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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 388 results for: nans

Showing: 201 - 220 of 388 results

  1. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...Phenol-chloroform extraction removes remaining contaminant proteins and RNase A from the DNA sample. When... This will help to remove proteins and other contaminants from the plasmid DNA. It is also advisable to...

  2. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...several micrograms of target DNA from just a few nanograms of template DNA through several cycles of denaturation...number of µl of dH 2 O equal to the number of nanomoles of DNA times 10. For example, if your lyophilized...

  4. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...endogenous epitope tags, or the introduction of dominant mutations of a gene into the endogenous allele...generating human somatic cell gene knockouts using recombinant adeno‐associated viruses." Nucleic acids research...

  5. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ... scFvs. What is an scFv? scFVs are a type of recombinant antibody. They are ~25 kDa single polypeptides...coli Expression System in Producing Antibody Recombinant Fragments. IJMS 21:6324 . https://doi.org/10.3390...

  6. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...toolset IPI’s anti-integrin toolset comprises 26 recombinant antibodies (rAbs) either produced de novo using...an expanding collection of readily accessible recombinant antibodies and plasmids. To encourage collaboration...

  7. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...glnV44 λ- JM109   General cloning and plasmid maintenance, blue/white screening, partly restriction-deficient...(mrr-hsdRMS-mcrBC) Pir1    For cloning and maintenance of a plasmids with R6Kγ origin; contains a mutant...

  8. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...doi.org/10.1371/journal.pone.0076310 Damdindorj, L., Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa...Nonhuman Primates after Intramuscular Injection of Recombinant Adeno-Associated Virus. Journal of Virology, ...

  9. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...microinject into blastocysts to be implanted in pseudopregnant female mice. If you obtained the desired chimeric...microinjection, i-GONAD is performed directly on pregnant female mice using a small incision that exposes...

  10. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...then be exposed to a lysis buffer to release recombinant proteins directly onto the nitrocellulose for... 1 M NaCl in order to enhance folding of the recombinant proteins. Optimal salt concentration will vary...

  12. Kit Free RNA Extraction

    Type
    Protocol
    ...sample(s) using a spectrophotometer (such as a Nanodrop), agarose gel, or bioanalyzer. For more information...

  13. Antibodies 101: Validation

    Type
    Blog Post
    ...non-mutant line but no signal in the mutant line! Recombinant Strategies This approach is essentially the opposite...ready-to-use antibodies from Addgene Designing a recombinant validation experiment? Find plasmids here!  ...

  17. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...more reproducible/renewable reagents, such as recombinant or hybridoma-derived monoclonal antibodies. It...µg/mL (1/100). * = monoclonal antibody, ** = recombinant antibody. Figure adapted from Biddle et al., ...

  18. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from... plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check out these plasmids...

  19. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...phosphatase-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-...tool for use as a donor in FRET (fluorescence resonance energy transfer) and FLIM (Fluorescence Lifetime...

  20. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...AAV9 in the liver. AAV9-X1.1 effectively and predominantly transduces neurons across brain regions, with...throughout the non-human primate brain. Nature Nanotechnology, 1–11. https://doi.org/10.1038/s41565-023-01419...
Showing: 201 - 220 of 388 results