
TypeBlog Post...scientific or science communication. A Tweet is still considered scientific communication if the language...non-jargon in the context of disease testing, it can still be considered jargon in most other uses. Many laypeople... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog Post... described more than 60 years ago, but there is still no treatment available. Aguilar is a basic cell ...described in 1971,” McSweeney said. “But, it is still sometimes wrongly diagnosed as a seizure disorder... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog Post...libraries are some of Addgene’s most exciting and versatile offerings! We recently re-amplified our distribution...library, this is less of a concern, but Addgene still recommends plating the transformation mixture on... -
Pushing the Limits of DNA Assembly
TypeBlog Post... well-designed fragment overlap sections, while still creating scarless plasmid inserts. Want to Learn...digestion, and used directly in DNA assembly will still yield scarless assemblies. Overview of NEBuilder... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post... convince him to use Twitter @jonchowphd but he still reads “#” as a “pound symbol”. References 1. A. ... A. Elliott, A. H. Brand, The GAL4 System : A Versatile Toolkit for Gene Expression in Drosophila, 3, ... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog Post...do we get where we want to be? The part that is still missing is a clear alignment of incentives. Right...is clearly room to do more. If we could really instill a culture of rewarding good scientific citizenship... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog Post... an improved apoptosis reporter, ZipGFP, which utilized the self-assembling split GFP (GFP1-10 and GFP11...Drosophila, an observation that has been difficult until now (Zhang et al., 2019). Conclusion FlipGFP is ...