We narrowed to 303 results for: pET

1. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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   Blog Post

   ...attend conferences makes it very challenging to compete in today’s tough academic or industry job market...

2. Tips for a 1st time AAV user (by a Rookie AAV user)

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   Blog Post

   ...bacterial strains, like NEB Stable. NEB Stable competent cells lack RecA, a protein that aids in the homologous...

3. PCR Applications for Copy Number Variation Assays

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   Blog Post

   ... RQ value of 1.5 means you have either made a pipetting error or an incredible discovery. The range of...

4. Degrading DNA with Cascade-Cas3

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   Blog Post

   ...References Loeff, L., Brouns, S. J., & Joo, C. (2018). Repetitive DNA reeling by the Cascade-Cas3 complex in nucleotide...

5. Common Injection Routes in Mice

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   Blog Post

   ... is easier, less invasive, and only requires a pipette. Since the substances are administered through ...

6. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   Blog Post

   .... M., Pallaseni, A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante, F. G., & Parts, L. (2023). Prediction...

7. Google Forums Round Up: First Impressions of NgAgo

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   Blog Post

   ...blogs, or in the comments section below. Keep pipetting and happy genome editing! Check Out Other Argonaute...

8. MXS Chaining

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   Blog Post

   ...for the modular assembly of highly similar or repetitive sequences. Intermediate chaining products (called...

9. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   Blog Post

   ...that inhibit BiFC in your cellular system. BiFC competition analysis can be used in lieu of negative controls...

10. Summer SciComm Series: Modes of Communication

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    Blog Post

    ... needed. Traditional media formats are quite competitive while digital can be oversaturated. Transition...

11. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    Blog Post

    ...protocol. Are any of your materials expired? Did you pipette carefully? Remember to discard flow-through solutions...

12. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post

    ...Einstein Discovers America. New York./  Callahan, K., Peterson, C., et al. 2022. External collaboration results...

13. CRISPR 101: Making a Knock-In Cell Line

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    Blog Post

    ... al., Nat Com).     Fig. 3: HDR events compete with NHEJ-mediated edits of small insertions and...

14. DNA Purification Without a Kit

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    Blog Post

    ...mammalian cells in 30 seconds or less without using a pipette.  Here’s how this method works: nucleic acids are...

15. A Guide to Starting Your Own Journal Club

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    Blog Post

    ...literature and emerging technologies to remain competitive and relevant. Enter: the journal club.  A journal...

16. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    Blog Post

    ...been full of dark matter: enhancers, lncRNAs, repetitive elements, repressors, insulators, and more. We...

17. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    Blog Post

    .... "Minigenomes, transcription and replication competent virus-like particles and beyond: reverse genetics...

18. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    Blog Post

    ...survive as a researcher because of the tough competition for academic positions and poor grant situation...

19. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post

    ... assortment toxins to claim territory or kill competing microorganisms, but did you know bacteria also...

20. Plasmids 101: Codon usage bias

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    Blog Post

    ... abundance relative to cognate tRNA abundance Repetitive sequences Restriction sites Sequences prone to...