...sea anemone (Chan et al., 2006). Why do these organisms have chromoproteins? One reason may be that chromoproteins...symbiotic algae, during heat stress. For other organisms, like the sea anemone, scientists think that chromoproteins...art, let us know! Additional resources on the Addgene blog Find our Fluorescent Protein 101 blog series... for making plasmid constructs Resources on Addgene.org Find fluorescent proteins for your research ...

...plant tissue cultures.   C. roseus is not a model organism such as Arabidopsis or tobacco. Studying gene ...transgenic plants is a common problem in non-model organisms. Therefore, transient methods are usually the ...Nicola Patron, who shared the MoClo plant kit via Addgene. For us, transient leaf transformations are a great...PMCID: PMC2699436. Additional resources on the Addgene blog Read all of our plant biology blog posts ...Read our Plasmids 101 blog posts Resources on Addgene.org Browse our Plant Plasmids and Resources page...

... 2. Determining the spatial and hierarchical organization of chromatin interaction networks  A second ...techniques: the powerful tools for spatial genome organization deciphering. Molecular Cytogenetics, 11. https...s13059-020-01973-w Additional Resources on the Addgene Blog First time CRISPR user? Check out our tips... Here Brush Up on Your CRISPR Background with Addgene’s CRISPR Guide Page ... Favorite CREs Here Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic...

...become a positive force for public health. Visit Addgene's Microbiology Pages What is antibiotic resistance... that the drug prevents the growth of a living organism. As for multi-drug resistance: definitions vary...vary in the literature, but generally organisms with multi-drug resistance are resistant to three or more...Resistance Fact Sheet  Additional Resources on the Addgene Blog Learn How Antibiotic Resistance Genes Are...is an intense amount of competition between microorganisms for available resources. This competition drives...Our Microbiology Pages Additional Resouces on Addgene.org Check Out Our Microbiology Pages ...

...their suppliers, and public health and medical organizations are working to help educate the public about...chance of an opportunistic infection like for organisms like C. difficile. In 2014, Citorik et al. and...PMC4˚237163. Find plasmids from this paper at Addgene. 2. Bikard, David, et al. “Exploiting CRISPR-Cas...PubMed: PMID 26973885. Additional Resources on the Addgene Blog CRISPR Methods for Bacterial Genome Engineering... out of the Genome  Additional Resources on Addgene.org Find CRISPR Plasmids for Your Research Read ...

...the membranes into stacked structures including organized smooth endoplasmic reticulum (OSER) whorls. 2)...distorted into dense stacked membranes termed Organized Smooth Endoplasmic Reticulum or OSER if a dimerizing...can be monomerized with an A206K mutation [2]. Addgene has many of these constructs and they are designated...PMCID: PMC3324619. Additional Resources on the Addgene Blog Read Professor Snapp's Post on mOX FPs Find...in 720°, i.e. they are on a membrane or in an organelle, then their effective concentration will be much...in Your Experiments Additional Resources on Addgene.org Browse our Fluorescent Protein Resources Find...

...default backgrounds all year and want a change? Addgene has you covered. Today we’re releasing 11 bold ...reading this blog, chances are you are a fan of Addgene or our very cute mascots!  Blugene has been helping...might want to use one of our virus backgrounds. Addgene’s viral service provides ready-to-use AAV and lentiviral...the joke? If not, you might want to check out Addgene’s Fluorescent Proteins blog topic to learn more ...background.     You can find all of the plasmids that Addgene offers by visiting our collections page. Here, ..., you can browse plasmids by field, organism, research technique, and more. Download the green DNA background...background.     Since we ❤️ DNA at Addgene, we're presenting you with another DNA background option, Download...

...shipping look like at Addgene? Here's an inside peak into the daily workings in Addgene's lab. We ship individual... all drive Addgene to succeed and to improve, so thank you. 500,000x Thank You! Addgene's new location...March 26, 2015. How does Addgene make it happen? Our awesome team of Addgenies! Our team recently grew ...Yesterday we hit a huge milestone here at Addgene – 500,000 plasmids shipped! That's a 1/2 million plasmids...labeling stabs with barcodes as they go. Stabs are organized by shipment and moved to our packing area.Using...with shipping labels and paperwork. Our stacks of Addgene boxes are now ready to be sent around the world... to necessitate an office move. We've got ~50 Addgenies working to ship plasmids, analyze QC sequence,...

