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We narrowed to 399 results for: nans

Showing: 201 - 220 of 399 results
  1. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...sure that the sample is pure and free of any contaminants that could affect the measurements. You'll need...Addgene's Ready-to-Use Antibody collection Addgene's Recombinant Antibody Plasmids collection  ...
  2. DNA Purification Without a Kit

    Type
    Blog Post
    ...into this mixture to absorb the nucleic acids. Containinants bind cellulose less tightly than nucleic acids...placing the dipstick into a wash solution removes contaminants while nucleic acids stick around. Finally, purified...
  3. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...fluorescent biosensors. Aptabeacons Fluorescence resonance energy transfer (FRET) technology has been studied...PMC4441765. 4. Guo, Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014...
  4. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Kalamegam, G., Ahmed, F., Abbas, M., Sait, K., Anfinan, N., Al-Wasiyah,  mohammad khalid, Huwait, E., ...Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies Introduction to Antibodies...
  5. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ... through Addgene. Anti-V5 [IPI-SV5-Pk1] is a recombinant version of the original V5 tag mouse monoclonal...different antibody-based applications.  IPI’s recombinant version is a fusion of mouse variable and rabbit...
  6. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...-2 research   By Ashley Waldron Ready-to-use recombinant antibodies were recently added to the Addgene...Image from Addgene. These antibodies are recombinant versions of mouse monoclonal antibodies generated...
  7. Kit Free RNA Extraction

    Type
    Protocol
    ...sample(s) using a spectrophotometer (such as a Nanodrop), agarose gel, or bioanalyzer. For more information...
  8. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Corporation 237172 EIF2AK2(252-551)-NanoLuc Fusion Vector EIF2AK2 NanoLuc CMV VWM disease Promega Corporation... Arrowsmith 211083 pNLF1-C_EIF2B2:1-351 EIF2B2 NanoLuc CMV VWM, CACH Cheryl Arrowsmith 211112 pBiT3.1-...Vector VCP Halo CMV ALS Promega Corporation 237148 NanoBRET Assay Vector, TREM2-HaloTag TREM2 Halo CMV Alzheimer's...
  9. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...endogenous epitope tags, or the introduction of dominant mutations of a gene into the endogenous allele...generating human somatic cell gene knockouts using recombinant adeno‐associated viruses." Nucleic acids research...
  10. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ... scFvs. What is an scFv? scFVs are a type of recombinant antibody. They are ~25 kDa single polypeptides...coli Expression System in Producing Antibody Recombinant Fragments. IJMS 21:6324 . https://doi.org/10.3390...
  11. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...toolset IPI’s anti-integrin toolset comprises 26 recombinant antibodies (rAbs) either produced de novo using...an expanding collection of readily accessible recombinant antibodies and plasmids. To encourage collaboration...
  12. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...glnV44 λ- JM109   General cloning and plasmid maintenance, blue/white screening, partly restriction-deficient...(mrr-hsdRMS-mcrBC) Pir1    For cloning and maintenance of a plasmids with R6Kγ origin; contains a mutant...
  13. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...microinject into blastocysts to be implanted in pseudopregnant female mice. If you obtained the desired chimeric...microinjection, i-GONAD is performed directly on pregnant female mice using a small incision that exposes...
  14. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...doi.org/10.1371/journal.pone.0076310 Damdindorj, L., Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa...Nonhuman Primates after Intramuscular Injection of Recombinant Adeno-Associated Virus. Journal of Virology, ...
  15. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...then be exposed to a lysis buffer to release recombinant proteins directly onto the nitrocellulose for... 1 M NaCl in order to enhance folding of the recombinant proteins. Optimal salt concentration will vary...
Showing: 201 - 220 of 399 results