We narrowed to 400 results for: pho;

1. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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   ...frequent CDG type characterized by deficient phosphomannomutase (PMM) activity due to mutations in the PMM2...

2. A Guide to Designing a Scientific Poster: Content, Layout, and More

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   ...contact information, and potentially a photograph of yourself. A photo can be very useful in the event that...

3. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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   ...types (ex: rods or cones) or cell classes (ex: photoreceptors which encompassed both rods and cones).  Targeted...also expressed. If Cre-mCherry expresses in photoreceptors and horizontal cells, and Cre-dependent-GFP...

4. Plasmids 101: FLEx Vectors

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   ... of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful? The power of the FLEx system...for clearer characterization of the location, morphology, and circuit mapping of a targeted neuronal population...

5. Plasmids 101: Protein Expression

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   ...commonly used promoters include the phosphate and copper inducible PHO5 and CUP1 promoters respectively. ...

6. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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   ...taking a snapshot of chromatin looping with the photo “developed” by analyzing DNA sequencing data. While... The high resolution and multiplexed chromatin ‘photos’ generated with CAPTURE 2.0 allow us to uncover...

7. Pooled Library Amplifications

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   ...Screening Rinehart Lab Iterative Synthetically Phosphorylated Isomers (iSPI) Libraries ~11,000–110,139 ...

8. Magnetic Control of Proteins: More than a Dream

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   ...responses of cryptochrome proteins or organic fluorophores (Karki et al., 2021; Lee et al., 2011; Matsuoka..., & Zoltowski, B. D. (2021). Cryptochromes: Photochemical and structural insight into magnetoreception...

9. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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   ...mismatch repair assay? Or is PCR followed by gel electrophoresis and/or next-generation sequencing more appropriate...   Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines Transformed cell...

10. Plasmids 101: Secondary Nanobody Toolbox

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    ... This process can be avoided direct antibody fluorophore conjugates are used, but these are usually more...secondary nanobodies conjugated with the desired fluorophores. In figure 4B below, triple colocalization is...

11. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    ...your samples and maintaining cellular and tissue morphology. Formaldehyde is the most commonly used cross-linker...reagents - cross-linkers better preserve sample morphology, while organic solvents can reveal otherwise ...

12. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    ...The kan resistance gene, NPTII, facilitates phosphorylation of kan, which inactivates it. The NPTII gene...

13. Antibodies 101: Producing Recombinant Antibodies

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    ...Using a spectrophotometer is among the faster methods of quantification. Many spectrophotometers have an...

14. Course-Based Undergraduate Research Experiences (CUREs)

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    ...recently purified MDH to study the impact of phosphorylation on the enzyme’s structure, function, and interaction...

15. AAVs CREATed for Gene Delivery to the CNS and PNS

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    ...basic science research (neuronal tracing and morphologic studies), as well as translational applications...capsids are useful tools for neuronal tracing and morphology studies when used as part of a two part fluorescent...

16. The Importance of a Fun Workplace: Company Culture at Addgene

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    ...including a lot of future Addgenies as shown in the photo below.  Kids and adults alike enjoyed some friendly...of your plans by email, gong, evite, posters, megaphone, or your company’s intranet. Don’t forget about...

17. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    ...this unique sequence of DNA and both identified lymphocyte antigen 6 complex (LY6A) as a receptor for PHP.B...discovered as an antigen upregulated on activated lymphocytes and is commonly used to enrich adult mouse hematopoietic...

18. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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    ...have diverse traits with good genetic mapping morphology and interesting behaviors. There is diversity...Chisholm) This cyanobacterium is the smallest photosynthetic cell in existence, but most abundant cell on...

19. Antibodies 101: Immunoprecipitation

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    ...sample lysates will also contain proteases and phosphatases that may degrade the target protein. To prevent...prevent this, include protease and phosphatase inhibitors in the lysis buffer, keep samples on ice, and ...

20. Twenty Years of Sharing: Expanding Accessibility

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    ...γ-Retrovirus Guide Protocols Agarose Gel Electrophoresis Bacterial Transformation How to Design a Primer... How to Design Primers for PCR Agarose Gel Electrophoresis Inoculating Liquid Bacterial Cultures Restriction...