We narrowed to 361 results for: reporter

1. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

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   Blog Post

   ...correlative biochemical characteristics and ectopic reporter assays. We have been interested in exploring the...

2. pSiM24: Simplifying Plant Genetic Engineering

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   Blog Post

   ... corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple transient and transgenic ...

3. Reflections and Looking Ahead

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   ...viral vector serotypes, or hundreds of Data Hub reports detailing antibody experimental results. We also...

4. New Viral Vectors - Fall 2024

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   Blog Post

   ...check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the link...

5. Cellosaurus: A Cell Line Information Database

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   Blog Post

   .... For instance, recently Cellosaurus received a report that HT-55, which was described as a colon cancer...

6. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

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   Blog Post

   ...also captured as metadata.   Screen results are reported as the original quantitative gene-level scores...

7. Custom CRISPR Screens & the Green Listed Software

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   ... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed...therapeutic targets in acute myeloid leukemia." Cell reports 17.4 (2016): 1193-1205. PubMed PMID: 27760321. ...

8. Sequencing Options for CRISPR Genotyping

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   ... unbiased methods, high background BLESS Not reported Cell-based (in vitro) Can be used on tissue from...senstive to time since cell fixing HTGTS Not reported Cell-based Identifies translocations Limited by...

9. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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   Blog Post

   ... targeting astroglia. It has additionally been reported to have tropism for lung, liver, vascular endothelial...Use Addgene’s AAV Data Hub to find experimental reports on commonly used vector cargo designs and administration...

10. Plasmids 101: Using Transposons in the Lab

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    ...piggyBac plasmids    Tol2  Tol2 was the first reported active DNA transposon in vertebrates. It was discovered...mutations. In these screens, the transposons encode reporter genes, mutagenic cassettes, or barcodes. When ...

11. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    Blog Post

    ...gene (e.g., channelrhodopsin-2, ChR2) fused to a reporter gene (e.g., mCherry) to easily detect opsin-expressing...recombinase in mouse primary afferents." Scientific reports 8.1 (2018): 7321. PubMed PMID: 29743652. PubMed...

12. New Viral Vectors - Winter 2025

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    Blog Post

    ...help, with over 100 user-submitted viral vector reports. And don't forget to use the link below to keep...

13. New and Upcoming Viral Vectors - Spring 2019

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    Blog Post

    ...with implanted electrodes. The Archon1 voltage reporter allows a way to monitor neuronal electrical activity...

14. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    Blog Post

    ...following sucrose treatment. Moreover, several reports have shown that cells treated with sucrose or other...

15. Hot Plasmids and Viral Preps - July 2021

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    Blog Post

    ...straightforward workflow for doing gene targeting, reporter generation, controllable gene knockouts, and inducible...

16. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    Blog Post

    ...’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins such...

17. Plasmids 101: What is a plasmid?

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    Blog Post

    ...expression plasmids, gene knock-down plasmids, reporter plasmids, viral plasmids, and genome engineering...

18. Summer SciComm Series: Master of SciComm

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    Blog Post

    ...itself. Several years of exclusively writing lab reports and empirical essays had also stirred some of my...

19. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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    Blog Post

    ... rather than to rely on nuclei extraction. They report that this change offers a significant improvement...

20. The Strength of Story Telling

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    Blog Post

    ...taking beautiful photographs of their studies, reporting on amazing women in science, or asking someone...