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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 43 results for: pho;

Showing: 1 - 20 of 43 results

  1. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...generate the phosphosites with serine instead of phosphoserine (i.e. a “phosphorylatable” phosphosite library... serine phosphorylation ( Rinehart Human Serine Phosphopeptide Library ). The phosphorylation sites (“...Reagents Rinehart Lab Phosphoprotein Reagents Protein phosphorylation is one of the most abundant forms of...questions surrounding serine phosphorylation by adding this phosphorylated amino acid to the genetic code...or multiply phosphorylated proteins (Pirman et al., Nat Comm 2015). Improved phosphoprotein production ...(“phosphosites”) consist of a central phosphoserine site flanked by 15 amino acids on either side occurring... site-specific incorporation of phosphoserine in >36,000 phosphosites by tandem mass spectrometry (Barber...

  2. Immunology Research Plasmids and Resources

    Type
    Collection
    ...PLCG2 phospholipase C, gamma 2 (phosphatidylinositol-specific) - PPP3CA protein phosphatase 3 (formerly...IP1, IP2, IPD2, NEMO INPP5D inositol polyphosphate-5-phosphatase, 145kDa MGC104855, MGC142140, MGC142142...FLJ26625, JTK8 MALT1 mucosa associated lymphoid tissue lymphoma translocation gene 1 DKFZp434L132, MLT... PIK3CD phosphoinositide-3-kinase, catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase...and cytoplasmic domain, (semaphorin) 6D FLJ11598, KIAA1479 SEMA7A semaphorin 7A, GPI membrane anchor (...BMP1 bone morphogenetic protein 1 FLJ44432, PCOLC, PCP, TLD, pCP-2 BMP10 bone morphogenetic protein 10...MGC126783 BMP15 bone morphogenetic protein 15 GDF9B, ODG2, POF4 BMP2 bone morphogenetic protein 2 BMP2A BMP3...

  3. Genetic Code Expansion

    Type
    Collection
    ... Expansion Accessible Using Phosphoserine to Study Protein Phosphorylation Rinehart Lab reagents tRNA/... mutated, serB deleted for phosphoserine GCE. For expressing phosphoserine-containing proteins using genetic... pMAH2-CageCys leucyl-tRNA-synthetase E. coli photocaged cysteine Mammalian TAG Huiwang Ai 73544 pEvol-pAcFRS...91705 pSupAR-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers DiZPK, DiZSeK, or DiZHSeC Bacterial TAG P....91706 pCMV-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers DiZPK, DiZSeK, or DiZHSeC Mammalian TAG P.... PylT_FLAG-AbKRS-chIPYE chimeric PylRS Chimeric photo-crosslinking-lysine Mammalian TAG Simon Elsaesser...140022 pAS_4xMma PylT_FLAG-PcKRS PcKRS M. barkeri photocaged-lysine Mammalian TAG Simon Elsaesser 140023 pAS...

  4. Antibody Guide

    Type
    Collection
    ... curve with known amounts of protein. Fluorophores Fluorophores are light-sensitive molecules which emit...IF uses fluorophore color to differentiate between the targets of interest, if your fluorophore is bound...the fluorophores used is important. It is best to inversely pair the brightness of the fluorophore with...If a bright fluorophore is on an abundant target, it obscures fainter signals. Fluorophores can emit signals...storing your antibody: Antibodies conjugated to fluorophores should never be stored below 4 °C. Certain isotype...molecules are horseradish peroxidase (HRP) and fluorophores. Horseradish peroxidase (HRP) HRP performs a...specific wavelength. While more expensive than HRP, fluorophores are available in a range of colors activated...

  5. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Webb Phosphate FRET-based phosphate sensor (FLIPPi) A novel analytical method for in vivo phosphate tracking...Ca(2+) indicator with enhanced two-photon absorption. Neurophotonics. 2024 Apr;11(2):024207. doi: 10.1117...29;7. pii: 32638. Kai Johnsson Phosphate Scaffold for inorganic phosphate biosensor Development of a Reagentless.... Eric Schreiter Calcium AAV expression of photoconvertible fluorescent protein-based calcium integrator...calcium sensor with exceptional brightness, photostability, and multiplexing capabilities. bioRxiv. 2025... jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths. Nat Methods...localized zinc sensor ZIBG2 Genetically Encoded, Photostable Indicators to Image Dynamic Zn(2+) Secretion ...

  6. Ras Pathway

    Type
    Collection
    ... Protein phosphatase 1 catalytic subunit alpha PREX2 Phosphatidylinositol-3,4,5-trisphosphate-dependent...receptor PDPK1 3-phosphoinositide dependent protein kinase 1 PEBP1 Phosphatidylethanolamine binding protein...PIK3R5 PIK3R6 Also known as PI3K; Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and regulatory...state, regulated by the addition and removal of a phosphate group. Under normal physiological conditions GTPases...thymoma viral oncogene homolog 3 ALK Anaplastic lymphoma receptor tyrosine kinase ARHGAP35 Rho GTPase activating...DUSP2 DUSP3 DUSP4 DUSP5 DUSP6 Dual specificity phosphatase E2F E2F1 E2F2 E2F3 E2F transcription factor ECT2...G1, G2, G3: non-catalytic subunit gamma PTEN Phosphatase and tensin homolog RAC RAC1 RAC2 RAC3 Ras-related...

