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...working at Addgene?” Most Addgenies reply with an enthusiastic, “Our company culture! Addgene is the best... Benjie Chen co-founded Addgene in 2004. They worked tirelessly to get Addgene from a concept to an up... culture here at Addgene has remained paramount. Candidates who interview at Addgene frequently ask the...ideas and company. The Addgene summer picnic This past weekend was the annual Addgene Summer Picnic - a time... it was another successful event! Addgene company culture At Addgene we organically developed the philosophy... happened naturally when Addgene was a family affair back in 2004. As Addgene has grown, it has taken ...Our story today starts with a bit of Addgene history... As you may be aware, Melina Fan, her brother ...

...number of CRISPR libraries Addgene has distributed (1C), from 2012 to 2023. Addgene’s collection of CRISPR ...As we celebrate twenty years at Addgene, we are sharing stories of our repository’s positive impact on...community. They did this by depositing them in Addgene’s repository. Other researchers, eager to try this... tools of their own to share. Over the years, Addgene’s repository has grown to include many CRISPR tools...This growth is supported by new labs joining the Addgene CRISPR community, with approximately 100 new CRISPR...CRISPR depositors each year.    Figure 1: Addgene CRISPR distribution and deposits. (A) Cumulative... (B) Cumulative CRISPR plasmids deposited with Addgene by year. (C) Cumulative number of laboratories ...

...All plasmids deposited at Addgene enhance scientific sharing worldwide but a select few are particularly...we know biological resource centers, including Addgene, positively affect scientific research. As Maria...Maria Castro of University of Michigan says, “Addgene has transformed the way in which we share plasmids...tool is so influential that it is requested from Addgene many times as the technology disseminates throughout...researchers who have at least one plasmid deposited at Addgene that has been distributed more than 100 times. ... on sharing because, as UNC’s Bryan Roth says, Addgene has “literally enabled sharing with 100's of investigators...everything from every paper is routinely deposited in Addgene!” As you can imagine, answering plasmid requests...

...seller. However, Addgene is a bit different from other vendors. As a nonprofit, Addgene does not own the...here at Addgene. If you’ve called our customer service line or sent an email to help@addgene.org, you’ve...plasmids online is a little trickier -- but at Addgene, we try to make the process as smooth and simple... bigger question: How does a customer place an Addgene order using a PO number? Why do I have to order...distribute it on behalf of depositing institutions. Addgene cannot fulfill orders based on PO documents that... with the recipient organization can create an Addgene account and place an order: a purchasing agent,... order using a PO number? Placing an order on Addgene’s website is quite simple, even if you’re using ...

... on addgene.org Addgene's Antibody Collection Addgene's AAV Data Hub More resources on the Addgene blog... request for your experiment!  Addgene’s Antibody Data Hub Addgene’s Antibody Data Hub can help with ...open-access Data Hub, researchers interested in the Addgene antibody collection can look at user-deposited ...experiment.   Figure 1: The front page of the Addgene Data Hub.   Antibody data reports can be searched...each data submission we receive is checked by Addgene's curation team for accuracy and completeness. The... citation. If you’re considering requesting an Addgene antibody, the Data Hub can help you decide if an...your experiment. If you’ve requested and used an Addgene antibody, we encourage you to submit data showing...

...we will not delete @addgene on X. This will additionally ensure that the @addgene handle is always associated...media, you can find us on Bluesky: @addgene.bsky.social; or @addgene on Instagram, TikTok, Facebook, and...an official Addgene account. You can reach out to us directly via email at help@addgene.org, and we invite...Addgene is announcing we have chosen to stop all activity on X, formerly Twitter. If you are interested...mainstay of conversation in the scientific community. Addgene has been considering a gradual move off X as a ...to paid content regardless of user preference. Addgene is a values-driven nonprofit. Recent remarks and...posting, monitoring, and resharing content on X.  Addgene deeply values our community and recognizes the ...

