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We narrowed to 838 results for: tat

Showing: 201 - 220 of 838 results

  1. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    .... For example, mutations in the RBE site reduce binding with Rep proteins and mutations in the trs site...and C-C’ palindromic sequences determines the orientation of the ITR, which can either be “flip” or “flop...Figure 2: AAV2 wild type ITR in flip and flop orientation. The ITR contains three palindromic sequences...important for viral genome replication, these mutations can affect transduction efficiency and subsequent...are prone to replication errors and disruptive mutations and truncations that can make vector propagation...I maintain ITR integrity and prevent unwanted mutations?  During propagation, mutants rapidly replicate...result in a high percentage of plasmid DNA with mutated ITR sequences. Using recombinant-deficient bacterial...

  2. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...your particular field of research.   Citations tools DOI Citation Formatter Just enter the DOI of a paper...generating citations for a few papers at a time. Mendeley Mendeley works much like other popular citation managers...phrases commonly used in research papers or dissertations. Phrases are categorized either based on the... systems.  Zotero  Zotero is also another free citation manager that lets you organize and cite papers...chart highlights the differences between these two citation managers. If you want more tips on finding and...podcast episode 2 "Essential Grad School Resources: Citation Managers, Programming, & Productivity": This podcast...podcast talks about Mendeley, Zotero, and other citation managers and productivity tools for grad school...

  3. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...their excitation and emission spectra, but a good rule of thumb is that both the peak excitation wavelengths...substantial overlap between the excitation and emission filters and the excitation and emission spectra of the...filter set but the excitation filter excites more of the peak of the TagRFP excitation and the emission ... requires multiple fluorescent proteins whose excitation and emission spectra differ sufficiently for ...microscope optics, you will want to compare the excitation and emission spectra of your protein with the...mCherry spectrum is shown in the lighter colors; excitation spectra are blue and emission spectra are red...

  4. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...variants or mutations have been reported in people, we can use this kit to knock in those mutations. Once that's... you can either knock out a gene or knock in a mutation. Addgene: How do you use this kit to target a ...adipocytes or neurons, for instance – to see how those mutations influence the disease process. In the Cell Stem... large number of individuals and find several mutations in the same gene or in multiple genes, all of ...which seem to contribute to a particular disease state. You could use the kit to insert all of those variants...do an apples-to-apples comparison of all those mutations. If you make induced pluripotent stem (iPS) cells...to compare those lines, they may all have the mutations but they will also have different genetic backgrounds...

  5. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...FRET donor excitation wavelengths overlap with optogenetic initiation wavelengths, excitation of the optogenetic... to lab. Promega supports the Addgene mission statement: Accelerate research and discovery by improving...reporters produced bright signals without exogenous excitation and with OgNLuc being the brightest since it ...co-imaged with GFP using the same wavelength for excitation. The result was the cyan-excitable orange fluorescent...molecularly evolved from mNeptune2 through 33 mutations and 2 deletions. CyOFP1 has a higher quantum yield...fluorescent proteins (FPs) can be problematic because excitation light can cause phototoxicity, autofluorescence...sensor with the following characteristics: No photoexcitation High Ion specificity High sensitivity Easy ...

  6. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...will spend the next 6 years. Know yourself I rotated in 3 labs before choosing a graduate school lab...but the labs were profoundly different. My first rotation was with a mid-sized lab with an experienced mentor...biting my tongue and gratefully moved on. My second rotation was in a big lab with a famous PI who I was only...collaborative learning style at all. Finally I rotated in a small lab with a new PI. I realized that as...well. What do they have to say about this lab’s reputation? If you are choosing a training lab, I strongly...strongly suggest you complete a work rotation in the lab if at all possible. If not, see if you can spend ...is workplace bullying? Threats to professional status – including belittling opinions, public professional...

  7. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...heterozygous mutations, it will clearly be much more challenging for homozygous mutations. Moreover, introducing...towards CRISPR germline editing of disease-causing mutations. But how close are we really, and what new questions...interested in correcting an autosomal dominant mutation in MYBPC3 that leads to hypertrophic cardiomyopathy...designed a repair template with two synonymous mutations to distinguish it from the wild-type allele, they...controls.) 27.6% of embryos carried indels at the mutation site, indicating that repair proceeded through...title the paper “Correction of a pathogenic gene mutation..” rather than using term ‘editing.’ While repair...cardiomyopathy and other genetic disorders, pre-implantation genetic diagnosis (PGD) screens embryos to find...

