Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 18 results for: addgene

Showing: 1 - 18 of 18 results

  1. Lentiviral Vector Guide

    Type
    Guide
    ...vectors can be found in Addgene’s stable cell line protocol . Browse Addgene's collection of in-stock ... Viral Vectors 101 eBook Addgene's Biosafety Resources Resources on Addgene's Blog Quick Guide to All ...Resources on addgene.org Lentiviral Plasmids Collection In-Stock Lentiviral Preps Addgene's Viral Prep ...the end! Browse lentiviral plasmids available at Addgene. Lentivirus Versus Gamma-Retrovirus Lentiviruses...versa. For more about gamma-retroviruses, see Addgene's gamma-retroviral vector guide . Lentiviral Vectors...information on cloning and working with plasmids, visit Addgene’s Molecular Biology Reference . Production After...a transgene. For more detailed protocols, see Addgene’s viral vector protocols . Figure 5: Overview of...

  2. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...can find more information in Addgene’s stable cell line protocol . Addgene’s protocol is for lentiviral ...Vector topics Addgene protocols Generating Stable Cell Lines Using Lentiviral Vectors Addgene's Viral Protocols...Resources on addgene.org Gamma-retroviral Plasmids Collection Viral Vectors 101 eBook Addgene's Biosafety...the end! Browse retroviral plasmids available at Addgene. Gamma-Retrovirus Versus Lentivirus Lentiviruses... vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide . Gamma-Retroviral Vectors...information on cloning and working with plasmids, visit Addgene’s Molecular Biology Reference . Production Using...viral titer. For more detailed protocols, see Addgene’s viral vector protocols . Production Using Packaging...

  3. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...by visiting Addgene's Optogenetics Guide . Browse optogenetics plasmids available at Addgene. CRISPR/Cas9...Vectors 101 Youtube Videos Addgene's Biosafety Resources Resources on Addgene's Blog Parts of the AAV Transfer...In-Stock AAV Preps Resources on addgene.org AAV Plasmids Available at Addgene Viral Vectors 101 eBook Viral...glossary at the end! Browse AAV plasmids available at Addgene. Adeno-Associated Virus versus Adenovirus Adenoviruses...information on cloning and working with plasmids, visit Addgene’s Molecular Biology Reference . Production The three...vivo models. For more detailed protocols, see Addgene’s Viral Vector Protocols . Figure 3: Overview of...systemic capsids for more information. Browse Addgene’s in-stock viral preps . AAV retrograde (AAVrg) ...

  4. Molecular Biology Reference

    Type
    Guide
    ...videos for specific techniques and tips! Addgene Protocols Addgene Video Library...Addgene's molecular biology reference guide, including information on molecular genetics, plasmids, sequencing... plasmid. For commonly used primers check out Addgene's sequencing primer list. Working with Plasmids ...empty plasmid backbone for your experiment, see Addgene's empty backbone page for more information. Expression...genome. For more information on CRISPR check out Addgene’s CRISPR guide . Reporter Plasmids - Used for studying...infect your target cells at a high efficiency. Addgene's expanding viral service offers select ready-made...strains below and recommend checking out these two Addgene blog posts relating to common E. coli lab strains...

  5. Adenovirus Guide

    Type
    Guide
    ...Viral Vectors 101 eBook Addgene's Biosafety Resources Resources on Addgene's Blog An Introduction to Adenovirus... References Resources on addgene.org Adenoviral Plasmids Available at Addgene AdenoBuilder Toolkit Viral...the end! Browse adenoviral plasmids available at Addgene. Adenovirus versus Adeno-Associated Virus Adenoviruses...information on cloning and working with plasmids, visit Addgene’s Molecular Biology Reference . AdEasy™ AdEasy™,...construct is then transformed into AdEasier-1 cells (Addgene #16399) , which are BJ5183 E. coli cells containing...viruses. The adenoviral backbone plasmid pAdEasy-1 (Addgene #16400) is suitable for most purposes. For especially...especially long transgenes, the use of pAdEasy-2 (Addgene #16401) can increase the capacity of the rAdV vector...

  6. Educational Resources

    Type
    Guide
    ...Overview of Addgene's educational resources, including eBooks, science guides, videos, and protocols ... content , including protocols, tips on using Addgene’s materials, and career advice. Playlists include...Protocols Careers How-to Blog Read about plasmids in Addgene’s repository , and related molecular biology techniques...

