We narrowed to 400 results for: pho;

1. Plasmids 101: Protein tags

   Type

   Blog Post

   ...tag for purification? Or you want to combine a fluorophore with a tag that localizes your protein to the...

2. Save Time with Transient Plant Leaf Transformations

   Type

   Blog Post

   ...used in C. roseus, but processes specific to photosynthetic active tissues cannot be studied in hairy roots...

3. Designing Your Chalk Talk for the Academic Job Interview

   Type

   Blog Post

   ...distance runner, gardener, cook, and outdoor photographer.  Additional resources on the Addgene blog ...

4. CRISPR 101: Targeting RNA with Cas13a (C2c2)

   Type

   Blog Post

   ...will be cleaved resulting in activation of the fluorophore. The fluorescent signal can be used as an indicator...

5. Hot Plasmids - November 2023

   Type

   Blog Post

   ... promoter. For example, neurons treated with a phorbol ester activator gave fluorescent signal in the ...

6. Antibodies 101: The Four ELISAs and When to Use Them

   Type

   Blog Post

   ... as horseradish peroxidase (HRP) or alkaline phosphatase (AP). Fluorescent tags may also be used, but ...

7. Hot Plasmids Spring 2024

   Type

   Blog Post

   ...6).   Figure 6: Illustration of split fluorophore assay to test reconstitution via mRNA trans-splicing...

8. Plasmids 101: Broad Host Range Plasmids

   Type

   Blog Post

   ...Gluconacetobacter xylinus, Gluconobacter spp., Hyphomicrobium spp., Klebsiella aerogenes, K. pneumoniae, ...

9. Transferable Skills Guide: Career Planning Resources

   Type

   Blog Post

   ...HR, greatly increasing your chances of getting a phone interview! If you don’t have a particular employer...

10. Career Insights: Technical Support Specialist

    Type

    Blog Post

    ...support roles are primarily office based, and email/phone are the most popular ways to communicate with customers...

11. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    ...provided moral support was invaluable.  Mixing metaphors and scientists - Culturing a scientific network...

12. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    ... regulated by guanosine [corrected] 3', 5'-bispyrophosphate: a model for programmed bacterial cell death...

13. 5 Reasons to Use Reddit for Science Communication

    Type

    Blog Post

    ...notes which Evernote even allows me to access on my phone. I use it for everything from receipts to blogs ...

14. Healthcare Consulting: A Door to the Business of Life Sciences

    Type

    Blog Post

    ...chemotherapies, or developing innovative mobile phone applications and services that help diabetic patients...

15. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type

    Blog Post

    ...but rather because of the correct folding and fluorophore formation by most, if not all, of the FP fusions...

16. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    ...440 or 1000bp overlaps to deliver the alkaline phosphatase gene to airway epithelial cells in mice. This...

17. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type

    Blog Post

    ...cleaves a nearby RNA sequence that contains a fluorophore that is quenched when intact and released when...

18. Tips for Getting a Faculty Position

    Type

    Blog Post

    ... my applications all by snail mail and only had phone interviews. We didn't have Skype in 2004. I also...

19. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type

    Blog Post

    ...in several patients developing T-cell acute lymphoblastic leukemia due to insertional mutagenesis (Howe...

20. Transferable Skills Guide: Time Management

    Type

    Blog Post

    ...Probably the easiest way to do this is to leave your phone in your bag on “Do Not Disturb” during the times...

21. 10 Steps to a Perfect Science Talk

    Type

    Blog Post

    ...supposed to be done. They will start looking at their phones. Speaking longer just makes you seem unprepared...

22. Site Directed Mutagenesis by PCR

    Type

    Blog Post

    ...plasmids for the mutation of interest. Gel electrophoresis diagrams of the anticipated restriction analysis...

23. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    ...Khalili K. Elimination of HIV-1 Genomes from Human T-lymphoid Cells by CRISPR/Cas9 Gene Editing. Sci Rep. 2016...

24. How to Negotiate a Successful Lab Start Up Budget

    Type

    Blog Post

    ...possible, this even includes clocks, timers, and phones.  As for reagents and consumables (such as pipet...

25. Finding Your Perfect Job After University

    Type

    Blog Post

    ...help scientists problem solve experiments over the phone or explain how various plasmids work. Most people...

26. Important Considerations When Using AAVs

    Type

    Blog Post

    ...Lung AAV4, AAV5, AAV6, AAV9 Pancreas AAV8 Photoreceptor Cells AAV2, AAV5, AAV8 RPE (Retinal Pigment...

27. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type

    Blog Post

    ...edit. Using Surveyor nuclease followed by gel electrophoresis, these mismatches are indicated by the presence...

28. Sequencing Options for CRISPR Genotyping

    Type

    Blog Post

    ... The results are then visualized using gel electrophoresis. Surveyor™ and T7E1 have been widely adopted...

29. Plasmids 101: Modular Cloning Applications and Kits

    Type

    Blog Post

    .... Many plasmid elements such as promoters or fluorophores are interchangeable across organisms. When using...

30. Selecting Your Plasmid Purification Kit

    Type

    Blog Post

    ...quantity and quality of your plasmid prep is by spectrophotometry. Achieving the highest-quality DNA requires...