We narrowed to 407 results for: pho

1. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

   Type

   Blog Post

   ...inactivation maximum of 590 nm light. Aurora and Phobos optogenetic tools - A collection of bi-stable anion... medicine, evolution of body plans, synthetic morphology, and even artificial intelligence," says Michael...

2. Antibodies 101: Affinity Reagents

   Type

   Blog Post

   ...and cells. They can be used either with or as fluorophores in a wide variety of colors.  To antibody or... the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction...

3. Career Coaching for Scientists: Why and Where Do I Find One?

   Type

   Blog Post

   ...costs low. Most will work with you remotely (by phone, Skype or Google Hangout) but in-person coaching...White Consulting frequently do sessions by Skype or phone. Samantha Sutton is another very experienced and...

4. Working with Nuclear Receptors

   Type

   Blog Post

   ...actions of the whole complex.  For example, the phosphorylation status of SRC-3 defines its association with...

5. A Guide to Designing a Scientific Poster: Content, Layout, and More

   Type

   Blog Post

   ...contact information, and potentially a photograph of yourself. A photo can be very useful in the event that...

6. Synthetic promoter AAVs for cell-type specific expression in retinal cells

   Type

   Blog Post

   ...types (ex: rods or cones) or cell classes (ex: photoreceptors which encompassed both rods and cones).  Targeted...also expressed. If Cre-mCherry expresses in photoreceptors and horizontal cells, and Cre-dependent-GFP...

7. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

   Type

   Blog Post

   ...frequent CDG type characterized by deficient phosphomannomutase (PMM) activity due to mutations in the PMM2...

8. Plasmids 101: FLEx Vectors

   Type

   Blog Post

   ... of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful? The power of the FLEx system...for clearer characterization of the location, morphology, and circuit mapping of a targeted neuronal population...

9. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

   Type

   Blog Post

   ...taking a snapshot of chromatin looping with the photo “developed” by analyzing DNA sequencing data. While... The high resolution and multiplexed chromatin ‘photos’ generated with CAPTURE 2.0 allow us to uncover...

10. Magnetic Control of Proteins: More than a Dream

    Type

    Blog Post

    ...responses of cryptochrome proteins or organic fluorophores (Karki et al., 2021; Lee et al., 2011; Matsuoka..., & Zoltowski, B. D. (2021). Cryptochromes: Photochemical and structural insight into magnetoreception...

11. Pooled Library Amplifications

    Type

    Blog Post

    ...Screening Rinehart Lab Iterative Synthetically Phosphorylated Isomers (iSPI) Libraries ~11,000–110,139 ...

12. Plasmids 101: Protein Expression

    Type

    Blog Post

    ...commonly used promoters include the phosphate and copper inducible PHO5 and CUP1 promoters respectively. ...

13. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type

    Blog Post

    ...mismatch repair assay? Or is PCR followed by gel electrophoresis and/or next-generation sequencing more appropriate...   Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines Transformed cell...

14. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    ...basic science research (neuronal tracing and morphologic studies), as well as translational applications...capsids are useful tools for neuronal tracing and morphology studies when used as part of a two part fluorescent...

15. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type

    Blog Post

    ...your samples and maintaining cellular and tissue morphology. Formaldehyde is the most commonly used cross-linker...reagents - cross-linkers better preserve sample morphology, while organic solvents can reveal otherwise ...

16. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    ...The kan resistance gene, NPTII, facilitates phosphorylation of kan, which inactivates it. The NPTII gene...

17. Course-Based Undergraduate Research Experiences (CUREs)

    Type

    Blog Post

    ...recently purified MDH to study the impact of phosphorylation on the enzyme’s structure, function, and interaction...

18. Antibodies 101: Producing Recombinant Antibodies

    Type

    Blog Post

    ...Using a spectrophotometer is among the faster methods of quantification. Many spectrophotometers have an...

19. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    ... This process can be avoided direct antibody fluorophore conjugates are used, but these are usually more...secondary nanobodies conjugated with the desired fluorophores. In figure 4B below, triple colocalization is...

20. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    ...including a lot of future Addgenies as shown in the photo below.  Kids and adults alike enjoyed some friendly...of your plans by email, gong, evite, posters, megaphone, or your company’s intranet. Don’t forget about...