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Showing: 221 - 240 of 272 results

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Fluorescent Proteins 101: Aptamer Fluorophores

Type

Blog Post

...stable in cells. The new aptamer also has a higher melting temperature than its Spinach ancestors, and has...
Mesothelioma - Causes, Symptoms, and Treatment

Type

Blog Post

... mean that most mesothelioma patients have an extremely poor prognosis. Together, the cause, long latency...
Using Video to Share Your Science: We Share Science

Type

Blog Post

... “Singing” Fish Rely on Circadian Rhythm and Melatonin for the Timing of Nocturnal Courtship Vocalization...
Getting the Most from Your Lentiviral Transduction

Type

Blog Post

... yeast, fungi, or bacteria, mycoplasma can be extremely hard to detect with levels reaching 108 cells ...
Antibodies 101: Normalization and Loading Controls for Western Blots

Type

Blog Post

...detection range, band intensity measurements become extremely susceptible to error, since tiny changes in intensity...
Hot Plasmids: Summer 2024

Type

Blog Post

...Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., … & Weissman, J. S. (2021). Genome-wide...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

.... In fact, for several FRET biosensors (yellow cameleon 3.60, EPAC, and Galphai), the C-terminus and N-terminus...
Transferable Skills Guide: Conflict Resolution

Type

Blog Post

...other just for the sake of disagreeing. It can be extremely toxic in a lab or work environment if left unchecked...
Hot Biosensors 2022: Year-End Roundup

Type

Blog Post

...acssensors.1c02201. LiLac sensor for lactate Stop and smell the flowers - or rather, the lactate! The latest...
CRISPR 101: Validating Your Genome Edit

Type

Blog Post

...This post was originally written by Melina Fan and updated Nov 3, 2022 by Susanna Stroik. You’ve designed...
Antibodies 101: Introduction to Gating in Flow Cytometry

Type

Blog Post

...of my previous experiments where I harvested a melanoma tumor from a C57BL/6 mouse and analyzed the tumor-infiltrating...
An Integrin Antibody Toolkit from IPI

Type

Blog Post

...This pocket-binding property in an antibody is extremely rare. While many function-blocking integrin antibodies...
Plasmids 101: Biotinylation

Type

Blog Post

...molecule of biotin. Biotin and avidin have an extremely strong affinity for one another. Actually the ...
Multiple Plasmids at a Low Price: Kits from Addgene

Type

Blog Post

... Huang, Shijie, Xuechen Zhu, and Charles E. Melançon III. "Detection and Quantification of Ribosome...
Site Directed Mutagenesis by PCR

Type

Blog Post

...introduced anywhere in the plasmid (albeit at extremely low frequency), and these could interfere with...
Evolution of Lab Techniques

Type

Blog Post

...and behavior. References 1. Southern, Edwin Mellor. "Detection of specific sequences among DNA fragments...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Type

Blog Post

...CRISPR may be the way to accomplish this tough job! Kamel Khalili’s lab at Temple University has demonstrated...
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Type

Blog Post

...recommends an overlap of 15-40 bp, with a primer melting temperature greater than 48℃. Both Snapgene and...
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Type

Blog Post

...labeling for neural circuit analysis in Drosophila melanogaster. Hadjieconomou D, Rotkopf S, Alexandre C, Bell...
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

Type

Blog Post

...Miura et al. (2015) developed the ivTRT method to ameliorate this problem. Briefly, you design your donor ...

Showing: 221 - 240 of 272 results

Results per page:

Fluorescent Proteins 101: Aptamer Fluorophores

Mesothelioma - Causes, Symptoms, and Treatment

Using Video to Share Your Science: We Share Science

Getting the Most from Your Lentiviral Transduction

Antibodies 101: Normalization and Loading Controls for Western Blots

Hot Plasmids: Summer 2024

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Transferable Skills Guide: Conflict Resolution

Hot Biosensors 2022: Year-End Roundup

CRISPR 101: Validating Your Genome Edit

Antibodies 101: Introduction to Gating in Flow Cytometry

An Integrin Antibody Toolkit from IPI

Plasmids 101: Biotinylation

Multiple Plasmids at a Low Price: Kits from Addgene

Site Directed Mutagenesis by PCR

Evolution of Lab Techniques

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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