...with Amy C., Addgene’s intern coordinator shortly after hearing her talk about Addgene’s internship opportunity...environment changes them. Addgene gave me the chance to become a real life scientist. Addgene gave me the chance...knowledge more than ever before. Before joining Addgene, I had spent most of my junior year stressed out...applying for summer programs. I spent one day at Addgene last summer, through the La Vida Scholars summer...opportunity taking place next summer.  I applied to Addgene and began the interview process with Amy, who let...March that I successfully secured an internship at Addgene! I was eager to accept my position, but I was waiting...whether or not you work in the lab. My first week at Addgene, I had to get used to the workspaces, as well as...

...sequenced by Addgene’s QC team. Email help@addgene.org to get started! Addgene’s QC process Addgene prioritizes...Quality Control Now at Addgene Bioinformatics at Addgene Resources on Addgene.org Educational Resources... Figure 2. Addgene’s basic QC process. Created with BioRender.com. Once Addgene receives deposited...receiving the replacement plasmid at Addgene. QC is a team effort Addgene’s QC team is full of dedicated scientists... Resources Additional resources on the Addgene blog Addgene's Tips for Plasmid Quality Control Plasmids...bacterial growth. Created with BioRender.com.   At Addgene, we put a lot of effort into our quality control...sequence, as they were deposited before 2017 when Addgene started sequencing all incoming plasmids. If you...

...behind-the-scenes peaks of Addgene’s people, culture, and workplace. Rumor has it that a few Addgenies* may even be ...means summer is right around the corner. Here at Addgene, we’re always inspired by the new seasons, so we've...We’re very excited to be launching the official Addgene TikTok at the end of this month! We’ll be sharing... be posting in the next few weeks.)  Finally, Addgenies love sunshine and warm weather - check out our...

...times easier. We’ve certainly felt this way at Addgene so we’ve been putting some work into finding robots... many more robots than you ever knew existed! Addgene recently started automating a few protocols to ...concerning genetically modified organisms. Since Addgene is very proud to help scientists share plasmids... hour. Automating vial fiIling The majority of Addgene plasmids are shipped as agar stabs in glass vials... easily programmed for a variety of lab needs. Addgene uses it to fill as many vials as it can in the ...avoid sore fingers. There are other process that Addgene would love to move forward and automate. For instance...comments section.   Additional Resources on the Addgene Blog Apps for Lab Life Developing Lab Management...

...improving the tools you use to share and access them. Addgene’s product, development, and content teams have made...resources, tools, collections in our catalog, and the Addgene Help Center. A big part of this redesign was pulling... place, so you can quickly navigate around the Addgene website without scrolling up and down the page....’ll find Blugene, as well as information about Addgene, our mission, links to our deposit, request, and...skim through. Users with disabilities will find Addgene’s website easier to navigate, thanks to design choices... made some changes to the look and feel of addgene.org. Some you’ll notice right away, while others may...— check out all the new features and designs addgene.org! And when you’re finished, feel free to take ...

...Production AAV distributed by Addgene has been produced either in-house by Addgene scientists or through collaboration...Browse AAV preps available from Addgene. After receiving your virus from Addgene, read our detailed recipient...lentiviral preps available from Addgene. After receiving your virus from Addgene, read our detailed recipient...references Resources at the Addgene blog Find and share AAV Data with Addgene's Data Hub Digital Droplet ...Addgene has a growing catalogue of viral preps available to request. In this post, we’ll walk you through...are titered and subjected to quality control by Addgene before being distributed to customers. Details ... -80 °C. Titer Titering is either performed by Addgene or by the University of Pennsylvania Vector Core...

...social media. #AddgeneCRISPRweek on Twitter From May 13 (today!) to May 17, the Addgene Twitter feed will...CRISPR. Hope you will join us this week at #AddgeneCRISPRweek. We updated the CRISPR guide! If you’re not...

...Halloween is only 2 days away and here at Addgene we couldn’t be more excited! As a small, tight-knit...email system.  Our 2013 winners dressed up as Addgene's favorite lunch... a BonMe noodle meal, crammed...interested in finding out who wins this year’s Addgene costume contest, like us on Facebook and checkout...  addgene...celebration this Friday will combine all of these. Addgenies have been organizing Halloween festivities for...

