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  1. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

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    ... we can make Addgene better and make scientists’ lives easier. Why transition to Addgene? So why did ... that PhD. Resources at Addgene Read Other Careers Posts Visit the Addgene Careers Page Learn about ...Harvard University and entered a new role here at Addgene as an Outreach Scientist. I used to spend my days... less time wrangling bacteria and instead help Addgene fulfill its mission of helping scientists share...did I join Addgene? Was I jaded by my time in academia? Did I run screaming from the lab like the cartoon...didn’t, it would have been unwise of me to join Addgene. This is particularly true in my position as an... here! Thus far being an Outreach Scientist at Addgene has been awesome. The process of writing, editing...
  2. Download Science-Themed Zoom Backgrounds from Addgene

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    ...default backgrounds all year and want a change? Addgene has you covered. Today we’re releasing 11 bold ...reading this blog, chances are you are a fan of Addgene or our very cute mascots!  Blugene has been helping...might want to use one of our virus backgrounds. Addgene’s viral service provides ready-to-use AAV and lentiviral...the joke? If not, you might want to check out Addgene’s Fluorescent Proteins blog topic to learn more ...background.     You can find all of the plasmids that Addgene offers by visiting our collections page. Here, ... green DNA background.     Since we ❤️ DNA at Addgene, we're presenting you with another DNA background...transformations with the plasmids you receive from Addgene? Our bacteria background is probably a good fit...
  3. Hot Plasmids - August 2020

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    ...pMAK461(NBαRabitb-IgG-Fc, Addgene #140699) pMAK462 (NBαMouse/Rat-IgG1-Fc, Addgene #140700) pMAK463 (NBαMouse-IgK...NBαMouse-IgK, Addgene #140701) pMAK464 (NBαEGFP, Addgene #140702) The pMAK461-64 plasmids were expressed...the plasmids to set up your screening systems at Addgene! Schematic of the evolution platform to generate...fluorescent mammalian cell structure markers plasmids at Addgene! Chertkova, AO, et al., bioRxiv. 2020. https://...and dCas9-VPR targeting plasmids can be found at Addgene. Check out these plasmids for piggyBac gRNA delivery...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources...human lung epithelial cells. Find the plasmids at Addgene The MoClo CRISPR/Cas Toolkit for plants includes...
  4. Learning Technical Skills at a Lab Central Workshop

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    ...Resources at Addgene.org Find pooled libraries at Addgene Find basic lab protocols at Addgene ...information from the workshop. Future workshops for Addgene Addgene strives to be a great resource for the research...Technician at Addgene who is interested in medicine and music.       Additional Resources on the Addgene Blog ...As a tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, ... use what we learn to improve processes in the Addgene lab. In this post, I’ll tell you a little bit about...excited to go there because both LabCentral and Addgene aim to make scientific advancement easier. NEBNext...libraries as opposed to replicating them like we do at Addgene), going to the workshop was a great opportunity...
  5. Why Do I Need an MTA Anyway?

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    ... Here’s how Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!)... on collaboration (it’s the heart and soul of Addgene’s mission), but legal complexities can arise when...institutions require them for material transfers. When Addgene entered the plasmid sharing space, we heard over...confidence. Benefits of the UBMTA: Peace of mind Addgene has adopted the terms of the Uniform Biological... review by the Provider Limited liability Addgene makes it easy Even with all those benefits, we ... handle all the paperwork. Record Keeping: Addgene will let you know exactly how many times your materials...Streamlined Processing: When you place an order, Addgene generates the MTA and emails it straight to you...
  6. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ...information to Addgene Material Transfer Agreement (MTA) approval  Send plasmids to Addgene  Quality control... field, and contact Addgene. We may require that you send your samples to Addgene pre-transformed in this... to appear in your Addgene account under My Plasmids, we need to know your Addgene account username. Shipping...  Additional Resources on the Addgene Blog Find Articles Citing Addgene Plasmids Searchable and Sortable...the spreadsheet to deposit@addgene.org with the following information: Addgene account username. In order...Suggest A Plasmid You'd Like to Find at Addgene Resources on Addgene.org Start Your Deposit Deposit FAQ Which...deposit spreadsheet. The data entry process On the Addgene home page, if you scroll over “Deposit” in the ...
  7. Stepping into a Virtual Internship: My Experience at Addgene

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    ...their roles at Addgene. I had a great time speaking with Quintin Marcelino who is Addgene’s Science Video...change. Having been at Addgene for a month, I can say with certainty that Addgene is a company that cares... continuing Addgene’s Meet an Addgenie Series on Instagram. I interviewed fellow Addgenies about their...like to work at Addgene. This is exemplified in my work interviewing fellow Addgenies. I spoke with amazing...Sociology and Dance at Skidmore College. I was also Addgene’s Marketing Intern this summer! With the help of...made me intrigued by the marketing internship at Addgene. Although my interests are very clear, I had no...COVID was getting, I was hoping to walk into the Addgene office my first day and meet everyone but this ...
  8. Celebrating One Million Plasmids Shared!

