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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 254 results for: tre promoter

Showing: 201 - 220 of 254 results

  1. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...an enhanced pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  Figure 1...dynamics of the motor protein kinesin-1, the Twelvetrees Lab took advantage of the self-labeling tags ...Turner, E.D., Abdelhamid, M.A.S., Craggs, T.D., & Twelvetrees A.E. (2024) Kinesin-1 is highly flexible and ...

  2. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...shown in tables 1 and 2, with a cell-type specific promoter or enhancer now allows for cell-type-restricted...special CREATE cap plasmid, loxP sites flank the downstream polyA sequence. When viruses produced from this...site of the injection. IV injection into the bloodstream allows for dispersion of the virus but it has...applications (mechanistic studies and gene therapy treatments for neurological diseases). The Gradinaru Lab...

  3. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...regulatory regions in the genome, including enhancers, promoters, insulators, and more. I then identified valid...and stop. For instance (chr3, 1, 10) indicates a stretch of DNA at the very beginning of the third chromosome... Let’s now examine how we can locate powerful stretches of DNA known as enhancers with the help of genome...light on dark matter Precise knowledge of which stretches of the genome are pillars for stem cells, or metastasized...

  4. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...continuous outflow of phage, increasing stringency and promoting survival of only the broadest-targeting variants..., also has a strict editing window about 15 nt upstream of the PAM. Even if there’s a PAM near your insertion...

  5. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...a requirement. Your manager: Unlike academia, promotions in industry are based on people’s ability to ...afford to spend too much time working on something extremely risky or exploratory. Getting a job afterwards...

  7. Tips for arabidopsis transformation

    Type
    Blog Post
    ...are binary gateway vectors and can be used for promoter swapping and generating N and C terminally tagged...healthy plants: Your plants should show no signs of stress, such as anthocyanin production, or disease, such...When your T1 is on soil and has recovered from the stress of selection you can clip a leaf and extract genomic...

  8. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...toxicity. AAV vectors can be equipped with strong promoters for high and neuron-specific transgene expression...AAV2-retro vectors with a variety of transgene/promoter combinations. Check out our retrograde AAV blog...forming a neuronal loop which is activated and strengthened during memory formation and memory retrieval..., causes neurotoxicity, and is lethal if left untreated (for review see Dietzschold et al., 2008). Herpes...PubMed Central PMCID: PMC3275419. Maday, S., Twelvetrees, A.E., Moughamian, A.J., and Holzbaur, E.L.F....Roberts, T. F., Hisey, E., Tanaka, M., Kearney, M., Chattree, G., Yang, C. F., Shah, N.M., and Mooney, R. (...

  9. Zebrafish Plasmid Collection

    Type
    Collection
    ...plasmids with four promoters, six fluorophores, and empty vectors designed to streamline the production of...Zebrafish Society (IZFS) - An organization that promotes and advocates for zebrafish research in the international...

  10. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...skills guide: managing a team. You’ve just been promoted, congratulations! You’re now a manager with your...motivates you!), know their unique skills and strengths (you will likely have reports with different levels...

  11. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...while also broadening access to experiences that promote professional success (many of my students include...1977; Ma et al., 2020). Many have interpreted this trend as evidence that the best teachers and mentors pass...

  12. Lab to Office Culture Shock

    Type
    Blog Post
    ...be these skills that get you your next job or promotion. Also, don’t wait until you’re out of the lab ...question today, because I sometimes wish for a stretch of time that doesn’t involve critical thinking....

  14. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ... difference between them lies in the choice of promoter for ωRNA (reRNA) expression (see Figure 1). Both... PAM sites. For Cas9, the PAM is located just downstream (3′ end) of the target sequence, while for Cas12a...Cas12a and TnpB, the motif is found upstream (5′ end) of the target site. Additionally, the TAM changes... region binds to the DNA sequence immediately downstream of the TAM. This RNA-DNA hybrid formation recruits...cleaves the DNA at a position 15–21 base pairs downstream of the TAM, creating staggered double-strand ...target gene. Choose a 20-nt sequence immediately downstream of the TAM for targeting. While plant-specific...

  15. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...enough to prevent confluency but high enough to promote cell growth. Overgrown cultures stimulate quiescence... yeast, fungi, or bacteria, mycoplasma can be extremely hard to detect with levels reaching 108 cells ...Daily medium exchanges help alleviate cellular stresses associated with pH changes and resource consumption...particles you harvest. Studies have demonstrated that extreme changes in osmotic pressure and pH can destabilize...labs try to use freshly collected lentivirus for downstream applications. While this ensures that the highest...23179822. 5. Segura, M.M., Kamen, A., Garnier, A. “Downstream processing of oncoretroviral and lentiviral gene...

  16. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...beneficial for many systems. How the EDGE Program promotes scientific sharing The EDGE Program is designed...to study life’s innovations from anywhere in the tree of life.” Accepted proposals were required to present... arose in different branches of the evolutionary tree at least 6-7 times and on at least 2 separate continents...especially cardenolides which have been used to treat heart disease. Caterpillars that eat milkweed generate...Joshua Rosenthal) The Hawaiian bobtail squid has an extreme number of nerve cells and an unusually high level...

  17. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...interest. A low primer-to-template ratio is used to promote annealing of one primer to each template. The PCR...restriction site, but nick opposite strands of DNA. Treating the plasmid with ExoIII and ExoI then fully degrades...by Wrenbeck et al but it’s likely that longer stretches of sequence could be used. However, a practical...assessing protein function on a massive scale." Trends in Biotechnology 29.9 (2011): 435-42. PubMed PMID...

  18. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ..., and plants. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID ...spacer sequence and contain a PAM sequence just downstream of the sequence to be cleaved. This non-specific...

  19. CRISPR Guide

    Type
    Collection
    ...are often used to change an amino acid, turn a promoter on/off, or add small protein tags. Precise Modifications... editors include several fused components that promote the intended repair pathway or inhibit unwanted... converting cytidine to uridine. However, they promote the base excision repair pathway, which fully removes...improved PE2/PE3 to inhibit mismatch repair to promote desired edit epegRNA - addition of a structured...while activation and repression libraries target promoter or enhancer regions. As each library is unique...transcriptional repressors or activators and targeting promoter regions. You might sometimes see these referred...the methylation state of cytosines in a gene’s promoter or by inducing histone acetylation or demethylation...

  20. CRISPR Plasmids - gRNAs

    Type
    Collection
    ... or repression experiments use targets within promoters. Do you have gRNAs you'd like to add to the Addgene...wild-type SpCas9 must be used with targets that are upstream of a 5' NGG 3' PAM sequence. Which CRISPR application...
Showing: 201 - 220 of 254 results