We narrowed to 314 results for: bon

...immediate co-transformation with linearized vector backbone. DpnI digestion - 1 μl DpnI is added to the...

...is irreversible — FP fragments become covalently bonded by associating with one another — which means it...

...extraction protocol) disrupts the DNA’s hydrogen bonds with water molecules and coaxes it into sticking...

...Maleimide-labeling occurs when a maleimide group forms a covalent bond with a sulfhydryl group (–SH), such as that found...

...eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins (RNPs) failed to induce off-target editing...

...such as Thioredoxin A (TrxA) that improve disulfide bond formation in order to help keep your protein soluble...

...addition, we have recently developed the oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method ...

...Click here to view Addgene’s collection of empty backbones for constructing fluorescent fusions. Transcription...

...GSG-P2A-GCaMP6f-WPRE-pA Syn GCaMP6f mRuby2 Constitutive 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 51083 AAV-EF1a-...-pA CAG GCaMP6f mRuby2 Cre dependent 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 83899 pAAV-mDlx-GCaMP6f-Fishell...GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Constitutive 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 51082 AAV-EF1a-...-pA CAG GCaMP6s mRuby2 Cre dependent 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 68720 pAAV-hSyn1...-pA Syn GCaMP6s mRuby2 Cre dependent 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 100842 pAAV.CAG.Flex.GCaMP6s.WPRE.SV40...

...-DD and pCS2TALE3-RR are next generation TALEN backbone vectors designed for use with the Voytas Golden...introduced to the N- and C-terminus of the pTAL3 TALEN backbone, which were initially published by Sangamo BioSciences...mammalian cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (...with the Voytas Lab Golden Gate TALEN kit. This backbone is suitable for the TALEN-based generation of ...

.... These sequences were then cloned into an AAV backbone containing a minimal promoter to drive expression...

...Resources on Addgene.org Collection of empty plasmid backbones ...

...BioRender.com. Once DnaA breaks the local hydrogen bonds holding the DNA together, it recruits helicases ...

...interesting behaviors. There is diversity in the social bonds that they form including genetic mechanisms that...

...toxin-antitoxin system is MazE/MazF. The toxin MazF is a ribonuclease that cleaves specific sequences in RNA resulting...

...PMC41617. 7. Metzger, D, J Clifford, H Chiba, and P Chambon. 1995. “Conditional Site-Specific Recombination...

...with a protocol for a library of similar size and backbone.  Verification Once your library has been amplified...

...making it a more flexible cutter with the added bonus of low off-target effects. The SpG variant, discovered...

...differing amounts of target proteins or antibody bonding strengths.    . Steps of an IP: 1) Protein...

... type, but other envelopes such as the one from gibbon ape leukemia virus (GALV) are more stable (Sandrin...