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We narrowed to 578 results for: multi

Showing: 201 - 220 of 578 results

  1. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...in freezers that may be shared with multiple people or even multiple labs. Careful tracking prevents us... These barcodes additionally allow us to store multiple types of tube for each plasmid we have at Addgene...detail about that location as possible. Are there multiple freezers that the samples can be stored in? Different...

  2. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...quantitation without compromising sensitivity. To detect multiple targets in one reaction, Cas enzymes, such as ... Zika and a closely-related virus, Dengue from multiple sample sources. SHERLOCK could also detect low-frequency...Kellner MJ, Joung J, Collins JJ, Zhang F (2018) Multiplexed and portable nucleic acid detection platform ...(C2c2) Learn more about Cas12a (Cpf1) and its multiplex genome editing abilities Resources on Addgene.org...

  3. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...used to simultaneously target multiple genes, enabling efficient multiplexed genome editing in a single ...compact genome editors to date. We have developed multiple vector versions for TnpB-mediated genome editing...promising for delivery via viral vectors and for multiplexed gene editing applications. As research into TnpB...

  4. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ... size (aka your n value)" and should come from multiple, independent sources. We'll describe these a bit... of a technical control is the transfection of multiple separate wells (within the same plate) with purified...repeat your whole experiment from start to finish multiple times and on different days. Using our example...additional positive and negative controls, performing multiple replicates, and ultimately getting interpretable...

  5. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...electric potential that can be applied to the photomultiplier inside the cytometer to increase the electric...gate on single cells and exclude any events where multiple cells were clumped together. (B) Instead of FSC...refined gating strategy, I can, of course, also draw multiple individual gates around the populations of interest...) Instead of a quadrant gate, I am free to use multiple individual gates. Note that in this case, square...

  6. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...fluorescent protein. These were either (i) coded by the multiple cloning site and therefore consist of random amino...#85045). We often use restriction enzymes in the multiple cloning site for generating fusions. For the POI-fluorescent... acids (PVAT, when we use the AgeI site in the multiple cloning site) is sufficient. For the fluorescent... may guide design, it is advisable to generate multiple constructs with different insertions, since it...

  7. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...cloning required. As with other model systems, multiple gRNAs can be expressed to modify several genomic...genomic loci at once (get more information on multiplexing gRNAs here). Cas9 is commonly tagged with a nuclear...Zhang Y, Qi Y (2015) A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation...Resources on the Addgene Blog Read Up on CRISPR Multiplexing Tools Validate Your Genome Edit Design Your ...

  8. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...hotspot for dystrophin, exons 45-55, there are multiple common deletions that maintain the protein’s reading...skipping? For such a therapy to be successful, multiple requirements must be met. First, CRISPR would ...dystrophy. gRNAs can be designed to flank a single or multiple exons that, when deleted through NHEJ, will restore...Thakore PI, Majoros WH, Reddy TE, Gersbach CA. Multiplex CRISPR/Cas9-based genome editing for correction...

  9. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ..., you’ll run the same protein concentration in multiple lanes and cut up the membrane to treat each lane...are a little trickier than gels and may require multiple runs to optimize or troubleshoot.   Before you...protein gradient, compare your diluted samples. If multiple dilutions are showing the same signal (Figure ...also consider using polyclonal or recombinant multiclonal antibodies to increase overall signal from the...antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein not properly lysed Different...

  10. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ... extended to multiplexed probe sets that allow the simultaneous interrogation of multiple potential SNP...extends earlier studies through a high-sensitivity multiplexed format (21, 27). This methodology has been used...: PMC148763. 15. Pingle, Maneesh R., et al. "Multiplexed identification of blood-borne bacterial pathogens...

  11. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...inverted repeats (Figure 1). With this technique, multiple genes can be simultaneously silenced if a consensus...sequence is used (~90% identity between targets) or multiple target sequences are concatenated. hpRNA has been... robustly self-organize at the cellular and multicellular scale.     References Bezanilla M, Perroud P...J-F (2018) Engineering Artificial MicroRNAs for Multiplex Gene Silencing and Simplified Transgenic Screen...

  12. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...us about a new tool for efficient multiplex base editing and multiplex prime editing (requiring minimal...editing tool for large inserts.  DAP arrays for multiplex gene editing: Guest blogger Qichen Yuan tells ...

  13. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ... – the dreaded ‘undruggable’ proteins – having multiple hits or an entire pathway of possibilities greatly... complex diseases, it can aid in generation of multiple edits or implementation of expression systems ...candidates are selected.      CRISPR aids in multiple stages of the drug discovery pipeline.   ...

  14. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and ...Cpf1: A New Tool for CRISPR Genome Editing and Multiplex Genome Editing with CRISPR-Cpf1 Day 4: Edit validation...

  15. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...genome engineering tool offers flexibility, multiplexibility, and ease of use. In order for this technology...the tool’s specificity, orthogonality, and multiplexibility in various species and describe an assortment...from experts in the field, and enabling access to multiple plasmids used for various platform applications...

  17. Hot Plasmids - January 2023

    Type
    Blog Post
    ...only having one binding site per protein. Having multiple antibodies allows you to use them in parallel ...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:...  Multiple Authors...

  18. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...Massachusetts Medical School developed a dCas9-based multicolor labeling system to visualize chromosomal loci...plasmids at Addgene. References Ma, Hanhui et al. “Multicolor CRISPR labeling of chromosomal loci in human ...Central PMCID: PMC4364232. Ma, Hanhui et al. “Multiplexed labeling of genomic loci with dCas9 and engineered...

  19. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...resolution of this system are achieved by using multiple copies of the PP7 and MS2 coat protein binding...constant tracking. You can then directly monitor multiple rounds of translation from this single mRNA; you...cases, creating a reporter mRNA requires inserting multiple hairpin sequences into the coding sequence and...

  20. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ... cell research has prompted the development of multiple virus packaging methods. Across these methods,...sequence. This post was written and updated by multiple Addgenies. It was originally published in 2018...  Multiple Authors...
Showing: 201 - 220 of 578 results