Addgene

We narrowed to 299 results for: pET

Showing: 201 - 220 of 299 results

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PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Type

Blog Post

.... M., Pallaseni, A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante, F. G., & Parts, L. (2023). Prediction...
Tips for a 1st time AAV user (by a Rookie AAV user)

Type

Blog Post

...bacterial strains, like NEB Stable. NEB Stable competent cells lack RecA, a protein that aids in the homologous...
PCR Applications for Copy Number Variation Assays

Type

Blog Post

... RQ value of 1.5 means you have either made a pipetting error or an incredible discovery. The range of...
Course-Based Undergraduate Research Experiences (CUREs)

Type

Blog Post

...Einstein Discovers America. New York./ Callahan, K., Peterson, C., et al. 2022. External collaboration results...
CRISPR 101: Making a Knock-In Cell Line

Type

Blog Post

... al., Nat Com). Fig. 3: HDR events compete with NHEJ-mediated edits of small insertions and...
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Type

Blog Post

...been full of dark matter: enhancers, lncRNAs, repetitive elements, repressors, insulators, and more. We...
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Type

Blog Post

.... "Minigenomes, transcription and replication competent virus-like particles and beyond: reverse genetics...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

...that inhibit BiFC in your cellular system. BiFC competition analysis can be used in lieu of negative controls...
DNA Purification Without a Kit

Type

Blog Post

...mammalian cells in 30 seconds or less without using a pipette. Here’s how this method works: nucleic acids are...
A Guide to Starting Your Own Journal Club

Type

Blog Post

...literature and emerging technologies to remain competitive and relevant. Enter: the journal club. A journal...
MXS Chaining

Type

Blog Post

...for the modular assembly of highly similar or repetitive sequences. Intermediate chaining products (called...
Summer SciComm Series: Modes of Communication

Type

Blog Post

... needed. Traditional media formats are quite competitive while digital can be oversaturated. Transition...
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

Type

Blog Post

...survive as a researcher because of the tough competition for academic positions and poor grant situation...
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

Type

Blog Post

...on OWW, including those for preparing your own competent cells. If you’re familiar with the process of ...
Getting the Most from Your Lentiviral Transduction

Type

Blog Post

...ml before the media becomes cloudy. Mycoplasma compete with host cells for nutrients and can alter expression...
The PAM Requirement and Expanding CRISPR Beyond SpCas9

Type

Blog Post

...Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered...
Hot Plasmids - August 2020

Type

Blog Post

...plasmids were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate...
Plasmids 101: Stringent Regulation of Replication

Type

Blog Post

...number control of IncIalpha plasmid ColIb-P9 by competition between pseudoknot formation and antisense RNA...
Designing Your Chalk Talk for the Academic Job Interview

Type

Blog Post

...robust research proposal will distinguish between competing models. Comparing model A vs. B is more constructive...
Lab to Office Culture Shock

Type

Blog Post

...Finally, if you still find the idea of not holding a pipette again upsetting, there are ways to get back to ...

Showing: 201 - 220 of 299 results

Results per page:

PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Tips for a 1st time AAV user (by a Rookie AAV user)

PCR Applications for Copy Number Variation Assays

Course-Based Undergraduate Research Experiences (CUREs)

CRISPR 101: Making a Knock-In Cell Line

CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

DNA Purification Without a Kit

A Guide to Starting Your Own Journal Club

MXS Chaining

Summer SciComm Series: Modes of Communication

Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

Getting the Most from Your Lentiviral Transduction

The PAM Requirement and Expanding CRISPR Beyond SpCas9

Hot Plasmids - August 2020

Plasmids 101: Stringent Regulation of Replication

Designing Your Chalk Talk for the Academic Job Interview

Lab to Office Culture Shock

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