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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11 results for: bli

Showing: 1 - 11 of 11 results

  1. Modular Cloning Guide

    Type
    Guide
    ...Cloning (or MoClo) is a system for efficiently assembling multiple DNA parts into functional plasmids. ...genetic circuits, engineering metabolic pathways, assembling gene-editing tools, and more. The MoClo system...Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized parts to create eukaryotic multigene...Plant Expression Daniel Voytas 76 plasmids for assembling plant transformation vectors for overexpression...Plant Expression Benjamin Field 50 plasmids for assembling bimolecular fluorescence complementation experiments...Dueber) for genetic engineering of S. cerevisiae , enabling larger multi-gene constructs, multiplexed CRISPR...and acceptor vectors for generating multi-part assemblies in either integrative or self-replicating plasmid...

  2. CRISPR Guide

    Type
    Guide
    ...Experiment Web References PAM Sequences Glossary Publications CRISPR Overview Bacteria have an interesting...to check the library information and original publication to see if a library is suitable for your experiment...specified by the gRNA plus the genomic PAM sequence Publications (by topic) CRISPR basics Anders, C., Niewoehner.... D., Gross, C. A., & Rosenberg, O. S. (2019). Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi...

  3. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...vectors, they offer the benefit of having well-established protocols for fast and efficient generation of...large-scale, high-titer preps. This is thanks to established retroviral production cell lines that are able...

  4. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...AAVs cannot enter the lytic cycle, and instead establish latent infections by integrating into the host...will no longer be limited to small transgenes, enabling the development of further AAV applications. Read...

  5. Antibody Guide

    Type
    Guide
    ...short peptide linkers. Diabodies can be used for assembling protein nanostructures. Figure 2: An antibody...specific to your antibody and assay may already be published. If it is not, you’ll need to validate the antibody...

  6. Guide to Using Pooled Libraries

    Type
    Guide
    ...relevant to the phenotype being studied. Examples of published selection mechanisms for lentiviral CRISPR libraries...

  7. Cloning

    Type
    Guide
    ... is important to note that LIC has difficulty assembling DNA fragments with repetitive sequences and DNA...

  8. Optogenetics Guide

    Type
    Guide
    ...local concentration near the cell surface, and enabling the ion channel to function. TULIPs Tunable light-inducible...

  9. Plan Your Experiment

    Type
    Guide
    ...with identical genomes. If your end goal is to establish an edited cell line, for example, you will likely...

  10. Adenovirus Guide

    Type
    Guide
    ...remain the most popular choice due to their well-established methods, relative simplicity of production, and...

  11. Molecular Biology Reference

    Type
    Guide
    ...which contains several restriction enzyme sites, enabling easy insertion of DNA by restriction enzymes digestion...
Showing: 1 - 11 of 11 results