  7. Addgene's eBook Collection

    Type
    Guide
    ...start? Need a quick reference? Addgene's eBooks are here to help!... Addgene's eBook Collection Not sure where...where to start? Need a quick reference? Addgene's eBooks are here to help! 0 of 0 Selected Download Select...

  8. Plan Your Experiment

    Type
    Guide
    ...AAV size limitation . Browse Addgene's in-stock AAV preps or try Addgene's Packaged on Request service....appropriate left and right homology arms. See Addgene's HDR blog post for more details. In some cases,...the reference sequence you used for gRNA design. Addgene offers many plasmids containing validated gRNAs...plasmid in question (see CRISPR protocols from Addgene depositors ). Browse empty gRNA expression vectors...you may need to look into other options. Browse Addgene's collection of CRISPR plasmids . Delivery Using...conducting genome-wide screens using CRISPR . Browse Addgene's collection of in-stock lentiviral preps . AAV ...

  9. Guide to Using Pooled Libraries

    Type
    Guide
    ...the Library Once you receive your library from Addgene, check the library information to see if it should...crucial for conducting pooled library screens. Addgene does not offer next-generation sequencing services...our Pooled Library Amplification Protocol for Addgene's generalized library amplification protocol. Read...

  10. Sequencing Primers

    Type
    Guide
    ...A list of sequencing primers that Addgene uses for sequence verification of deposited plasmids....Reference Sequencing Primers Sequencing Primers Addgene has used a number of primers for sanger sequence...convenience. For reference information, please consult Addgene's Molecular Biology Reference Page . All listed ...sequences listed are provided for your reference. Addgene does not distribute primers. For sequencing plasmids... sure what primer you need? Email us at help@addgene.org...

  11. Chemogenetics Guide

    Type
    Guide
    ...Addgene's guide to using Chemogenetics plasmids in your lab for interrogation of neuronal activity.... find chemogenetics plasmids available through Addgene, check out our Chemogenetics Plasmid Collection...alternative DREADD ligands, please see the text. Browse Addgene's collection of DREADD plasmids ! PSAMs Unlike DREADDs...Neuronal activation Magnus et al., 2011 Browse Addgene's collection of PSAM plasmids ! LMOs Luminopsins...Neuronal inhibition Ikefuama et al., 2025 Browse Addgene's collection of LMO plasmids ! Planning Your Chemogenetics...

  12. Promoters

    Type
    Guide
    ...Addgene's molecular biology reference material covering promoters, transcription and the transcription...small RNAs, such as shRNAs and gRNAs. Resources Addgene's blog , including our popular Plasmids 101 series...

  13. Optogenetics Guide

    Type
    Guide
    ...Addgene's guide to using Optogenetics plasmids in your lab, including explanations of microbial opsin...To find optogenetics plasmids available through Addgene, check out our Optogenetics Plasmid Collection ...previous tools through directed mutagenesis - use Addgene's Optical Switch Plasmid Table to search for plasmids...

  14. Cloning

    Type
    Guide
    ...Addgene's reference guide on molecular cloning techniques, including information on seven commonly used...to assemble before you get started. Check out Addgene's website for easy to use MoClo and Golden Gate ...

  15. Science Guides

    Type
    Guide
    ... Science Guides Addgene has created the following guides to help you start...

  16. Modular Cloning Guide

    Type
    Guide
    ...cloning (MoClo) and find MoClo kits deposited with Addgene, including the MoClo Toolkit and the MoClo Plant...et al., 2011. MoClo Plasmid Kits Available from Addgene Browse or search the table below to find MoClo ...

  17. CRISPR Guide

    Type
    Guide
    ...window) . Addgene Website & Blog References Website CRISPR plasmids and resources at Addgene CRISPR 101...cell lines Viral service at Addgene Viral plasmids and resources at Addgene Blog CRISPR topic page How ...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...You Use a CRISPR Library? CRISPR libraries from Addgene are available in two formats: as liquid DNA, or...located. The majority of the CRISPR plasmids in Addgene’s collection are from S. pyogenes unless otherwise...

  18. Antibody Guide

    Type
    Guide
    ...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production...applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...intensive. Ready to put your new knowledge to use? Addgene now makes ready-to-use recombinant antibodies made...
Showing: 1 - 18 of 18 results