We narrowed to 275 results for: mel

1. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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   ...labs and institutions. For example, search for “Melina Fan” and you’ll be able to go directly to a page...

2. Simplify Cloning with in vivo Assembly

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   ...First design the template binding region, with a melting temperature (Tm) of around 60 °C for all primers...

3. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ...genome-wide negative selection screens with both A375 (melanoma) and AT29 (colon cancer) cells and assessed the...

4. Lentiviral Vector Uses and Overview

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   ... A, Soulier J, Larghero J, Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien S, Cartier N, Aubourg P, Fischer...

5. Cpf1: A New Tool for CRISPR Genome Editing

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   ...potentially overcome some of Cas9’s shortcomings - namely its blunt double stranded cleavage and G-rich PAM...

6. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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   ...excitation peak from 395 nm to 488 nm, effectively ameliorating the deficiencies found in the wildtype protein...

7. Transferable Skills Guide: External Collaborations

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   ...keep these jobs in mind! References 1. Sinche, Melanie, et al. "An evidence-based evaluation of transferrable...

8. Fluorescent Proteins 101: Aptamer Fluorophores

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   ...stable in cells. The new aptamer also has a higher melting temperature than its Spinach ancestors, and has...

9. Mesothelioma - Causes, Symptoms, and Treatment

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   ... mean that most mesothelioma patients have an extremely poor prognosis. Together, the cause, long latency...

10. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    ... to the video game character but rather to an extremely important gene in animal development. To understand...

11. Lab to Office Culture Shock

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    ...that at a desk, it is much easier to stick to timelines. This is probably because you don't have to worry...

12. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    ... syndrome gene therapy improves survival and ameliorates behavioral phenotypes in MeCP2 null (S51.002)...

13. To Codon Optimize or Not: That is the Question

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    ...gene of interest? Codon optimization has become extremely useful in expressing functional proteins in hosts...

14. New CRISPR Tools: Cas7-11 and PASTE

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    ...the bottom of Tokyo Bay. When a weird, sulfurous smelling, black pellet was sent to the AbuGoot lab for ...

15. How to Be an Excellent Trainee

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    ...group). The bench mentor-mentee relationship is extremely important – without a good set up it could be ...

16. Twenty Years of Sharing: Expanding Accessibility

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    ...navigated customs and arrived at their destination in a timely manner and at the correct temperature. To date,...

17. Antibodies 101: The Four ELISAs and When to Use Them

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    ...method are similar to those of the direct method. Namely, since the assay requires a single conjugated primary...

18. Plasmids 101: Stringent Regulation of Replication

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    ...DnaA to get the job done.   Figure 1: DNA melting by DnaA and IHF is the first step of replication...

19. Tips for Writing a Good Cover Letter

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    ...of your publications on [specific topic]. I am extremely interested in pursuing research in [specific topic...

20. CRISPR-based Genome Editing Systems For Editing in E. coli

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    ... E. coli B strains, 1 E. coli W strain, and 1 Tatumella citrea, with a total of 70 sites edited. We optimized...