We narrowed to 277 results for: mel

1. Viral Vectors 101: The Retroviral Lifecycle

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   ...have their own unusual sort of virulence as well — namely that they can cause cancer via insertional mutagenesis...

2. Getting to Know Expansion Microscopy

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   ...17523-8 Prakash K, Diederich B, Heintzmann R, Schermelleh L (2022) Super-resolution microscopy: a brief...

3. Simplify Cloning with in vivo Assembly

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   ...First design the template binding region, with a melting temperature (Tm) of around 60 °C for all primers...

4. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ...genome-wide negative selection screens with both A375 (melanoma) and AT29 (colon cancer) cells and assessed the...

5. AAVs in Retinal Gene Therapy

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   ...now, 2 decades later, is it finally back in the limelight. On December 19, 2017 the FDA approved Luxturna...

6. Lentiviral Vector Uses and Overview

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   ... A, Soulier J, Larghero J, Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien S, Cartier N, Aubourg P, Fischer...

7. Cpf1: A New Tool for CRISPR Genome Editing

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   ...potentially overcome some of Cas9’s shortcomings - namely its blunt double stranded cleavage and G-rich PAM...

8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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   ...excitation peak from 395 nm to 488 nm, effectively ameliorating the deficiencies found in the wildtype protein...

9. Rett Syndrome: A History of Research and Therapeutic Outlooks

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   ... syndrome gene therapy improves survival and ameliorates behavioral phenotypes in MeCP2 null (S51.002)...

10. To Codon Optimize or Not: That is the Question

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    ...gene of interest? Codon optimization has become extremely useful in expressing functional proteins in hosts...

11. New CRISPR Tools: Cas7-11 and PASTE

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    ...the bottom of Tokyo Bay. When a weird, sulfurous smelling, black pellet was sent to the AbuGoot lab for ...

12. CRISPR-based Genome Editing Systems For Editing in E. coli

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    ... E. coli B strains, 1 E. coli W strain, and 1 Tatumella citrea, with a total of 70 sites edited. We optimized...

13. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    ... to the video game character but rather to an extremely important gene in animal development. To understand...

14. Lab to Office Culture Shock

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    ...that at a desk, it is much easier to stick to timelines. This is probably because you don't have to worry...

15. Transferable Skills Guide: External Collaborations

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    ...keep these jobs in mind! References 1. Sinche, Melanie, et al. "An evidence-based evaluation of transferrable...

16. Twenty Years of Sharing: Expanding Accessibility

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    ...navigated customs and arrived at their destination in a timely manner and at the correct temperature. To date,...

17. Fluorescent Proteins 101: Aptamer Fluorophores

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    ...stable in cells. The new aptamer also has a higher melting temperature than its Spinach ancestors, and has...

18. Mesothelioma - Causes, Symptoms, and Treatment

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    ... mean that most mesothelioma patients have an extremely poor prognosis. Together, the cause, long latency...

19. Using Video to Share Your Science: We Share Science

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    ...  “Singing” Fish Rely on Circadian Rhythm and Melatonin for the Timing of Nocturnal Courtship Vocalization...

20. How to Be an Excellent Trainee

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    ...group). The bench mentor-mentee relationship is extremely important – without a good set up it could be ...