...the steps and technical considerations are directly applicable to the rat [6]. Technical differences include..., regardless of the type of behavior, certain technical considerations still apply. Testing stimulation...is wrong or 2) there is some problem with the technical implementation of your optogenetics setup (viral...difficult as it seems. Like every other advanced technique in neuroscience it has a bit of a steep learning...neuroscience sub-discipline that has published using the technique and ask if they would be willing to teach you....performed in this species. However, transgenic rat technology is improving and may one day reach the sophistication... et al. "Recombinase-driver rat lines: tools, techniques, and optogenetic application to dopamine-mediated...

... that brings to light the cellular world. The technique allows you to ask questions like: “Where does ...within a cell,” “Does this disease change the architecture of my cells,” or “How does this mutation impact...whole organism. But, as with any antibody-based technique, validation and proper experimental design are... a future blog post to learn more about these techniques!  Direct vs. indirect immunofluorescence There...approach than direct IF, so we will focus on this technique here. One of the reasons that indirect IF is more...Figure 2. For a deeper dive into the steps and techniques, see Im, et al. 2019.  Fixation Good fixation...has shed some light on immunofluorescence. The technique can provide you not only answers to your most ...

... system will you use to detect your fluorescent protein? The type of detection method depends on what ...Experiment  Detection Method Notes Quantification of a process Plate-based assays (plate reader) Detect the... are biological tools that monitor a process or detect a given molecule. The sensor component is usually...conformational change in response to the molecule it detects. This change then generates a reporter signal. ...have summarized some of the common fluorescent detection methods and the types of experiments they are ...

... that works for your application, specifically detects your protein, is species compatible, and doesn’...review the most common tags, the antibodies used to detect them, and how they can fix your antibody woes.   ...antibodies for the purpose of purification or detection of the tagged protein. These peptides consist ...an antibody. Tags can enable the separation of detection of a mutant protein vs. endogenous WT levels of... blog, though, we are focusing on tagging for detection purposes (stay tuned for our affinity purification... be more!) to a protein’s termini to increase detection. The tag can also be removed by treating the protein...fundamentals to commercial systems. Appl Microbiol Biotechnol. (2003) 60:523-533 DOI: 10.1007/s00253-002-1158...

... of this approach is SnapGene’s algorithm for detecting common features. This algorithm enables one of...identified common variants, and then crafted a detection algorithm that tolerates occasional mismatches...to require at least 96% sequence identity when detecting a reference feature. For a coding sequence feature...feature that may be used to make fusion genes, detection needs to occur even if one or two codons are missing...versions of common coding sequence features, so our detection system was enhanced to allow searches for a perfect...sequenced strains through our mismatch tolerant detection algorithm and thereby speed up the annotation ... see an opportunity to harness common feature detection in a new way, please contact us at snapgene.com...

...wasn’t that easy. In the 1980s, gene targeting technology was invented to introduce specific changes into...into the mouse genome. With these early technologies, a researcher would first introduce a mutation into...transgenic offspring. Although powerful, this technology is cumbersome to use, not that efficient, often...first exposure to CRISPR when he used this new technology to engineer mouse models (Wang et al., 2013, ...others around the world. Yes, finally, we have a technology, CRISPR, that is simple in concept, straightforward...electroporated into the oviduct of pregnant females, a technique called Genome editing via Oviductal Nucleic Acids... germline transmission of the transgene, this technique is still in need of improvement. Also when working...

...replicates such as multiple mice in each group and C, technical replicates or repeated measurements will increase... in biological experiments. Replicates can be technical or biological and are included to improve the ...therefore the statistical power of an experiment. Technical replicates are repeated measurements of the same...nonspecifically through the Fc, allowing you to detect this undesirable interaction. When non-specific...with the preadsorbed antibody. If a signal is detectable, it is likely due to binding interactions outside...the signal measured. If a nonspecific signal is detected, the level of noise will determine whether it ...flow cytometry. A viability control allows you to detect dead or dying cells so that you can base your analysis...

