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We narrowed to 795 results for: Tec

Showing: 441 - 480 of 795 results

  1. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...experimental process. The majority of current cloning techniques involve the assembly of a circular plasmid in... 1991, Bubeck et al., 1993). But why did this technique fail to gain traction? It is likely that both ...ends on DNA using polymerase chain reaction." Biotechniques 10.1 (1991): 62-66. PubMed PMID: 2003926. 2....

  2. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...key steps to colony PCR are: 1) design primers to detect the presence of your insert; 2) set up a standard...Running a no template control PCR reaction for detecting contamination is also a good idea.    Analyzing....org Read about Choosing a Molecular Cloning Technique Learn to Perform a Bacterial Transformation Deposit...

  3. CRISPR Antimicrobials

    Type
    Blog Post
    ...reduce this likelihood. The key problem with these techniques is efficient delivery, as the antimicrobial must...efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed: PMID 252409288...produce sequence-specific antimicrobials.” Nature Biotechnol. 32(11): 1146-50. (2014). PubMed: PMID 25282355...

  4. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...offer the maximal biosafety available for the technology. The minor limitation in using the 3rd-generation...scientists often refer to as “retroviruses” are technically gamma-retroviruses - another, separate member...transfer vectors for transient transfections? A2: Technically, yes. Will it actually work? That depends. The...

  5. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...expression. Trends in Biotechnology 22:346–353 . https://doi.org/10.1016/j.tibtech.2004.04.006 Hausser ... be used in some in vivo experiments and in biotechnology and therapeutics (Mauro and Chappell 2014).  ...

  6. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...the Dow lab created a base editing reporter that detects and enriches for base editing events in vivo without...conversion) they could induce the translation of any detectable protein, not just fluorescent proteins. ...protein expression, it can be used for an array of detectable proteins including fluorescent proteins, antibiotic...

  7. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...have a natural preference to stay in dark areas (protected) as opposed to brightly illuminated ones (risky...environments by initially moving along a wall (protected) rather than being out in the open (risky). Taking...comprised of a brightly lit (risky) and a dark (protected) area. When measuring the time spent in a particular...

  8. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...A puromycin resistance gene on your vector can protect both plasmid-carrying bacteria and eukaryotes. ...protein synthesis, and the neomycin resistance gene protects against any of them. But different aminoglycosides...host range plasmids shuttle vectors? While it’s technically accurate to say that shuttle vectors have a “...

  9. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...editing in mammalian cells and embryos. Nature Biotechnology, 42(4), 638–650. https://doi.org/10.1038/s41587...with an N-methylpurine DNA glycosylase. Nature Biotechnology, 41(8), 1080–1084. https://doi.org/10.1038/s41587...and C-to-G editing in mammalian cells. Nature Biotechnology, 42(10), 1538–1547. https://doi.org/10.1038/...

  10. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...Leehyo Bioscience 2015 Korea San Diego Biotech Lab 2018 Mexico e-nnovation Life Sciences...Sciences 2020 India Sinapse Biotecnologia 2022 Brazil   In some cases, the distributors broadly...best part of any conference is scientists, from techs to grad students to professors, stopping by to say...

  11. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...the screen. This toxicity was used a proxy for detecting Cas9 cutting. Safe targeting guides better control...off-target cleavage by CRISPR-Cas nucleases." Nature biotechnology 33.2 (2015): 187. Pubmed PMID: 25513782. Pubmed...specificity using truncated guide RNAs." Nature biotechnology 32.3 (2014): 279. Pubmed PMID: 24463574. Pubmed...

  12. Hot Plasmids - November 2023

    Type
    Blog Post
    ...two deposits that increase the utility of this technique.  By engineering a light-sensitive LOV domain ... tapes for intracellular recordings. Nature Biotechnology, 41(5), 631–639. doi: 10.1038/s41587-022-01524... using thick-section and whole-mount labeling techniques. The Trimmer lab’s anti-PSD-95 [K28/43R] recently...

  13. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...integrate the mARGs using PiggyBac. Gas vesicles are detectable under ultrasound and phase contrast microscopy...bioengineering. They are both members of the Shapiro lab at Caltech.   References and Resources References Piraner...monitoring of tumors and tumor-homing bacteria. Nat Biotechnol 1–13 (2023) doi:10.1038/s41587-022-01581-y   ...

  14. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol 33:139–...discovery through the creation of innovative technologies. To date his group has develop several popular...

  15. The Challenges of Cell Culture

    Type
    Blog Post
    ...polymorphisms specific to their origins. This technique is still used today to test for HeLa contamination...test is so good that it is used as a forensic technique (aka DNA fingerprinting) that can place suspects...stance on the matter. Funding bodies such as the Biotechnology and Biological Sciences Research Council (BBSRC...

  16. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...ability of reviewers (and, once published, anyone) to detect possible errors in assumptions, use of inappropriate...senior colleagues. Secondly, science is highly technical and this has a natural, albeit unintentional, ...Policy: Part Two U.S. Office of Science and Technology Policy (OSTP) memo Increasing Access to the ...

  17. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...it comes to altering gene expression, CRISPR technologies have gradually taken over as the preferred method... their promoters in human and mouse. Nature Biotechnology, 35(9), 872–878. https://doi.org/10.1038/nbt... constructing shRNA expression vectors. BMC Biotechnology, 6(1). https://doi.org/10.1186/1472-6750-6-1...

