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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 643 results for: ato

Showing: 201 - 220 of 643 results

  1. Cancer Research Plasmids and Resources

    Type
    Collection
    ...and Libraries for Cancer Research Broad Target Accelerator Plasmids: Collection of vectors from the Boehm...mutant alleles described in the initial Target Accelerator publications, focused on somatic variants found...

  2. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...reporting and genetically encoded fluorescent voltage indicators (GEVI) are ideal tools to track bioelectric states...channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization in continuous...Use of genetically encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (...

  3. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...(Shaner et al, 2008). Obligate dimers (such as dTomato) that promote interactions between the FPs interfere...CytERM-mKOkappa, CytERM-mTurquoise2, and CytERM-dTomato. When using and interpreting the results of the...proteins. Fusions of CytERM with mTurquoise2 and dTomato, which are established monomeric and dimerizing...

  4. Developing Lab Management Software for Biology

    Type
    Blog Post
    ... Laboratory management software is not a requisite for a functioning lab, but it is for a scaleable lab...about what steps are most likely to fail. Our laboratory manager would definitely be interested to see...this case, the physical considerations of the laboratory make object composition a smarter choice than...

  5. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...Library, deposited by Carrie Bhang, a research investigator in the In Vivo Pharmacology group at Novartis...ClonTracer Since starting her new role as an investigator in the In Vivo Pharmacology group at Novartis...Addgene community! Carrie Bhang is a research investigator in In Vivo Pharmacology at Novartis Oncology...

  6. Chemogenetics Plasmids

    Type
    Collection
    ...Drugs (DREADDs) and Pharmacologically Selective Actuator Modules (PSAMs). DREADDs are the most widely used...optogenetic element, making a bimodal opto-chemogenetic actuator. When the luciferase enzyme oxidizes its substrate...

  7. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Find CRISPR plasmids to study the regulatory networks involved in retinal development....mouse retina. To aide in their studies of the regulatory networks involved in retinal development the ...

  9. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...They play important roles in many cellular and regulatory processes, but much of their functionality is...effects can be minimized by disrupting lncRNA regulatory elements that are crucial for transcription and..., on the other hand, recruits transcriptional activators such as VP64, SAM, or SunTag to specific ncRNA...

  10. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...with a terminator. A simple TU could be comprised of just three parts: promoter, gene, and terminator.   ...this category also includes promoters, UTRs, terminators, etc. Once selected, individual units can be ...

  11. 6 Tips for Grant Writing

    Type
    Blog Post
    ...the field. 3. Highlight your network and your collaborators It’s a good idea to move around to different...Networking for Scientists Series 4. Become a science communicator More and more fellowship applications are asking...on your grant application to your peers and collaborators can also help you get some critical insight ...

  12. Recombinases AAV Preps

    Type
    Collection
    ... mCherry 1 Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato 1, 2, 5, 8, 9, rg*, PHPeB Larsen 107787 AAV.TBG.PI.Cre.rBGe...

  13. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...important supporting roles. Enhancers are cis regulatory DNA sequences that, when bound by transcription...CAPTURE (CRISPR Affinity Purification in situ of Regulatory Elements) method. The original CAPTURE method...2020). CRISPR Affinity Purification in situ of Regulatory Elements: The original CAPTURE method The original...

  14. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...air conditioning on) so leave them in a sterile incubator. This also means that any medium or washing buffer...entering the hood and when putting them into the incubator). It also helps to get familiar with the morphology...results. Most cell culture media contain the pH indicator phenol red (pH range 6.0 (yellow) - 8.0 (red))...

  15. PhD Applications After COVID

    Type
    Blog Post
    ...  Is this department/laboratory collaborative with other departments/laboratories? How long is the average...by meeting with professors - a/k/a Principle Investigators (PIs) - whose labs does research that aligns...

  18. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...expressing eGFP under the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate...  The first successful attempt to render fixed anatomical preparations transparent was done by Walter Spalteholtz...protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (3-dimensional imaging...

  19. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato (pH sensors), HyPer ...fluorescent intensity upon pH changes (e.g. pHluorin, pHTomato). Keep this list handy as you plan your next ...

  20. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...communication should be fun and engaging for the communicator as well as the audience, so pick a mode of communication...Otherwise, you’re going to drop your project like a hot potato or burn yourself out trying to slog through unappealing... Low barrier to entry for both audience and communicators. Well-developed content creation platforms.  ...
Showing: 201 - 220 of 643 results