AAVs in Retinal Gene Therapy
Type
Blog Post
...now, 2 decades later, is it finally back in the limelight. On December 19, 2017 the FDA approved Luxturna...
We narrowed to 279 results for: mel
-
-
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post...labs and institutions. For example, search for “Melina Fan” and you’ll be able to go directly to a page... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post...Resources References Hwang, J. M., Piccinini, T. E., Lammel, C. J., Hadley, W. K., & Brooks, G. F. (1986). ... -
An Introduction to Adenovirus
TypeBlog Post...targeted cells is less likely for adenoviral vectors – namely due to the removal of certain adenoviral genes ... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...have their own unusual sort of virulence as well — namely that they can cause cancer via insertional mutagenesis... -
Getting to Know Expansion Microscopy
TypeBlog Post...17523-8 Prakash K, Diederich B, Heintzmann R, Schermelleh L (2022) Super-resolution microscopy: a brief... -
Lentiviral Vector Uses and Overview
TypeBlog Post... A, Soulier J, Larghero J, Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien S, Cartier N, Aubourg P, Fischer... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post...potentially overcome some of Cas9’s shortcomings - namely its blunt double stranded cleavage and G-rich PAM... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...excitation peak from 395 nm to 488 nm, effectively ameliorating the deficiencies found in the wildtype protein... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...navigated customs and arrived at their destination in a timely manner and at the correct temperature. To date,... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog Post...method are similar to those of the direct method. Namely, since the assay requires a single conjugated primary... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog Post...DnaA to get the job done. Figure 1: DNA melting by DnaA and IHF is the first step of replication... -
Tips for Writing a Good Cover Letter
TypeBlog Post...of your publications on [specific topic]. I am extremely interested in pursuing research in [specific topic... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post...stable in cells. The new aptamer also has a higher melting temperature than its Spinach ancestors, and has... -
Transferable Skills Guide: External Collaborations
TypeBlog Post...keep these jobs in mind! References 1. Sinche, Melanie, et al. "An evidence-based evaluation of transferrable... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog Post... to the video game character but rather to an extremely important gene in animal development. To understand... -
How to Be an Excellent Trainee
TypeBlog Post...group). The bench mentor-mentee relationship is extremely important – without a good set up it could be ... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog Post... syndrome gene therapy improves survival and ameliorates behavioral phenotypes in MeCP2 null (S51.002)... -
To Codon Optimize or Not: That is the Question
TypeBlog Post...gene of interest? Codon optimization has become extremely useful in expressing functional proteins in hosts... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog Post... E. coli B strains, 1 E. coli W strain, and 1 Tatumella citrea, with a total of 70 sites edited. We optimized...