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    ...fit in a freezer). Our Addgene celebration was so much fun! Here's the Addgene Leadership team in all ...excitement amongst our Addgenies on social media or around the office. @Addgene congrats for 1 million... a million plasmids would fill? Two weeks ago, Addgene edged passed our one million plasmids shared milestone...might have you wondering how many plasmids leave Addgene headquarters every day. That would be an average...our shipping room above), but as Joanne Kamens, Addgene’s executive director, said during our celebratory...it takes to make one plasmid and how much time Addgene has saved researchers. Assuming 5 hours of hands-on... — BryanRoth (@zenbrainest) August 21, 2018 As Addgene grows, we have been able to adapt to the needs ...
  9. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

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    ...became a member of the Addgene advisory board where he will help shape Addgene's future. Listen to this...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...In this episode of the Addgene podcast, we sit down with Alex Chavez, a Clinical Fellow in Pathology ...Institute. 33:09 - end : How Alex hopes to help Addgene advance - plants for trimmed down protocols, distribution...us.  Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board with a particular interest...Additional Resources on the Addgene Blog Learn More About Alex's Work with Cas9 Activators Michael Koeris...CRISPR Lentivirus Learn about Other Members of the Addgene Leadership Team ...
  10. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

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    ...in China Additional Resources on Addgene.org Learn About Addgene's Mission Check Out Our International...As we mentioned a few months ago, Addgene has been working with Seeding Labs to provide plasmid grants...and Duedu and how they plan to use plasmids from Addgene to advance their research. Louis Bengyella Dr. ...would like to use the plasmids he acquires through Addgene to produce molecular biological tools like Taq ...also use polymerases produced using plasmids from Addgene to test for the presence of pathogens in environmental...first round of awards has successfully concluded, Addgene and Seeding Labs will be opening applications for... applicants will propose for plasmids from the Addgene community and hope we can do even more to enable...
  11. How to Deposit Your Plasmids with Addgene

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    ... DNA library with Addgene?" or contact us at deposit@addgene.org or +1 (617)-ADDGENE (233-4363) with additional...Reference the Addgene ID in the "Backbone Manufacturer" box for backbones requested through Addgene. For example...for You: Find Articles Citing Addgene Plasmids Resources on the Addgene Website Learn the Benefits of...deposit@addgene.org Special cases Please contact us at deposit@addgene.org or +1 (617)-ADDGENE (233-4363...strain. Please contact us at deposit@addgene.org or +1 (617)-ADDGENE (233-4363) if you’d like to distribute...At Addgene, it is our mission to make it easy for you to share plasmids. To achieve this goal, we will...online, you will need to create an account with Addgene before starting the process. After you have logged...
  12. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

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    ... a position on the Addgene Board of Directors. We're excited to watch both Addgene and Sample 6 grow with...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Good Manager from Addgene Executive Director Joanne Kamens Resources on the Addgene Website Check Out...installment in our podcast series, we chat with new Addgene Board Member, Michael Koeris. Dr. Koeris did his...developing? 38:00 - End : Dr. Koeris' role on the Addgene Advisory Board and how he hopes to help young biotech...Bacteriophage-based Diagnostics Additional Resources on the Addgene Blog Check the Other Posts in Our Podcast Series...of our Board of Directors Become a Part of the Addgene Community ...
  13. Why and how to start studying chromatin (with Addgene's help)

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    ...sharing your plasmids through Addgene, don’t hesitate to start the process! Addgene depositors who study chromatin...associated PI pages on the Addgene website. In-exhaustive List of Addgene Depositors Who Study Chromatin...pii: 1722491115. Addgene blog posts related to chromatin This is not the first Addgene blog post about ...phenomenon, there’s a reasonable good chance that Addgene can help. Though it would be difficult to consolidate...in the chromatin field who have deposited with Addgene, but you’ll find many great tools by clicking on...Chromatin-related genes with plasmids available from Addgene The links below pull up lists of plasmids containing...you can subscribe to these pages to learn when Addgene receives new plasmids containing these genes. As...
  14. Addgene is Expanding Our Viral Vector Service!