  7. Optogenetics AAV Preps

    Type
    Collection
    ... Dlx EF1a/nEF Synapsin E2 regulatory element Fluorophore GFP Red-wavelength fluorescent protein Yellow-wavelength...pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE CamKII ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Deisseroth...pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Deisseroth...pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE EF1a ChRmine (high-photocurrent, red-shifted) mScarlet Cre dependent 1, 5 Deisseroth... 6m-p2A-ChRmine-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) GCaMP6m Constitutive 8 Deisseroth... pAAV-nEF-ChRmine-mScarlet nEF ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Deisseroth...pAAV-nEF-Con/Fon-ChRmine-oScarlet nEF ChRmine (high-photocurrent, red-shifted) oScarlet Cre and Flp dependent...

  8. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ... multicolor imaging. Photo-activatable iRFPs can be ‘turned on’ by non-phototoxic far-red light and are...690/717 (after photoactivation) 3.2 pPAiRFP1-N1 PAiRFP2 692/719 (after photoactivation) 3.0 pPAiRFP2-N1... imaging using far red, near-infrared, and photoactivatable fluorescent proteins. Plasmid...imaging are derived from bacterial phytochrome photoreceptors (BphPs). Spectrally distinct permanently fluorescent...The following far red , near-infrared , and photoactivatable fluorescent empty backbone plasmids are ideal...piRFP720-N1 iSplit 690/713 5.3 pPAS-E and pK-GAFm Photoactivatable Protein Excitation/Emission Brightness Find...

  9. Bacterial Expression Systems

    Type
    Collection
    ...collection of FPs, including photoactivatable, photoconvertible, and photoswitchable proteins, organized by ...when a donor fluorophore is excited, it can transfer its energy to an acceptor fluorophore if they are ...Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent Proteins Fluorescent Protein Timers...fluorescence both from the donor and the acceptor fluorophore by only exciting the donor. In BiFC, proteins...Inducible Promoters Repressible Promoters Synthetic Photobiology: Optogenetics for E. coli Return to top Reporter...

  10. Recombinases AAV Preps

    Type
    Collection
    ...these molecular tools. Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI....AAV.TBG.PI.Cre.rBGe TBG none 8 Wilson Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA...Syn none 5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1(S)-FlpO-bGHpA...Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype(s) PI 140135 pAAV-EF1a-iCreV EF1a none...none 1, PHPeB Zeng VCre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 55638 pAAV-EF1a-vCre EF1a ...

  11. Luciferase Plasmid Collection

    Type
    Collection
    ... of photons. Its robust signal, sensitivity, wide dynamic range, and resistance to photobleaching have...Firefly Creation of N-terminal Firefly fusions in Drosopholia Michael Rosbash 128046 pGL3basic luciferase and... from the luciferase reaction to an acceptor fluorophore can provide more flexibility in emission colors...BRET energy donor and HaloTag labeled with a fluorophore as the acceptor. LumiFluors: EGFP-NanoLuc ( GpNLuc... sensor created by fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Luminopsins...

  12. Zhang Lab CRISPR Page

    Type
    Collection
    ...to enable the rapid ligation of annealed and phosphorylated oligos designed based on the target site sequence...

  13. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Near-Infrared Long Stokes Shift Photoactivatable Photoconvertible Photoswitchable Timers Blue/UV Protein Excitation...LSSmKate2-C1 - Mammalian Expression Jump to Top Photoactivatable (e.g. off to on) Excitation and Emission wavelengths...HisB-PAiRFP2 - Bacterial Expression Jump to Top Photoconvertible (e.g. green to red) Protein Excitation (nm...pBAD-PSmOrange2 - Bacterial Expression Jump to Top Photoswitchable (e.g. off to on to off) Protein Excitation ...

  14. mTOR Pathway

    Type
    Collection
    ...PIK3R2 PIK3R3 PIK3R4 PIK3R5 PIK3R6 Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and regulatory...AKT1S1; AKT1 substrate 1 (proline rich) PTEN Phosphatase and tensin homolog Rag RRAGA RRAGB RRAGC RRAGD...Protor Also known as PRR5; proline rich 5 PTEN Phosphatase and tensin homolog Rictor RPTOR independent companion...

  15. p53 Pathway

    Type
    Collection
    ...deprivation. p53 is activated through both phosphorylation and acetylation, which disrupt its interaction...ligase MDMX p53 regulator; also known as MDM4 Noxa Phorbol-12-myristate-13-acetate-induced protein 1; also... 1 (RCHY1); E3 ubiquitin protein ligase PTEN Phosphatase and tensin homolog PUMA BCL2 binding component...

  16. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...2.1 may be omitted. CRISPR Cloning Anneal and phosphorylate oligos. Resuspend oligos at a concentration ...Polynucleotide Kinase (PNK) (10,000 U/ml). NOTE: Phosphorylated oligos may be ordered instead. For this approach...

  17. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...followed by a quick ligation of annealed and phosphorylated oligonucleotides containing the guide target...Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs...

  18. Antibody Production

    Type
    Collection
    ...are initially titered by spectrophotometry using a NanoDrop spectrophotometer. If needed, the sample is...centrifugal columns. The final formulation buffer is phosphate-buffered saline containing 1 mM sodium azide. ...

  19. Zebrafish Plasmid Collection

    Type
    Collection
    ...transposons, Cre-mediated site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9...of gene function in zebrafish - Julian Lewis Lab PhOTO Zebrafish: A Transgenic Resource for In Vivo Lineage...consisting of plasmids with four promoters, six fluorophores, and empty vectors designed to streamline the...

  20. Brain Initiative Collection

    Type
    Collection
    ...Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal...parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades...parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades...
Showing: 1 - 20 of 43 results