  8. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...Creatine Deficiencies: Compensating for SLC6A8 mutations in Creatine Transporter Deficiency Creatine Transporter...There is no cure for CTD, which arises due to mutations in the SLC6A8 gene, also known as the creatine...barrier using creatine transporters. However, mutations in the transporter SLC6A8 impede this transport...thus informing potential for success in a patient-mutation bearing knock-in mouse model for CTD. Congratulations...euploid iPSCs. “The BeHEARD Addgene reagents will facilitate our aims to correct X chromosome gene dosage ...Congratulations Siddharth Prakash! Terry Hébert: How GNB1 mutations affect signaling pathways G protein-coupled receptors... work together on the cell membrane. Germline mutations in one of these parts, the GNB1 gene, which codes...

  9. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...cells with a membrane possess an electrical state. This state, known as the resting potential (Vmem), can... junction proteins cx43 GJB1 GJB2 Bioelectric states can alter cell types and patterning There is mounting... or cancerous it is (Levin et al., 2017). These states can even be necessary and sufficient for certain...visualize or perturb these slower-changing bioelectric states in living cells. Since bioelectrical signals are...indicators (GEVI) are ideal tools to track bioelectric states (Matthew et al., 2018). These allow for not only...only recording but also modulating bioelectric states in vivo. For example, the Levin lab from Tufts University...domain of a Ciona intestinalis voltage sensor and a mutated version of the pHLuorin fluorescent protein. This...

  10. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...strand via mutations N497A, R661A, Q695A, and Q926A (SpCas9-HF1 contains all four mutations). Although...Additional work revealed that mutations in the mobile Rec3 domain facilitate this stall. With this new found... authors went on to combine the best of these mutations and tested the combination mutants for their on-target...mutants are not toxic to HEK cells and that similar mutations can improve the specificity of Cas9 derived from...This means you can likely apply similar rational mutations to your Cas9 homolog of choice and get improved...Although the precise mutations were different than those found in eSpCas9, the effect on specificity was similar...therapeutic settings. Will combining the different mutations found in each of these great tools further enhance...

  11. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...Bimolecular Fluorescence Complementation (BiFC). It can provide a qualitative or quantitative readout of the interaction...protein fragments for bimolecular fluorescence complementation analysis under physiological conditions. Shyu...VC155 An improved bimolecular fluorescence complementation assay with a high signal-to-noise ratio. Kodama...fluorescent reporter with rapid and reversible complementation. Tebo AG, Gautier A. Nat Commun. 2019 Arnaud...the MoBiFC modular bimolecular fluorescence complementation toolkit from Benjamin Field’s lab. Special ...maturation, splitFAST displays rapid and reversible complementation. TagBiFC (split HaloTag) provides maximum flexibility...

  12. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...caused by a mutation in a single gene, it can potentially be treated by correcting the mutation or replacing...bubble boy syndrome. This disease is caused by mutations in the common cytokine receptor gamma chain (c... so, their T-cells were grown in vitro, their mutations corrected, and the T-cells were transferred back...T-cells outside the body and thereby correct the mutations using retroviral vectors. However, many diseases...improved specificity. AAV vectors are able to facilitate and enhance DNA repair via homology directed ...especially amenable to correcting disease-associated mutations. AAV vectors maintain persistent transgene expression...congenital amurosis (LCA). This disease is caused by mutations in the gene RPE65. It is characterized by childhood...

  13. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...reason to give presentations - networking! People get to know you through your presentations and will be ... grant (e.g. an R01 from the NIH, in the United States) Strong letters of reference that attest to your... they've made enough progress since the last presentation. Yet, PFs need to tell and sell their stories...recent relevant studies in the literature. Plus, presentations benefit from practice. If you simply recycle...recycle slides from the last time you gave a presentation to a specific group and tack on a few new pieces ...attention to how the audience responds to your presentations, you will become a more confident and skilled...The group leader had attended my department presentations and could speak knowledgeably about my research...

