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Showing: 201 - 220 of 457 results
  1. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...Nakahara, K. (2011). Local and retrograde gene transfer into primate neuronal pathways via adeno-associated... H. (1979). Selective retrograde transsynaptic transfer of a protein, tetanus toxin, subsequent to its..., H.G., and Strick, P.L. (1989). Transneuronal transfer of herpes virus from peripheral nerves to cortex...
  2. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    ...Addgene's lab. We ship individual plasmids as transformed bacteria in stab culture format. Our lab team... and collaborations with institutions and tech transfer offices that facilitate MTAs. The great feedback...
  3. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    ...popular envelope and packaging plasmids as well as transfer plasmids. Download the orange lentivirus background...px or 1080 px).         Do you do a lot of transformations with the plasmids you receive from Addgene?...
  4. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ... name suggests, lentiCRISPR v2 is a lentiviral transfer vector used to deliver both Cas9 and your gRNA...metalloproteinase-9 secretion, and identifying lysine methyltransferase substrates. We’re incredibly happy that lentiCRISPR...
  5. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ... the snail, the larvae reach the eyestalk and transform them into a pulsating display imitating a moving...grasshoppers and crickets. When it’s ready to transform into an aquatic adult, it forces its insect host...
  6. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...product is introduced into the cell by direct transformation. A given insert will typically contain both...selectable gene product for isolation of successful transformants. Addgene distributes several ready-to-use, modular...
  7. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...pHlash, that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab . Characteristics...releases energy as a photon. The photon energy is transferred to the acceptor fluorophore, a circularly permuted...known to enhance gene expression) were then cotransfected with these constructs, providing the machinery... principle of bioluminescence resonance energy transfer (BRET), whereby photons emitted by a Renilla luciferase...
  8. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on...joyful that getting to the final step and the transformed bacteria refuse to grow. Eventual success was...
  9. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...encompasses millions of choices including pharma, tech transfer, management consulting, science communication,...where you want to go will help you focus on the transferable skills that you will need to get there. Additional...
  10. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...to modify it to improve yields. For example, transfecting the producer cell line with an expression vector...PMC3345986. Meliani, Amine, et al. "Enhanced liver gene transfer and evasion of preexisting humoral immunity with...
Showing: 201 - 220 of 457 results