We narrowed to 474 results for: organization Addgene

...want to pick a topic and have no one show up. At Addgene, we wanted to think more closely about the wealth...: Discussing next-generation sequencing at the Addgene bioinformatics journal club! What happens during...figure. Some follow the same figure by figure organization with a different person responsible for each...messaging but we as scientists often prefer data. At Addgene we rely on polls and surveys. A mix of closed (...most likely the primary drivers of frequency. At Addgene, we opted to start with one per month. When to ...journal clubs should be well planned and carefully organized. Most importantly, they should be regularly assessed...own journal club! Additional resources on the Addgene blog Read our blog posts centered around scientific...

... & Small Molecule Fluorophores. Addgene Blog. https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores... is now the most popular self-labeling tag in Addgene’s collection. CLIP-tag: Also in 2008, Kai Johnsson...Various Many Complicated   Applications Organic dyes are typically both brighter and more photostable...at a price, though. Purchasing lots and lots of organic dyes or specialized ligands is more expensive than...are the most popular self-labeling enzymes in Addgene’s collection, so you may be able to get a plasmid...ligands brings up bad memories of undergraduate organic chemistry lab, don’t worry – many SNAP-tag and ...ligand to use for your experiment, and explore Addgene's collection to find self-labeling fusion proteins...

.... Additional Addgene Genome Engineering Resources Learn more about CRISPR with Addgene's CRISPR Guide ...Escherichia coli is the most widely used bacterial organism due to its ease of use, speed of growth, and well...are also being used for their distinct features. Addgene distributes many plasmids that can help researchers...MalE, OmpA ID Plasmid Promoter Tags PI Additional Addgene Protein Purification Resources The Structural Genomics...localization to the periplasm. The pCri System (Addgene #1000000058) plasmids can be utilized for heterologous...and Pichia pastoris . The EcoFlex MoClo Toolkit (Addgene #1000000080) features a library of promoters and...Gate modular cloning (MoClo) for use in E. coli . Addgene Blog E. Coli Strains for Protein Expression Protein...

...analyst, I negotiate with global organizations that partner with Addgene. What are the most important issues...training in a variety of transferable skills. At Addgene, we've set out to fill this gap by highlighting...with your colleagues within and outside of your organization. In my non-academic roles, some examples of ...issues for our partners and how can Addgene work with them to ensure we create a win-win situation? Learning...Richard D. Lewis Additional resources on the Addgene blog More from the transferable skills guide: ...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ...

...’re bringing you a brand new third edition of Addgene’s CRISPR 101 eBook. This new edition of the eBook...Genome Editing: Modifying the DNA sequence of an organism’s genome usually for a research or applied goal...CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA, a nuclease, or both. Applications...

... also find information on using Addgene’s website, the latest Addgene news, and awards that we offer there...Say hello to the new Addgene blog! Same URL, same great articles to help you with your experiments, but...with over 600 articles, we needed a better way to organize and present the vast collection of material so...articles you want to find without even leaving the Addgene blog! Just click on the search bar at the top of...Be the first to know about new articles on the Addgene blog by subscribing to instant alerts below.   ...author pages, where you can read more about the Addgenie behind the blog post and find more of their work...interview. Get in touch by emailing us at blog@addgene.org or tell us in the comments below. We’ll track...

... a wide range of organisms and cell lines. In C. elegans, one of the model organisms which I use to study...perform pha-1 co-conversion are available through Addgene. In 2019, Farboud et al. described another similar...To see more CRISPR tools for C. elegans, visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives...methods – could greatly streamline editing in other organisms and systems, allowing rapid progress in a wide...about genome editing with CRISPRs? Check-out Addgene's CRISPR Resources Learn about gRNA design and...

...culture that will hopefully help you keep a well organized lab and contamination free environment for successful...many times and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage...consistent growth rate. For every minute spent organizing, an hour is earned (Benjamin Franklin)! It’s ...Second  Edition), 2012. Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian...should keep in mind that UV light only kills microorganisms that it strikes directly. UV light exposure..."Ostreopsis cf. ovata induces cytoskeletal disorganization, apoptosis, and gene expression disregulation...Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your research Find...

...fluorescent when ectopically expressed in most organisms. Furthermore, fusing FPs to another protein usually...Additionally, many other FPs have been found in other organisms. Blue 424 - 467 nm Cyan 474 - 492 nm Green...More examples of these biosensors can be found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be...More Helpful Websites & Resources: FP guide at Addgene Interactive Visualization of Fluorescent Protein...

...generate these lines have been deposited here with Addgene for distribution to the research community. With... Endogenous Gene Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human... with endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure.../Cas9 in human stem cells to illuminate cell organization. Mol Biol Cell. 28 (21), 2854–2874. https://...

...experiment? What if after receiving the plasmid from Addgene you could directly start your digest, PCR, or transformation...collection is highly diverse in terms of target organisms (from bacteria to mammals), cloning techniques...Suggestion Form.  Additional resources on the Addgene blog Browse blog posts from our Plasmids 101 blog...the various ways to quantify DNA Resources on Addgene.org Learn about our other beta projects Read our...

