...for plasmid. pXXXXX-XXXX Step 1: Backbone name Include the empty backbone name in your plasmid name. This...backbones, visit Vector Database. Step 2: Insert name  Include information about the insert in your plasmid name...contains a mutation or modification, this should be included in the plasmid name. Mutations are generally listed...

...species, antibody species, or whether the results include knockout data.    Figure 2: Search options in ...description of the experiment. If that data was included in a publication, you'll find that information... is easy!  Here at Addgene, we love all data, including negative data, which can help researchers save...

...the template. The PE3 system does just this by including an additional sgRNA. Using this sgRNA, the prime... number of unintended prime editing outcomes, including indels. Therefore, they engineered a dominant-...     ✓ ✓ reflects an innovation always included in the indicated tool. ~ reflects an innovation... strategies in our CRISPR guide. Some examples include: TwinPE: two prime editors make complementary ...

...per gene and includes ~10,000 gRNAs targeting intergenic regions. The libraries also include 2,400 gRNAs...recently deposited to Addgene and you’d like it to be included in the next What’s New in CRISPR blog post, please...

... to a post-academic career. This training was “included” with their tuition, so to speak.  Unfortunately...the development of priorities for pd|hub which include building capacity to identify the most effective...scientists, but it is essential that we integrate inclusive career readiness training in this educational ...

...White House honored 13 “Champions of Change,” including our recent guest Drew Endy, in celebration of ...trouble, even when all kinds of other factors, including journal impact factor, study topic, and the corresponding...review. 8)     That same issue of Science also included an online survey of scientists showing overwhelming...

...attracted much attention over the past few years, including an article in the BBC Wildlife Magazine. It is...people for years and have gathered much acclaim - including being featured in the National Geographic and ... are now taking place and gathering momentum, including in Cambridge and Bristol. If you’re a scientist...

...team reveal the mechanism behind its activity including an extensive RNA scaffold and a domain needed ...inhibition library from the Chong Zhong lab. Now, we’ve included another E. coli genome-wide inhibition library...recently deposited to Addgene and you’d like it to be included in the next What’s New in CRISPR blog post, please...

...don't read, you're doomed.” 2. Always be sure to include various positive and negative controls to “convince...entertainment, politics, K-12, etc.” Addgene's team includes many PhD scientists who have stepped away from...Blog Series   Our 3 PIs answer more questions, including: Advice for Choosing a Research Project Finding...

...replication, and fluorescent proteins that are already included in Snapgene’s extensive database. Snapgene also...plasmids are difficult to sequence by NGS. These can include GC-rich and repetitive regions. These regions require... same features annotated in our plasmid maps, including our most popular primers and certain restriction...

...straightforward in cases where the inserted DNA includes an antibiotic resistance marker. After transformation...grow. However, often times, the plasmid backbone includes an antibiotic resistance cassette and you aren...Gateway cloning takes advantage of this property by including ccdB on its destination plasmids. In the destination...

...years of study to come. Some Freshers Fairs can include fairground rides and other fun activities and even...society organises talks by prominent scientists, including Nobel Laureates, who speak about their work and...recreational and educational events throughout the year, including a Christmas Chemistry symposium and a career night...

...protease. The carboxyl-terminus of the fusion protein includes an AviTag and a 6X HisTag for affinity purification...purification (see plasmid map and cartoon below). Including biotin in your culture and inducing protein expression...binding kinetics using a multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences...

...for half of the trip. Preparations ahead of time included contacting YNP staff to arrange permits to visit...frozen after collection. Figure 1: Paul included a voltmeter on top of the battery box to monitor...route that would reach all of the remote areas, including one hot spring requiring an overnight hike in....

...thickness. The fibers are drawn with microelectronics included in them for downstream control – this is important...components are added - the ‘bells and whistles’ – including microscale light emitting diodes, thermal sensors...neuroscience tools into the brains of model organisms, including the Anikeeva lab’s work on a similar neural fiber...

...napkin for paper, a small group of scientists including Stanley Falkow, Stanley Cohen, Herbert Boyer, ...eBook. Some highlights of the 2nd edition eBook include: new chapters on genome engineering, cloning, and...PMCID: PMC427208. Novick, R P et al. “Uniform Nomenclature for Bacterial Plasmids: A Proposal.”Bacteriological...

...lifesaver when your research requires cell culture and include a counter, lab timer, dilution calculator, and ...quite helpful and provides a list of directions including which buffers to use and that indicates if a sequential...reagents you work with in experiments. Hence, I’m including this MSDS app. The Chemical Safety Data Sheets...

...protein, etc...).  There are many online guides – including an excellent series of posts by Joachim Goedhart...risk of inaccurate data submissions, so FPbase includes a versioning system that allows users to see when...improvement and elaboration.  Current projects include incorporation of biosensors, protein evolution ...

... planning to soon expand to other cell types, including more physiologically-relevant systems, to catch... viral proteins except for Nsp3 and Nsp16, and includes an additional catalytically dead version of the...the viral protease, Nsp5. Our set also includes a GFP construct as a control. For easier portability, ...

...structure and transparency of methods sections, including clear information about reagents, and why other...roles on review and primary research journals including Trends in Cell Biology, Cell, Molecular Cell, ...inception and launch of other Cell Press journals, including Cell Metabolism, Cell Reports and Joule. In September...