...made it easier to create knockout alleles in a variety of species, including the standard laboratory mouse...see deletions of the region between the gRNAs. A variety of other undesired outcomes, including one loxP...Easi-CRISPR for simple knock-ins and floxed models varies from 8.5-100% of pups born, with typical efficiencies...30-60%. Quadros et al. attribute much of this variation to differences in guide RNA efficiency. For example...correctly edited founder. Figure 6: Diagram of ovary and oviduct anatomy with the equipment used for ...female mice using a small incision that exposes the ovaries and oviducts. After solution injection into the...

...served as the Chief Human Resource Officer for Harvard Medical School. The addition of Cicalese to the...member. When I was working in Human Resources at Harvard Medical School, Joanna was my colleague. She mentioned... committee because I had recently retired from Harvard, and it let me keep my toe in the water of HR work...understand the science but my prior HR experience at Harvard Medical School means I understand the needs of ...served as the Chief Human Resources Officer for Harvard Medical School where she brought leadership teams...

...Matthew Canver of Boston Children’s Hospital and Harvard Medical School. Addgene is proud to present a video...paired double-strand break (DSB) approach at a variety of loci. We were pleasantly surprised by the efficiency...investigator at Boston Children’s Hospital and Harvard Medical School. His lab studies the molecular genetics...bauerlab.org.     Matthew Canver is an MD/PhD student at Harvard Medical School in the laboratories of Stuart Orkin...erythroid enhancer of BCL11A subject to genetic variation determines fetal hemoglobin level. Science 2013...

...relies on kinesin. Different rates of speed for various forms of retrograde and anterograde transport suggest...visual, and auditory neuronal circuitry in vivo. A variant of RABV has been developed to only label a neuronal...proteins VP1, VP2, and VP3. To date several hundred variants, or serotypes, have been found naturally or have...Janelia Research Campus also developed a new AAV variant, rAAV2-retro, that migrates retrogradely at higher...toxicity, higher specificity and reduced technical variability compared to chemical tracers. rAAV2-retro also...viral preparations of AAV2-retro vectors with a variety of transgene/promoter combinations. Check out our...D.V., and Karpova, A.Y. (2016). A designer AAV variant permits efficient retrograde access to projection...

...Global Head Muscle Diseases at Novartis, opened with a discussion of the Novartis industry postdoctoral program...postdocs, Kristin Krukenberg and Jessica Polka from Harvard Medical School and a long list of supporters from...Lahav, Associate Professor of Systems Biology at Harvard Medical School, titled her presentation “How to...Marc W. Kirschner, Shirley Tilghmanc and Harold Varmus     ...

...the genomes of many different fish species in a variety of non-functional forms and is believed to be millions...system was synthesized by fusing and modifying various extinct transposases. Improvements have been made... extinct transposon sequences and then testing various combinations of changes. The basic functional version...Zsuzsanna Izsvak’s lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts...inducible expression vectors were developed into 16 variants with different selection markers (blasticidin,...

...developed an adaptive immune system that utilized various cas genes not only to store a record of invading... prokaryotic species suggest the presence of a variety of undiscovered viral anti-CRISPR genes. These ...specificity, orthogonality, and multiplexibility in various species and describe an assortment of applications...specific, programmable platform well-suited for various innovative biological and translative technologies... enabling access to multiple plasmids used for various platform applications. For more information on ...

...chemical fluorophores can still be used in a wide variety of experiments. Read more to find chemical fluorophores...a postdoctoral researcher in the Silver Lab at Harvard University, describes R-bodies, “force generating...commonly used to deliver a genes of interest to a variety of cell types in-vivo. These are widely used by...respectively. Didem Goz Aytruk from Connie Cepko’s Lab at Harvard Medical School describes how AAVs function and ...proteins, dCas9, and a modified gRNA to direct a variety of customizable functions to your DNA sequence ...

...research to explore a variety of phenomena, and so too can it be used to teach a variety of science concepts...Brassicaceae family, Arabidopsis is related to a variety of food crops such as broccoli, cabbage and kale...concepts. From inheritance and variation, to growth and development, and response to environment - ABRC has... conduct the experiments outlined in ABRC’s kits vary by module. As a general guideline, experiments that...

... Although we only walk one path at a time, the variety of paths in life is mind-boggling. Unlike a mountain... editor In my role as an editor at Science, my various interests come together as useful and productive...or policy makers. The term editor covers a wide variety of careers and jobs. At Science, which is published...Fellows Program). Science journalism programs at various universities range from week-long workshops to ...engagement through science communication with a variety of audiences. She focuses at Science on developmental...