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    ...Subscribe to the Addgene newsletter for updates on all things Addgene; or subscribe to the Addgene blog for updates...request an AAV composed of an AAV plasmid in the Addgene repository and their choice of five different serotypes...comes to ordering high-quality materials from the Addgene repository. Read on to learn more.  What will be... be available?  Over 3000 AAV plasmids in the Addgene repository will be available for Packaged on Request...request. Request details can be accessed within your Addgene account. Preps will be available for request in...Once submitted, the request will be reviewed by Addgene for legal approvals and technical compatibility...Cart” button will appear with the request in your Addgene account.  Orders do not need to be placed immediately...
  15. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

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    ...that, when you cite a plasmid from Addgene using its unique Addgene ID number, your publication will be...Feature Browse More Inside Addgene Posts Additional Resources on Addgene.org Browse Our Special Collections...plasmid technologies contributed by our depositors, Addgene strives to make it easier for you to find the plasmids...sophisticated search, and curating plasmid collections. Addgene's "How to cite this plasmid" section Anytime you...repository, we request that you cite it using both its Addgene ID number as well as the reference for its original... process, we explain how to cite plasmids from Addgene right on our plasmid pages and provide a citation...Why do this? Not only does citing plasmids from Addgene help others find them later if they need to use...
  16. QC Sequencing Technologies at Addgene

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    ...Resources on the Addgene Blog A Look at Addgene’s QC Process Bioinformatics at Addgene The Power Behind...technologies that Addgene uses in the QC process, when these technologies are used, and how Addgene keeps up with...Validation: seqWell Addgene’s Tips for Plasmid Quality Control Additional Resources on addgene.org Molecular ...provided a general overview of the QC process at Addgene. All plasmids go through this same initial QC process...Illumina MiSeq, but that’s not the only technology Addgene uses to ensure accuracy of deposited plasmids. ...bioinformatic analysis are occurring every year. At Addgene, we want to maintain the highest quality and accuracy... technologies. Sequencing technologies used at Addgene Sanger sequencing (“First-generation sequencing...
  17. Celebrate National Book Month with Addgene

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    ...Communications Manager Addgene's book club Indeed, reading is ingrained into Addgene’s culture. We have a ...still going on today! Addgene Book Club (ABC) founder, Eric Perkins, is Addgene’s Director of Science Product... of Addgene’s culture. Have you read any of these books? Whether you have or not, let the Addgene community...Additional resources on the Addgene blog Learn about company culture at Addgene Read about other lunch activities...From Addgene Book Club to our on-site book swap/library, reading is part of the culture here at Addgene...and explains the origins of ABC: I founded the Addgene Book Club (aka ABC) in 2011 after having numerous... activities at Addgene ...
  18. Plasmids 101: NGS Quality Control for Pooled Libraries

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    ...CRISPR Libraries Resources on Addgene.org Browse Pooled Libraries at Addgene ...In addition to single plasmids, Addgene also distributes pooled plasmid libraries containing hundreds...million plasmids. These libraries are some of Addgene’s most exciting and versatile offerings! We recently...plasmids are amplified and none are lost. Each Addgene library has its own depositor transformation and...components of the library are represented. Typically, Addgene uses electrocompetent Stbl4 cells when performing...transformation efficiency. For a typical library, Addgene usually does four to eight electroporation reactions...a gRNA library, this is less of a concern, but Addgene still recommends plating the transformation mixture...
  19. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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    ...two-color pair. We'll be sending it to Addgene in a matter of days. Addgene: Can you tell us a little more about..., or other conditions. Addgene: You've contributed various plasmids to Addgene over the years, many of...available. Visit Ed Boyden lab's Addgene page and click the link in the Addgene Alerts box. References: ...activity of two populations of neurons at once. Addgene spoke to Boyden about this latest optogenetics ...missing. Optogenetic tools for neuroengineering Addgene: For those who may not be experts, can you give...Understanding the brain through optogenetics research Addgene: What are some of the big problems that optogenetic...these tools, so we can focus on the next round. Addgene: What tools are still missing from the optogenetics...
  20. New Viral Vectors - Summer 2024

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    ... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share...to our viral vectors repository since March.  Addgene is now offering an AAV2 CAG-driven tTA expresser...tool called PdCO, deposited by the Yizhar lab. Addgene is now offering AAV1 and AAV5 preparations of several...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...the full viral services collection or check out Addgene's Data Hub to see viral vector reports from other... the link below to keep up with the latest in Addgene's viral vector services and resources!   This blog...
Showing: 201 - 220 of 1091 results