  14. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...cancer-related complications each year. Despite these grim statistics, new strategies for attacking cancer are yielding...pedal of a vehicle, respectively. In the case of a mutation that turns a proto-oncogene permanently “on”, ...accelerator of a vehicle being stuck down. Similarly, a mutation that turns a tumor suppressor “off” is analogous...From proteins to pathways - Ras as a model When mutations do arise and cancer ensues, traditional chemotherapies...proteins are special because they are known to be mutated in as many as 30% of all cancers, especially those...pancreas and colon. Despite the prevalence of Ras mutations in a variety of cancers, Ras proteins have stumped...synthesizing compounds to bind specifically to their mutated residues. For Ras researchers, one of the most ...

  15. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...can vary depending on factors like socioeconomic status and geographic location. For example, not all social...Otherwise, you’re going to drop your project like a hot potato or burn yourself out trying to slog through unappealing...communicate and I wholeheartedly encourage experimentation. You never know what's going to work. After...program? Comparing your desired outcome to the limitations and potential of your preferred platform before... your audience. Digital or print distribution.  Static. Can be difficult to convey an entire story or ...omitting details. Digital or print distribution.  Static. Often requires both artistic and scientific training...for children” or may need text for audience interpretation. Infographics Low Combines both words and...

  16. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...for plant adaptation and speciation. Their phenotypes are diverse and they inhabit all habitats - even 50...this wonder of the organism.   It’s not an overstatement to say that CRISPR/Cas9 editing is one of the...which these methods are presently unavailable.” As stated by one of the NSF program directors, this program...community is no small task. The EDGE program solicitation requires funded labs to rapidly disseminate ...possible nonetheless - the first example was of a pigmentation gene.   Genus Microtus (Zoe Donaldson) Voles...methods were available to create targeted gene mutations in anoles lizards or in any reptiles at all. But...was able to make an albino lizard with targeted mutations. Now what? What would you study first? They are...

  17. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...Relevant mutations/deletions" box to describe any mutations within your insert using standard mutation notation...consequences of that change. For example, "Mutated Aspartate 123 to Lysine (D123K), lowers biosensor affinity...pre-publication plasmids" and set a desired distribution status. You can submit "Published plasmids": This option...of the deposit. Select your desired distribution status: Hold for publication: we will initiate our ...fusion proteins contained in the plasmid, any mutations, and the intended purpose of the plasmid. Once...sequence the full plasmid and confirm features you annotate on this page. Figure 7: Enter information about...

  18. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ..., the monomeric state of a probe leads to less interference with the oligomeric state of a fusion partner....8 33 State-of-the-art NIR FPs, which combine high cellular brightness and monomeric state, are highlighted...there is a Table that summarizes the properties of state-of-the-art probes, many of which you can find at... heme metabolism (Shemetov et al, 2017). Modern state-of-the-art NIR FPs can be used similar to GFP-like... by an apoprotein and protein expression level. State-of-the-art FPs (listed in Table 1) were optimized... Molecular brightness vs. iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa...that this post helps you in getting to know the state-of-the-art NIR fluorescent probes and in choosing...

  19. Evolution of Lab Techniques

    Type
    Blog Post
    ...particular gene mutation, southern blotting can be used to identify the presence of that mutated gene. In southern...the presence of the mutated β globin gene that causes sickle cell anemia. The mutation happens to destroy...from their evolution. Southern blotting and quantitative real-time PCR Southern blotting, named for its...the probe (for example, has the sequence of the mutated gene the researcher is searching for), then the...southern blotting being used to identify a gene mutation is the genetic test used for sickle cell anemia...analytical digests. A researcher can test the orientation of an insert in only a few hours at low cost ...Frank Speleman, and Jo Vandesompele. "Real-time quantitative PCR as an alternative to Southern blot or fluorescence...

  20. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...low resolution or were very time-intensive, necessitating the development of a new technique. In a structure...fluorescent protein coding sequences in opposite orientations. Cre-mediated excision removes one segment, ...neurons, the system also suffered from a number of limitations. First, the imaging of Brainbow mouse tissue ...Brainbow-1 and Brainbow-2 each contained a “default” state; for example, Brainbow-1.0 expresses RFP when the...construct has not undergone recombination. This default state was disproportionately expressed by a majority of...Brainbow-3.0, with the goal of overcoming the limitations listed above. First, they screened a variety ...mKate2 as the fluorophores; mOrange2 is the default state. In contrast, Brainbow-3.1 and -3.2 do not display...
Showing: 201 - 220 of 838 results