...grants and Addgene’s mission This is the first NIH grant that Addgene has received. Addgene prides itself...allow Addgene to further our mission of scientific sharing,” says Dr. Daniela Bourges, Addgene’s interim...antibodies Resources on Addgene.org Find recombinant antibody plasmids at Addgene ...to improve upon these critical research tools. Addgene is excited to announce that we have recently received...Neurotechnologies® (BRAIN) Initiative, will allow Addgene to create a much needed open-access recombinant...recombinant antibodies in this blog post. Hello, NABOR! Addgene will bring the same open-access benefits to antibodies...single-chain variable fragment (scFvs) and nanobodies. Addgene will produce a subset of this collection as ready-to-use-proteins...

...learn what Addgene can do to help. More resources on the Addgene blog Twenty Years of Addgene Sharing: ...researchers around the globe. Addgene’s educational resources As Addgene built out our scientific support...other videos on Addgene’s YouTube channel.   Figure 4: Global viewership of the Addgene YouTube channel...about virus, I learned from Addgene,” or “I learned to clone from the Addgene website.” We delight in seeing...When Addgene opened in 2004, we focused on creating a repository that made it easy to share plasmids,...accessible, due to a wide range of logistical barriers. Addgene worked to identify, reduce, and remove these logistical...accessible weren’t always the same thing, shaped both Addgene’s founding and our growth into the company we are...

...in the Addgene repository each year from 2016–2023. B) AAV preps distributed per year by Addgene from 2016...available at Addgene from 2016–2023 by type of tool. B) AAV preps distributed by Addgene per year from...As part of Addgene’s 20th anniversary celebration, we’ve been finding and sharing stories unique to our... our repository and resources. As editor of Addgene’s eBooks — including the recently released Viral Vectors...the model organism, injection site, and titer. Addgene’s Viral Vector Service Besides being complex to ...quality of the prep. This was a large factor in Addgene’s decision to launch our viral vector service in...able to order high quality viral vectors from the Addgene repository can save researchers significant time...

...ICYMI: Addgene’s Plasmids 101 eBook Protocols and Plasmid References @Addgene  ...Plasmid" list. Email us at help@addgene.org with the Plasmid Name, Addgene Plasmid ID #, and the PubMed ...Exciting news! Addgene recently rolled out a new feature on our plasmid pages - links to articles citing...systems it has been validated in.  If a plasmid's Addgene ID # has been referenced in other publications,... see where they've been cited. Benefits to the Addgene community of scientists Benefits to depositing ...Properly citing plasmids you've requested from Addgene may get your paper listed in the "Articles Citing...you know of an article that used a plasmid from Addgene, we want to hear about it so we can link to it ...

...Community Addgene: In 2012 your lab first deposited plasmids with Addgene. In 2014, you joined Addgene's Advisory...lab. In June 2014, Dr. Fujii joined Addgene's Advisory Board.  Addgene: Your lab has worked extensively ... enChIP through Addgene. Retroviral Expression System for enChIP using CRISPR Addgene: Your lab recently...the plasmids of these systems to Addgene in the near future. Addgene: Has your lab been working on any...first knew Addgene around 2010 and found the scheme very nice at first sight in that Addgene takes care...ourselves without Addgene. So, when I received an invitation to join the Advisory Board of Addgene, I accepted...Hodaka Fujii lab's Addgene page and click the "Subscribe to Alerts" link in the Addgene Alerts box. References...

...features of Addgene's website Resources on Addgene.org Try out the new search on Addgene's homepage ...been working on behind the scenes here at Addgene.  Addgene's new homepage While users are increasingly...emphasize the values that make Addgene unique: community, sharing, and fun. The Addgene repository is built on...  Additional resources on the Addgene blog Learn more about Addgene's new search tool Read more about...-over and our new homepage? If you've visited Addgene’s homepage recently, you probably noticed some big...access the content they need. All within seconds.  Addgene’s old homepage had been relatively the same for ...shares reagents and knowledge with one another, and Addgene make this process easy and fun every day.  With...