We narrowed to 457 results for: nsf
-
TypeBlog Post...Nakahara, K. (2011). Local and retrograde gene transfer into primate neuronal pathways via adeno-associated... H. (1979). Selective retrograde transsynaptic transfer of a protein, tetanus toxin, subsequent to its..., H.G., and Strick, P.L. (1989). Transneuronal transfer of herpes virus from peripheral nerves to cortex...
-
500,000 Plasmids Shipped and Counting!
TypeBlog Post...Addgene's lab. We ship individual plasmids as transformed bacteria in stab culture format. Our lab team... and collaborations with institutions and tech transfer offices that facilitate MTAs. The great feedback... -
Download Science-Themed Zoom Backgrounds from Addgene
TypeBlog Post...popular envelope and packaging plasmids as well as transfer plasmids. Download the orange lentivirus background...px or 1080 px). Do you do a lot of transformations with the plasmids you receive from Addgene?... -
Your Top Requested Plasmid in 2015!
TypeBlog Post... name suggests, lentiCRISPR v2 is a lentiviral transfer vector used to deliver both Cas9 and your gRNA...metalloproteinase-9 secretion, and identifying lysine methyltransferase substrates. We’re incredibly happy that lentiCRISPR... -
Our Most Popular Blog Posts from 2019
TypeBlog Post.... Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection by Alyssa Cecchetelli... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog Post... the snail, the larvae reach the eyestalk and transform them into a pulsating display imitating a moving...grasshoppers and crickets. When it’s ready to transform into an aquatic adult, it forces its insect host... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog Post...then formulate a plan to further develop your transferable skills and reach your career goals. Although... What Makes a Good Manager Anyway Developing Transferable Skills During Science Training ... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog Post...details how to transfect CRISPR plasmids into MEL cells by electroporation, sort your transfected cells via... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog Post...product is introduced into the cell by direct transformation. A given insert will typically contain both...selectable gene product for isolation of successful transformants. Addgene distributes several ready-to-use, modular... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...pHlash, that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab . Characteristics...releases energy as a photon. The photon energy is transferred to the acceptor fluorophore, a circularly permuted...known to enhance gene expression) were then cotransfected with these constructs, providing the machinery... principle of bioluminescence resonance energy transfer (BRET), whereby photons emitted by a Renilla luciferase... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog Post...of University of Michigan says, “Addgene has transformed the way in which we share plasmids and other ...shipping logistics and facilitate all material transfer agreements, thereby freeing up time for our depositors... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog Post...includes an antibiotic resistance marker. After transformation, plate the cells on medium containing the corresponding...selection will select for cells that have been transformed with the plasmid backbone (with or without your... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...your gene of interest, all you have to do is co-transform pBLADEONLY. That way, a previously cloned gene...create these images, the scientists used a strain transformed with pBLADE expressing superfolder GFP instead... -
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation
TypeBlog Post...sequences is Proteobacteria - the most frequent transfer of genes is from Proteobacteria to mammalian expression...natural gene sequence is, the less likely it is transferred into another organism in the lab. Predicting ... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog Post...non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on...joyful that getting to the final step and the transformed bacteria refuse to grow. Eventual success was... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog Post...encompasses millions of choices including pharma, tech transfer, management consulting, science communication,...where you want to go will help you focus on the transferable skills that you will need to get there. Additional... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog Post...However, careers outside of the lab highlight “transferable” skills such as leadership, management, and ...Leadership Networking University technology transfer office positions Market analyses Patent... -
Trading Spaces - Visiting Scientist Edition
TypeBlog Post...remote collaborations Transferable Skills Guide: External collaborations Transferable Skills Guide: Teamwork... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...to modify it to improve yields. For example, transfecting the producer cell line with an expression vector...PMC3345986. Meliani, Amine, et al. "Enhanced liver gene transfer and evasion of preexisting humoral immunity with... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post... like gel electrophoresis to separate DNA or transforming cells with green fluorescent protein, these ... into a host cell (through a process called transformation), essentially giving the cell all the instructions...