We narrowed to 312 results for: bon

1. Viral Production at Addgene

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   ...responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for...

2. Viral Vectors 101: Preparing Pooled Libraries

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   ...with a protocol for a library of similar size and backbone.  Verification Once your library has been amplified...

3. Antibodies 101: Immunoprecipitation

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   ...differing amounts of target proteins or antibody bonding strengths.    . Steps of an IP: 1) Protein...

4. Viral Vectors 101: Pseudotyping

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   ... type, but other envelopes such as the one from gibbon ape leukemia virus (GALV) are more stable (Sandrin...

5. CRISPR-based Genome Editing Systems For Editing in E. coli

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   ...oligos are required and the BsaI linearized pEcgRNA backbone can be prepared in large amounts and frozen for...

6. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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   ...interesting behaviors. There is diversity in the social bonds that they form including genetic mechanisms that...

7. Finding nucleic acids with SHERLOCK and DETECTR

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   ...O, Jinek M (2016) Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated...

8. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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   Blog Post

   ...toxin-antitoxin system is MazE/MazF. The toxin MazF is a ribonuclease that cleaves specific sequences in RNA resulting...

9. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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   ...PMC41617. 7. Metzger, D, J Clifford, H Chiba, and P Chambon. 1995. “Conditional Site-Specific Recombination...

10. Using Video to Share Your Science: We Share Science

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    ...rather than on the day-to-day research that is the backbone of science. Facebook is continuing its growth,...

11. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    ...making it a more flexible cutter with the added bonus of low off-target effects. The SpG variant, discovered...

12. Tips for CRISPR Gene Editing in Mice

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    ...for validating your selected gRNA’s, and has the bonus benefit of supplying you with primers for validating...

13. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post

    ...means that the promoter present in your plasmid backbone must to be compatible with the type of RNA that...

14. CRISPR 101: Homology Directed Repair

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    ...alleles using long ssDNA donors and CRISPR ribonucleoproteins. Quadros RM, et al. Genome Biol. 18(1); (...

15. Fluorescent Proteins 101: When GFP lets you down

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    ...the Fluorescent Protein Collection Find empty backbones for fluorescent protein fusions Browse the Fluorescent...

16. Adeno-associated Viruses (AAVs) for Genome Editing

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    ...devised methods to deliver CRISPR components as ribonucleoproteins (RNPs) that can be supplied separately from...

17. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    ...How to Design Your gRNA Delivering CRISPR Ribonucleoproteins Resources on Addgene.org Browse All CRISPR...

18. March for Science

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    ...Scientists' Views PolitiFact: EPA head Scott Pruitt says carbon dioxide is not 'primary contributor' to global ...

19. Harnessing Bacterial Toxins for Allelic Exchange

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    ...the desired mutation and excision of the vector backbone. This step requires “counter-selection,” meaning...

20. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    ...the fluorescent protein collection Find empty backbones for fluorescent protein fusions Browse the fluorescent...