...Developing Addgene’s Brand and culture Chapter 7 - Building Addgene as a global organization (now with ...Resources from the Addgene Blog Addgene's a nonprofit? Get an Inside Look at Addgene's NGS Quality Control... In this episode of the Addgene podcast, we sit down with Addgene’s Founders - Melina Fan, Ken Fan, and...interview with Addgene's founders Podcast breakdown Intro Chapter 1 - Who are Addgene's Founders? Chapter...Chapter 2 - The process of founding Addgene, where the idea for Addgene came from and how the idea got off...current roles at Addgene and beyond - Ginkgo, Tufts Launchpad, and more Chapter 10 - Addgene's Future, the ...efforts at Addgene as the Chief Financial Officer and Chief Operating Officer. Prior to Addgene, Kenneth...

...Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s incredibly important for scientific...distribution and disseminate knowledge around the globe. Addgene’s progress in international distribution As you ...are making great progress toward spreading the Addgene community into developing regions like South America...the countries that have requested plasmids from Addgene since our inception in 2004. In the near future...to more collaborations with Seeding Labs and organizations like it. Additional Resources Find out how...SeedingLabs or on Facebook Additional Resources on the Addgene Blog Learn About Our Recent Visit to China Read... Institutions that Deposit their Plasmids with Addgene ...

...shipping look like at Addgene? Here's an inside peak into the daily workings in Addgene's lab. We ship individual...March 26, 2015. How does Addgene make it happen? Our awesome team of Addgenies! Our team recently grew ...Yesterday we hit a huge milestone here at Addgene – 500,000 plasmids shipped! That's a 1/2 million plasmids...labeling stabs with barcodes as they go. Stabs are organized by shipment and moved to our packing area.Using...with shipping labels and paperwork. Our stacks of Addgene boxes are now ready to be sent around the world... comments from our blog readers. You all drive Addgene to succeed and to improve, so thank you. 500,000x... to necessitate an office move. We've got ~50 Addgenies working to ship plasmids, analyze QC sequence,...

...sea anemone (Chan et al., 2006). Why do these organisms have chromoproteins? One reason may be that chromoproteins...symbiotic algae, during heat stress. For other organisms, like the sea anemone, scientists think that chromoproteins...art, let us know! Additional resources on the Addgene blog Find our Fluorescent Protein 101 blog series... for making plasmid constructs Resources on Addgene.org Find fluorescent proteins for your research ...

... produce all of the differentiated cells in an organism, including the embryonic and extraembryonic cell...quantities in specific “stem cell niches” found in many organs in the adult human body. These generate specialized...PMCID: PMC3347549. Additional Resources on the Addgene Blog pCXLE Toolkit Stem Cell Models for Disease...Differentiate the Stem Cell Field Resources on Addgene.org Visit Our Stem Cell Page Find Plasmids for Reprogramming...

... 2. Determining the spatial and hierarchical organization of chromatin interaction networks  A second ...techniques: the powerful tools for spatial genome organization deciphering. Molecular Cytogenetics, 11. https...s13059-020-01973-w Additional Resources on the Addgene Blog First time CRISPR user? Check out our tips... Here Brush Up on Your CRISPR Background with Addgene’s CRISPR Guide Page ... Favorite CREs Here Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic...

...plant tissue cultures.   C. roseus is not a model organism such as Arabidopsis or tobacco. Studying gene ...transgenic plants is a common problem in non-model organisms. Therefore, transient methods are usually the ...Nicola Patron, who shared the MoClo plant kit via Addgene. For us, transient leaf transformations are a great...PMCID: PMC2699436. Additional resources on the Addgene blog Read all of our plant biology blog posts ...Read our Plasmids 101 blog posts Resources on Addgene.org Browse our Plant Plasmids and Resources page...

...become a positive force for public health. Visit Addgene's Microbiology Pages What is antibiotic resistance... that the drug prevents the growth of a living organism. As for multi-drug resistance: definitions vary...vary in the literature, but generally organisms with multi-drug resistance are resistant to three or more...Resistance Fact Sheet  Additional Resources on the Addgene Blog Learn How Antibiotic Resistance Genes Are...is an intense amount of competition between microorganisms for available resources. This competition drives...Our Microbiology Pages Additional Resouces on Addgene.org Check Out Our Microbiology Pages ...

...their suppliers, and public health and medical organizations are working to help educate the public about...chance of an opportunistic infection like for organisms like C. difficile. In 2014, Citorik et al. and...PMC4˚237163. Find plasmids from this paper at Addgene. 2. Bikard, David, et al. “Exploiting CRISPR-Cas...PubMed: PMID 26973885. Additional Resources on the Addgene Blog CRISPR Methods for Bacterial Genome Engineering... out of the Genome  Additional Resources on Addgene.org Find CRISPR Plasmids for Your Research Read ...