...the United States but around the world. As an organization that directly collaborates with NIH-funded researchers...utilizes grants to support our own initiatives, Addgene is deeply concerned about these developments. These... We Are Here to Help Despite these challenges, Addgene remains steadfast in our mission to accelerate ...vital during this period of constrained funding. Addgene is uniquely positioned to help research institutions...Universities and research institutions can turn to Addgene as a trusted partner for archiving their biological...scientific progress depends on our collective response. Addgene stands ready to collaborate with partners across...work and what support would be most valuable from Addgene during this time. Your input will directly shape...

...quietly learning about Addgene’s past and present, but the real promise of Addgene lies in our future. As...every aspect of our organization. One thing that will remain the same is Addgene’s commitment to supporting...Addgene saw big changes in 2022, from the launch of a new material to the redesign of a community resource...number of people. I look forward to working with Addgene colleagues, collaborators, partners, and the larger...

...generated using random genomic fragments from the organism of interest, or a defined cDNA library (e.g. the...number of two-hybrid systems available in model organisms, including bacteria, alternative fungi (C. albicans... Pubmed Central PMCID: PMC514463. Resources At Addgene Browse Our Yeast Expression Vectors Find Bacterial...

...specimens and for in vivo imaging of transparent organisms. Advances in tissue clearing Similar to light ...3DISCO (3-dimensional imaging of solvent cleared organs) method of imaging rodent brains and spinal cords...).  These viral plasmids are available through Addgene.  The combination of LSFM with multiple tissue ...

...your host organism. Because transcription machinery differs between cell types or organisms, promoters...writing of this article. Additional Resources on the Addgene Blog Browse All Plasmids 101 Posts Learn about...GFP Resources on Addgene.org Find Plasmids for Your Research     ...  Kendall Morgan...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...discussion, we bring up a performance Ben and Niroshi organized for Tyler's (the host's) thesis defense. This ...Additional Resources on the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research...Process  Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your...

...for Scientists Resources on Addgene.org Learn about Careers at Addgene ...accordingly. Read Additional Career Posts on the Addgene Blog How did this change in labor laws come about... salaries (which have been made by multiple organizations across academia). Increased postdoc wages, overtime... salaries as their website implies. These 5 organizations, according to the NSF data, account for 18% ...Research @FORsymp.   Additional Resources on the Addgene Blog Read about the 2015 and 2016 Future of Research...

...region (approximately 500bp is best) and select the organism. It will then tell you the available gRNA targets...can also find many previously validated gRNAs at Addgene. In vitro screening for the best gRNA Figure...Find Plasmids for Testing Your gRNAs in vitro at Addgene CRISPR/Cas9 gene editing in mice Now that you’ve... much more from our favourite mammalian model organisms in the near future.  Many thanks to our guest...PMCID: PMC3969854. Additional Resources on the Addgene Blog Get the Basics of Mouse Genome Engineering...Homology Directed Repair Additional Resources on Addgene.org Find Software Tools to Help Design You gRNA ...

...Blotting Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based technique, validation...you are confident, especially if you work in an organism that is underrepresented in the biomedical research...10.1038/nmeth.3995 Additional Resources on the Addgene blog Learn about research applications of antibodies...

...and stably maintain the genes they carry among organisms belonging to many different phylogenetic groups...bacteria and express proteins within different host organisms. pBBR1-based plasmids and plasmids in incompatibility...  Find broad host range recombinant vectors at Addgene!   References and resources References Doran, ...10.1007/s13205-023-03507-0  More resources on the Addgene blog Plasmids 101: Origin of Replication Plasmids...

...over-express coding sequences from a given cell type or organism, have been used in a similar manner to Cas9 activators...lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3) and organisms (Chavez et al., 2016). To learn more about the...    Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board. His laboratory at Columbia...doi.org/10.1038/nbt.3149 Additional Resources on the Addgene Blog Learn how to Choose the Best Cas9 Variant... CRISPR Has Been Used with AAV Resources on Addgene.org Find Cas9 Activators Find Cas9 Repressors Catch...

...plasmids with Addgene. Additional control plasmids for the TKO library will be available at Addgene soon. Hart...cellular compartments (cytosol, Addgene catalog #71245; mitochondria, Addgene catalog #71247) Rosella provides... in vitro cell systems and diverse model organisms. Addgene’s newest TALEN kit, the FusX assembly system...read? Listen to our hot plasmids segment on the Addgene Podcast. View Plasmids from:  March (Q1) | June...sequencing. The STARR-seq backbone is available from Addgene in both human and fly versions. Arnold, et al....alongside a growing number of CRISPR libraries from Addgene’s repository that can be used to perform screens...PMID: 24395366 Oxford Drosophila CRISPR library Addgene has had the privilege of distributing several human...