...and heavy chains made up of variable regions and constant regions. Variable regions are unique to each ...Figure 1: In the isotype conversion process the variable regions of a parental antibody (blue, rat) are...resulting chimeric antibody would have the original variable regions from the parental rat antibody but the... tools. In the case of isotype conversion, the variable regions of a specific antibody are cloned into...

...behaviors often fall short with the generation of various flux imbalances. The conventional approach has ...engineering have been limited to the overexpression of various metabolites in Escherichia coli or Saccharomyces...modular synthetic RNA scaffolds that can recruit various transcriptional regulators (Zalatan et al. 2015...RNA scaffolds. These RNA scaffolds can recruit various transcriptional regulators to alter gene expression...metabolic flux. The dCas9 protein and orthogonal variants can be used as regulatory ON/OFF master switches...

...cancer cells but are directed not to through these various signals. Over-production of vascular endothelial...made to use whole cell therapeutics consisting of various types of immune cells. Dendritic cells (DCs) are...Check out this review for an in-depth look at a variety of up and coming therapies. Future directions ...Cell heterogeneity Tumors are composed of a wide variety of cell types. This cell heterogeneity is an important...of the genotypic and phenotypic changes in the various types of cancer will fuel the development of immunotherapeutics..."Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts...

... build single- and multi-gene constructs for a variety of applications. MoClo tools can be used in bacterial...Keep in mind that this technology is useful for a variety of purposes beyond what we discuss. If you can ... multi-TU constructs (Moore et al., 2016). The variety of constitutive and inducible promoters, linkers...Beyond sgRNAs, MoClo can also be used to test a variety of Cas enzymes with different PAM requirements ... MK system, many of the parts can be used in a variety of organisms, saving time and money for labs with...the Modular Cloning System.” PLOS ONE, edited by Alvaro Galli, vol. 13, no. 5, Public Library of Science...

...blogger Jon Chow, an immunology PhD student at Harvard University. In this second post in our quick guide...background. In other cases, YFG or one of its mutant variants will need to be overexpressed or knocked down....Stock Center has fly lines expressing Gal4 under a variety of promoters resulting in tissue specific expression... You can quickly mate your UAS-YFG line with a variety of Gal4 lines and study gene expression in many...Chow. Jon Chow is finishing his immunology PhD at Harvard University. He has had nearly a decade of experience...

...sequences (colored rectangles) are cloned into various plasmids using oligonucleotides. These plasmids...depending on which method you use, the procedure will vary slightly.   Two Golden Gate options that are... with the Gersbach lab plasmids, multiple Cas9 variants are available: wt humanized Cas9, D10A nickase...clone your own entry vectors containing other Cas9 variants to use with the MuLE system. Multiplexing from...into one of 12 pYLsGRNA plasmids, which contain various promoters and reporters, and subsequently inserted...Topic Page Get Advice on Choosing The Proper Cas9 Variant for Your Experiment Learn About Multiplexing with...

...expressing various gRNAs. It’s better to compare the phenotypic effects of expressing various gRNAs in ...choice after transduction with this virus. While various Cas9 mutants have been engineered to perform unique...challenging. This cytotoxicity also causes growth rate variability when using lentiCas9-blast to generate Cas9-expressing... vitro and in vivo (Krashes et al., 2011). The various serotypes available (AAV2, AAV5, AAV8) enable different...

...right for me? Here’s a list of some important variables to consider when choosing an inducible promoter...cell types or organisms, promoters are similarly variable. Bacterial promoters only work in prokaryotic ...species from which they were derived. Similarly, the various eukaryotic cell types (mammalian, yeast, plants...target gene? The strength of inducible promoters can vary a lot. If you’re looking for high inducibility, ...time The time needed to induce a given promoter varies. Promoters that require only the addition of an...

... active variant, the phage is not infectious. The editing speed dictates which phage variants can propagate...complete ABE protein. If the phage delivers an active variant of the base editor, a functional gene III product...stringency PACE, resulted in the evolution of TadA variants with much higher base editing activity compared... TadA-7.10 enzyme. After testing numerous TadA variants, they identified TadA-8e, which edits 590-fold...

...specific destinations, haul a variety of cargo, and even handle a variety of terrains. They are, in fact..., large collections of biological independent variables, are becoming increasingly popular tools with ...your library cargo to isolate the effects of each variable being manipulated. Thus, the adjustable nature...efforts. And Addgene is here to help! We offer a variety of viral vectors, viral services and even a Viral...