We narrowed to 290 results for: poly

... and biological. Even the most high-fidelity polymerases can make mistakes and lead to the accumulation...

... La to protect the end of the pegRNA. Adding 3′ polyU tracts to the end of pegRNAs (but not epegRNAs) ...

...antibodies. You’ll want to set up your blot with a polyvinylidene difluoride (PVDF) membrane, which is sturdier...

...technology park, it enjoys preferential governmental policies for a high-tech development zone so many science...

...-aggressives, who are usually antagonistic and impolite, Complainers, who spend most of their time moaning...

...want to take over? It is the moderator's job to politely but firmly get them to stop talking and redirect...

...catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct is designed to activate...

...the surface of the protein where the nascent polypeptide emerges from the exit channel.   He then said...

...HaloTag, SpyTag). The Foerstemann lab developed a polymerase chain reaction (PCR)-based system that allows...

..., Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the gene. This ...

...transporting proteins in yeast by using murine polyomavirus virus-like particles (MPyV VLPs ). This artificial...

...endogenous CRBN E3 ligase complex which will polyubiquitinate the fusion protein. This will target the protein...

...rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of 40% PEG solution...

... (cap) genes. AAV replication uses the host’s polymerase, but requires Rep proteins to process a double-stranded...

...Chemical transfection   Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines...

...intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts...

...have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the Broad’s Genomic...

...initiating the whole process – such as the viral DNA polymerase – it makes sense that vectorization strategies...

...resources on the Addgene blog Read more about polymerase chain reaction Browse our blog posts related ...

...production: Gag (structural precursor protein), Pol (polymerase), Tat (viral transactivator for transcriptional...