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Showing: 201 - 220 of 381 results

  1. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...Phenol-chloroform extraction removes remaining contaminant proteins and RNase A from the DNA sample. When... This will help to remove proteins and other contaminants from the plasmid DNA. It is also advisable to...

  2. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...several micrograms of target DNA from just a few nanograms of template DNA through several cycles of denaturation...number of µl of dH 2 O equal to the number of nanomoles of DNA times 10. For example, if your lyophilized...

  3. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...then be exposed to a lysis buffer to release recombinant proteins directly onto the nitrocellulose for... 1 M NaCl in order to enhance folding of the recombinant proteins. Optimal salt concentration will vary...

  4. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...microinject into blastocysts to be implanted in pseudopregnant female mice. If you obtained the desired chimeric...microinjection, i-GONAD is performed directly on pregnant female mice using a small incision that exposes...

  5. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...doi.org/10.1371/journal.pone.0076310 Damdindorj, L., Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa...Nonhuman Primates after Intramuscular Injection of Recombinant Adeno-Associated Virus. Journal of Virology, ...

  9. Antibodies 101: Validation

    Type
    Blog Post
    ...non-mutant line but no signal in the mutant line! Recombinant Strategies This approach is essentially the opposite...ready-to-use antibodies from Addgene Designing a recombinant validation experiment? Find plasmids here!  ...

  10. Kit Free RNA Extraction

    Type
    Protocol
    ...sample(s) using a spectrophotometer (such as a Nanodrop), agarose gel, or bioanalyzer. For more information...

  12. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...more reproducible/renewable reagents, such as recombinant or hybridoma-derived monoclonal antibodies. It...µg/mL (1/100). * = monoclonal antibody, ** = recombinant antibody. Figure adapted from Biddle et al., ...

  13. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from... plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check out these plasmids...

  14. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...phosphatase-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-...tool for use as a donor in FRET (fluorescence resonance energy transfer) and FLIM (Fluorescence Lifetime...

  15. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...AAV9 in the liver. AAV9-X1.1 effectively and predominantly transduces neurons across brain regions, with...throughout the non-human primate brain. Nature Nanotechnology, 1–11. https://doi.org/10.1038/s41565-023-01419...

  16. New Coomassie Protocol Video

    Type
    Blog Post
    ...latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies."  Hot tip! This protocol can easily...

  17. The 12 Days of CRISPR

    Type
    Blog Post
    ...prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th day of CRISPR...

  19. Deck the Lab 2022!

    Type
    Blog Post
    ...giant paper mache Krampus performing routine lab maintenance tasks. Hot tip: make sure your PI/facilities ...
Showing: 201 - 220 of 381 results