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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 568 results for: bli

Showing: 201 - 220 of 568 results

  1. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...nucleofection The in vivo editing experiments in this publication were performed using a technique called nucleofection...Additonal Corn Lab Members who worked on this publication:         References 1. Richardson, Christopher...

  2. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...available. With new fluorescent proteins being published every month, deciding on the best protein for ...common use (particularly not the most recently published ones), they can be a good starting point. In many...

  3. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...In the blink of an eye, the long days in the lab as a graduate student and postdoc come to an end and...who were “chalk-and-cheese”. While one was an established Professor who was always “off flying his plane...

  6. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...resulting in a fluorescent signal. Since their first publication in 2012, the Nagai laboratory has assembled a...sensitivity and faster imaging compared to previous publications. They also developed calcium, cAMP, and ATP ...

  7. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates Addgene’s founding...plasmid was called pLKO-Tet-On in the original publication but its name was subsequently changed to clarify...

  8. Addgene: The First Twenty Years

    Type
    Blog Post
    ...was frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan...others could request from — Addgene!  Addgene was established as an e-commerce company, which helped it stay...

  9. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following...

  10. Antibodies 101: Conjugation

    Type
    Blog Post
    ...BioRender.com.     Many biologists can lead perfectly blissful, productive lives without thinking about these...10.1016/j.it.2012.02.010 Dennler P, Fischer E, Schibli R (2015) Antibody Conjugates: From Heterogeneous...

  11. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...to find the simplest way to do it (i.e. avoid assembling too many fragments at once, if it is possible...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ...

  12. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ... research environment” with a strong focus on publishing, but still made it so she could “get exposure...summarized in a recent Nature Medicine paper. In the publication, Bhang and coworkers used the ClonTracer library...

  14. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ..., a pre-made MoClo kit, is a prime choice for assembling an array of targeting constructs and delivering... many different MoClo kits have already been established. Keep an eye out for our next blog on choosing...

  15. Hot Plasmids February 2024

    Type
    Blog Post
    ...original, though its maturation is even faster. Published just a few days later, Mohan Balasubramanian and...antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing. In a proof-of-concept study, mouse...

  16. CRISPR in the Clinic

    Type
    Blog Post
    ...substantial relief from both disorders. As of publication, submissions to the UK, EU, and USA federal drug...cardiovascular disease, inflammatory diseases, blindness, and diabetes. Directions and obstacles for future...

  17. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...Cytochemistry (Vol. 46, Issue 8, pp. 977–983). SAGE Publications. https://doi.org/10.1177/002215549804600813 ...Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue 6, p. e38313). Public Library of Science (PLoS). https://doi.org/10.1371...

  20. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...bacteria can also have serious implications for public health. Bacteriocin plasmids: microbial offense...toxic substances like heavy metals or pesticides, enabling their hosts to survive where others can’t. This...
Showing: 201 - 220 of 568 results