We narrowed to 310 results for: bon

1. Antibodies 101: Immunoprecipitation

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   ...differing amounts of target proteins or antibody bonding strengths.    . Steps of an IP: 1) Protein...

2. Viral Vectors 101: Pseudotyping

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   ... type, but other envelopes such as the one from gibbon ape leukemia virus (GALV) are more stable (Sandrin...

3. CRISPR-based Genome Editing Systems For Editing in E. coli

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   ...oligos are required and the BsaI linearized pEcgRNA backbone can be prepared in large amounts and frozen for...

4. Plasmids 101: Shuttle Vectors

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   ...Resources on Addgene.org Collection of empty plasmid backbones ...

5. Plasmids 101: Stringent Regulation of Replication

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   ...BioRender.com. Once DnaA breaks the local hydrogen bonds holding the DNA together, it recruits helicases ...

6. Viral Production at Addgene

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   ...responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for...

7. Viral Vectors 101: Preparing Pooled Libraries

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   ...with a protocol for a library of similar size and backbone.  Verification Once your library has been amplified...

8. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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   ...making it a more flexible cutter with the added bonus of low off-target effects. The SpG variant, discovered...

9. Tips for CRISPR Gene Editing in Mice

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   ...for validating your selected gRNA’s, and has the bonus benefit of supplying you with primers for validating...

10. Plasmids 101: The Promoter Region – Let's Go!

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    ...means that the promoter present in your plasmid backbone must to be compatible with the type of RNA that...

11. Fluorescent Proteins 101: When GFP lets you down

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    ...the Fluorescent Protein Collection Find empty backbones for fluorescent protein fusions Browse the Fluorescent...

12. Adeno-associated Viruses (AAVs) for Genome Editing

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    ...devised methods to deliver CRISPR components as ribonucleoproteins (RNPs) that can be supplied separately from...

13. Harnessing Bacterial Toxins for Allelic Exchange

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    ...the desired mutation and excision of the vector backbone. This step requires “counter-selection,” meaning...

14. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    ...the fluorescent protein collection Find empty backbones for fluorescent protein fusions Browse the fluorescent...

15. The Fluorescent Vegetables in Aptamer Soup

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    ... Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein purification and characterization using RNA...

16. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    ...How to Design Your gRNA Delivering CRISPR Ribonucleoproteins Resources on Addgene.org Browse All CRISPR...

17. March for Science

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    ...Scientists' Views PolitiFact: EPA head Scott Pruitt says carbon dioxide is not 'primary contributor' to global ...

18. CRISPR 101: Homology Directed Repair

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    ...alleles using long ssDNA donors and CRISPR ribonucleoproteins. Quadros RM, et al. Genome Biol. 18(1); (...

19. Multiple Plasmids at a Low Price: Kits from Addgene

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    ...create the pCell-Free vectors, Gateway-compatible backbones that enable cell-free expression of proteins in...

20. 10 Steps to a Perfect Science Talk

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    ...hiring manager or host for topic advice. An added bonus to knowing your audience is that it can help you...