...molecular biology labs. Recent and rapid advances in technologies that permit large-scale creation and synthesis...overcome crucial challenges in NGS by developing technologies that can help unlock the potential of modern...develop and apply our plexWell™ Library Preparation Technology for NGS-based sequencing and confirmation of ...plexWell™ in more detail, and how we are using this technique in our partnership with Addgene to sequence large...large numbers of plasmids. plexWell™ technology overview For any NGS experiment, the DNA sample being...samples. As mentioned above, one of the primary technical benefits of plexWell™ is that normalized libraries... and CEO of seqWell, Inc. He is a sequencing technologist and computational biologist with a passion for...

...! In most academic and nonprofit institutes, the Tech Transfer department is responsible for managing ...deposit a plasmid with Addgene, your organization’s tech transfer office is sent a deposit agreement. The...approach, which we’ve updated and refined as new technology becomes available. Our one-time deposit agreement...agreement means we only have to work with one tech transfer office when materials are requested, instead ...organization upon purchase. We recognize exceptionally fast tech transfer offices with the Golden Stopwatch award...questions about our MTA process, you can check out our Tech Transfer Webpage, help center articles, and our ...information. Happy transferring!    Many thanks to our Tech Transfer team members Maura Coughlin, Darcy Morse...

... and sequence displays are now powered by GSL Biotech's SnapGene Server Software. With the backing of ..., tags, and certain ORFs. Their sophisticated detection algorithm easily and precisely identifies plasmid... Many of these features, such as Cas9, weren’t detected by our in-house software. In fact, SnapGene’s ...includes nearly 3x as many features as could be detected by our in-house software and also eliminates many...within the plasmid. The list of enzymes that can be detected is 5x greater than our previous mapping software...The table found here lists the common features detected in the plasmid sequence. The table includes feature...contains a table listing commonly used primers detected in a given nucleotide sequence. The table includes...

...contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans Health Administration...roles and my current work as a AAAS Science and Technology Policy Fellow (STPF) at the Veterans Health Administration... come from a wide variety of backgrounds with technical expertise that ranges from astronomy to bioethics...these resources compiled by the AAAS Science and Technology Policy Fellowship Program to learn more about...could understand how to make new treatments and technologies accessible to patients. And what better place...guest blogger Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans Health Administration... Guerra, PhD, is currently a AAAS Science and Technology Policy Fellow at the Veterans Health Administration...

... time working on interesting biotechnology code.There are valid technical reasons to choose a non-Python...particular plasmid and now it’s time for our lab technicians to retrieve the sample and get it ready for shipping...simply follows a linear progression directing our techs through the process. The problem? As we said above...to do when something fails. While we’re sure our techs could figure it out if they needed to, they’d have... when a tricky procedure failed and one of your techs started over with a new tube. In the first example...science_name`. You'll need to decide given the technical details of the problem you're solving, but consistency...

...higher order oligomers can be analyzed by several techniques. Gel filtration and SDS-PAGE under non-denaturing... Whether higher molecular weight complexes are detected will depend on the homodimer affinity and the ...qualitative view of dimerization tendency. Another technique that separates molecules based on size is ultracentrifugation...based on fluorescence polarization) can be used to detect homodimerization. However, all the aforementioned...these assays. However, these strategies will not detect weak dimerization tendency, as is present in EGFP... a cell-based assay that does a better job of detecting homodimerization tendency. This assay employs ...substantial (e.g, the poor performance of mRuby2 is detected by Constantini et al (2015) and by Bindels et ...

...Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...specific loci has historically been difficult, as techniques like fluorescent in situ hybridization (FISH)... locus...unlike CRISPR. Early CRISPR labeling techniques allowed researchers to visualize nearly any single...and movement of this locus in live cells! This technique was developed in the lab of Addgene depositor ... the method is specific and sensitive enough to detect gene copy number based on the number of fluorescent...to mapping pairs of intrachromosomal loci. The technique successfully resolved loci with physical map distances...and engineered sgRNAs using CRISPRainbow.” Nat Biotechnol. 34(5) (2016): 528-30. PubMed PMID: 27088723....

...generate a targeted break. These advances in gRNA technology have enhanced targeting efficacy as well as the... as they will be recognized as foreign RNA. To protect the RNA from degradation, several simple methods...added to the 2’ hydroxyl group of the ribose to protect it from nucleolytic attack. Another stabilizing...just one modification alone (Hendel, et al., Nat Biotechnol; Rozners, JACS). These modifications can be done...switches for gRNAs A major drawback of CRISPR-Cas9 technology is that most expression systems are constantly...system can initiate targeted editing events. Both technologies utilize chemical modifications to the RNA to...CRISPR-Cas genome editing in human primary cells. Nat Biotechnol 33, 985–989 (2015). https://doi.org/10.1038/nbt...

... mice had no detectable editing. Additionally, off-target spleen editing was not detected and Nanoblade...efficiency and off-target effects compare to existing technologies, and their use for HDR editing and mouse-model...compared head-to-head with existing CRISPR delivery technologies, Nanoblades had higher rates for editing efficiency... targeting the Fto gene, while editing was not detected in cells electroporated with RNPs using the same...cytoplasm or pronucleus of the zygote which is both technically challenging and can negatively impact zygote ... from Tyr Nanoblade treated zygotes, five had detectable Tyr editing at the phenotypic and genotypic levels...

...attempted to use the technique for epitope tagging but only one of them could detect knock-in of tags at...NgAgo survey Since it was first published in Nat Biotech in May 2016 (1), NgAgo has received much attention...indel formation (gene disruption) with this new technology. To dig more into what researchers felt about...responded to the survey. When asked if they could detect indels (an indication of gene disruption ability...genome editing with NgAgo, this does not mean the technology is dead in the water. On the bright side, successful...Natronobacterium gregoryi Argonaute." Nature biotechnology (2016). PubMed PMID: 27136078. 2. Burgess, Shawn...

... post was contributed by Kevin Esvelt, a Wyss Technology Development Fellow at the Wyss Institute and ...widespread trust in scientists to develop new technologies with care and humility. Nothing would damage...could not be trusted to handle such a powerful technology responsibly. An accidental release would be particularly...Finally, gene drives are an inherently collective technology: because they necessarily alter the shared environment...Thank you to our guest blogger! An inventor of technologies that harness evolution, Kevin Esvelt studies...ensuring that gene drives and other collective technologies are developed safely and transparently.  For...101: a blog series on the basics of CRISPR-Cas technology CRISPR Resources from Addgene   ...

...partners with other techniques! Minimizing false negatives: Interactions leading to detectable reporter signals...expression (Figure 2c). The reporter gene then allows detection of those cells where binding between the proteins...as using five independent protein interaction detection methods (6). In order to bind DNA and activate... Fields, S., Song, O. A novel genetic system to detect protein-protein interactions. Nature. 1989; 340...Y.l. Split luciferase as an optical probe for detecting protein-protein interactions in mammalian cells... D., and Frommer, W.B. K+ channel interactions detected by a genetic system optimized for systematic studies...

...endeavor including but not limited to biotechnology, chemistry, architecture, engineering, mathematics, physical...developments.  Further, this post may contain technical inaccuracies or typographical errors.  No action...non-bench research career such as law, medical/technical writing, clinical, regulatory, product development... sector are transferrable to industry such as technology transfer, communications, medical writing, compliance...your expert skills your letter should include technical information followed by a plain language explanation...Casseus Law. Driven by her own love of science and technology, Rachel is proud to assist companies and foreign...professionals.  We work with organizations in the biotech, medical device, and diagnostics, healthcare IT...