  18. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...keeping. Top priorities in most cases include IP protection, publications and funding. If any of these three...witnessing and audit trails. How does the ELN provider protect and respect your IP rights and data ownership? ...interested in combining scientific research with tech advances. You an follow SciNote on Twitter @scinoteELN...

  19. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...with the contents of the two AAVs. Using this technique, Bok and Porteus deliver a repair template that...respectively. Future researchers could apply this technique to efficiently deliver large repair templates ...AAV vectorization of DSB-mediated gene editing technologies." Current gene therapy 16.3 (2016): 207-219....

  20. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...told all of the nuances of the research in as technical of language as possible. Seriously. My suggested...review of your field or the finer points of your techniques. You DO need to be prepared to know the literature...searches. I've updated chapters to reflect new technologies, i.e. video interviews. For point of reference...

  21. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    .... Alternatively, you can perform simultaneous detection with a mixture of primary antibodies and appropriate..., M., McDonough, L., Zhu, Y., Neumann, E., De Caestecker, M., Gutierrez, D., & Spraggins, J. (2022). Multiplex...Introduction to Antibodies Which Fluorescence Microscopy Technique is Best for Me? Introduction to Immunofluorescence...

  22. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...repair of the break to allow for site-specific detection downstream. The con to these approaches is that...that NHEJ must perform the repair to allow for detection, not Homologous Recombination or Alternative End...effects in CRISPR/Cas9 gene editing. Front Bioeng Biotechnol. 2023 Mar 9;11:1143157. doi: 10.3389/fbioe.2023.1143157...

  23. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...the system, researchers have used split GAL4 architecture or combined the system with Flp/FRT, as reviewed... regulated expression in mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750...expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303-313. doi: 10.1007/s00253...

  24. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ... Why might one be interested in state machine technology? Biological research has produced a massive amount...itself to cheap, quick, and high throughput state detection with flow cytometry. However, expressing reporters...synthetic circuits from genetic context." Nature biotechnology 30.11 (2012): 1137-1142. PubMed PMID: 23034349...

  25. Finding Your Perfect Job After University

    Type
    Blog Post
    ...developing an easy to use diagnostic tool for the detection and diagnosis of different types of pancreatic...trying to get hands-on lab experience is that the technology is so advanced that most of the procedures in...own challenges. I’ve had to become skilled with technology like Skype in order to have meetings between ...

  26. Antibodies 101: Validation

    Type
    Blog Post
    ... good correlation between the levels of target detected in both methods. These approaches have relatively...are ultimately planning to use your antibody to detect endogenous levels of the target in other cells ...Antibody validation: A view from the mountains. New Biotechnol 45:1–8. https://doi.org/10.1016/j.nbt.2018.08.002...

  27. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    .... With the widespread adoption of CRISPR/Cas9 technology and rapid improvements in the field, it has recently...experiments (in collaboration with Integrated DNA Technologies, USA). These synthetic RNAs can also be purchased...delivery of gRNA, Cas9 nuclease, and donor DNA. J Biotechnol 241: 136-146. PubMed PMID: 27845164. 5. Clarke...

  28. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...even if they are present in the sample. Other techniques, like electron microscopy and FRET, can offer...details are still being uncovered, and different techniques all bring their own advantages—and blind spots—to...RNA-programmed Cas9 nuclease specificity. Nature Biotechnology, 31(9), 839–843. https://doi.org/10.1038/nbt...

  29. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    ... looking for the best way to improve your lab techniques — but perhaps are a little limited on time — ...share quick tips to help you master fundamental technical lab skills, like pipetting! We’ll release a new...

  31. CRISPR Guide Update

    Type
    Blog Post
    ...share and describe some incredible new CRISPR technologies developed and widely adopted in the past few...guide for a great introduction to what CRISPR technologies have to offer. Happy CRISPR-ing! More resources...

  32. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    ...software engineers, surprisingly, who have deep technical knowledge but little biology experience.) The ... at MIT. He also writes Codon, a blog about biotechnology.    ...

  34. Call for Guest Bloggers

    Type
    Blog Post
    ...recent advances in the biological sciences and techniques that simplify and expedite research. We recognize...through our blog. Do you have skill in a particular technique? Do you have a set of tips you give to every new...

  35. Summer Fun at Addgene!

    Type
    Blog Post
    ... with our 101 series, which introduces common techniques and concepts. But what if you’re looking for ...Engineered Systemic AAVs” with the director of CalTech’s CLOVER lab, Tim Miles, PhD, we invite you to head...

  36. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...podcast that introduces you to 4 new plasmid technologies from one of our recent newsletters. Listen ...you enjoy this new way to learn about plasmid technologies. If you have any comments about the Hot Plasmids...

  38. Editor's Choice, October 2016

    Type
    Blog Post
    ...ways to use CRISPR in plants, to learn techniques for detecting genome edits, to discover why you might...

  39. Editor's Choice, July 2016

    Type
    Blog Post
    ...views) was an update on new CRISPR genome editing technology, Cpf1. Written by our Science Communications ...the end of the post also really helped put this technology into a concrete context. I recommend this post...
Showing: 441 - 480 of 795 results