We narrowed to 291 results for: poly

1. Plasmids 101: Degron Tags

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   ...endogenous CRBN E3 ligase complex which will polyubiquitinate the fusion protein. This will target the protein...

2. Truncated gRNAs for Regulating Gene Expression

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   ..., Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the gene. This ...

3. Hot Plasmids and Viral Preps - May 2021

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   ...transporting proteins in yeast by using murine polyomavirus virus-like particles (MPyV VLPs ). This artificial...

4. Tips for a 1st time AAV user (by a Rookie AAV user)

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   ... (cap) genes. AAV replication uses the host’s polymerase, but requires Rep proteins to process a double-stranded...

5. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   ...catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct is designed to activate...

6. 3D Printing Meets CRISPR Cas9

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   ...the surface of the protein where the nascent polypeptide emerges from the exit channel.   He then said...

7. Fluorescent Tagging of Endogenous Genes with SapTrap

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   ...HaloTag, SpyTag). The Foerstemann lab developed a polymerase chain reaction (PCR)-based system that allows...

8. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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   ...Chemical transfection   Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines...

9. An Introduction to Adenovirus

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   ...initiating the whole process – such as the viral DNA polymerase – it makes sense that vectorization strategies...

10. Uncovering Ancestral Bias in CRISPR Research

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    ...have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the Broad’s Genomic...

11. Droplet Digital PCR for AAV Quantitation

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    ...resources on the Addgene blog Read more about polymerase chain reaction Browse our blog posts related ...

12. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ...intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts...

13. Lentiviral Vector Uses and Overview

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    ...production: Gag (structural precursor protein), Pol (polymerase), Tat (viral transactivator for transcriptional...

14. CRISPR 101: Any Base Transversion Editors

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    ...they co-expressed Polη, a translesion synthesis polymerase known to preferentially incorporate A bases across...

15. Antibodies 101: The Four ELISAs and When to Use Them

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    ... Selecting the Right Antibody Antibodies 101: Polyclonal Antibodies Resources on addgene.org The Addgene...

16. Plasmids 101: Stringent Regulation of Replication

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    ... from the ori. In the elongation phase, a DNA polymerase synthesizes a new DNA strand at each replication...

17. Plasmids 101: Broad Host Range Plasmids

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    ...structural elements, oris interact with various RNA polymerases and proteins from bacterial hosts. In a plasmid...

18. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    ... the cells could be batch-sorted to isolate a polyclonal cell line with strong gas vesicle expression ...

19. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    ...engaging target DNA. The reverse transcriptase polymerizes DNA onto the target strand based on the sequence...

20. Viral Vectors 101: Pseudotyping

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    ...which contains a promoter, the envelope gene, and a polyA tail.   Figure 1: Lentiviral production uses...