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  3. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ... of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful? The power of the FLEx system...for clearer characterization of the location, morphology, and circuit mapping of a targeted neuronal population...

  4. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...responses of cryptochrome proteins or organic fluorophores (Karki et al., 2021; Lee et al., 2011; Matsuoka..., & Zoltowski, B. D. (2021). Cryptochromes: Photochemical and structural insight into magnetoreception...

  5. Pooled Library Amplifications

    Type
    Blog Post
    ...Screening Rinehart Lab Iterative Synthetically Phosphorylated Isomers (iSPI) Libraries ~11,000–110,139 ...

  6. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...commonly used promoters include the phosphate and copper inducible PHO5 and CUP1 promoters respectively. ...

  10. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ... This process can be avoided direct antibody fluorophore conjugates are used, but these are usually more...secondary nanobodies conjugated with the desired fluorophores. In figure 4B below, triple colocalization is...

  12. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...your samples and maintaining cellular and tissue morphology. Formaldehyde is the most commonly used cross-linker...reagents - cross-linkers better preserve sample morphology, while organic solvents can reveal otherwise ...

  13. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...basic science research (neuronal tracing and morphologic studies), as well as translational applications...capsids are useful tools for neuronal tracing and morphology studies when used as part of a two part fluorescent...

  15. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...including a lot of future Addgenies as shown in the photo below.  Kids and adults alike enjoyed some friendly...of your plans by email, gong, evite, posters, megaphone, or your company’s intranet. Don’t forget about...

  16. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...γ-Retrovirus Guide Protocols Agarose Gel Electrophoresis Bacterial Transformation How to Design a Primer... How to Design Primers for PCR Agarose Gel Electrophoresis Inoculating Liquid Bacterial Cultures Restriction...

  17. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...-I3, while initially dimmer, showed greater photostability and also matured considerably faster, with ...the intestine. Figure 3: Expression of fluorophore-tagged GOLG-4 through eight stages of embryonic...

  20. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...protein. VSV-G is a trimeric protein that binds phosphatidylserine and low-density lipoprotein receptors (LDLRs...in sera and augmented transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman...
Showing: 201 - 220 of 396 results