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We narrowed to 916 results for: Tro;

Showing: 551 - 600 of 916 results

  1. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...impossible for a career scientist such as Zimmer to introduce any one of these topics without some editorializing...fellow scientists--but that doesn’t make his introduction to these topics any less effective. Who is this...non-scientists in your life, the book may be the introduction they need to grasp the challenges you face in...

  2. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...standpoint, a plasmid with all four genes and necessary control elements for each would be relatively large. Cloning...separate plasmids allows researchers to partially control expression levels of each gene by altering the ...genes (either viral genes or minigenome) are under control of a T7 RNA polymerase promoter sequence. Thus,...

  3. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...comment, annotate, and edit protocols. After my introduction to protocols.io, I decided to research what ...orders, tracking students and research projects, electronic lab notebooks and protocol management. Though...More Reading: How to manage scientific data: Electronic lab notebooks compared with Google Docs and wikis...

  4. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... for saturation mutagenesis, and they provide a strong proof of concept for further development. ...excision repair pathway. The team behind AYBEv3 introduced the first deaminase-free base editor when they... called gTBE and gCBE, with Addgene. gTBE can introduce T → C and T → G edits, though with limited selectivity...

  5. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...degraders. But CRBN has been difficult to study in vitro, requiring either expressing it with additional ...codon-optimized for C. elegans and included artificial introns to improve expression. When using these constructs... an edited strain carrying Cas9 to allow the introduction of additional sgRNAs. Image reused from Neo ...

  6. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...a large number of requests for vectors in the “controls” category, which includes things like viral vectors...vectors in a variety of serotypes, including the retrograde AAVrg and the systemic capsid PHP.eB.   ...Patrick, M., Guerin, K., & Fan, M. (2023). Quality control for Adeno-associated viral vector production. Neuromethods...

  7. Bioinformatics at Addgene

    Type
    Blog Post
    ...laboratories and associated publications, but our quality control (QC) standards have evolved over the years to keep... ability to re-use or borrow existing code and control how the various pieces were connected, as this ... for Bioinformatics AnalysesAAV Vector Quality Control: Going the Extra Mile with NGS...

  8. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...provided a major toehold into understanding how genes control biology (a fact celebrated by the Nobel prize committee...transgenics Creating transgenic organisms by introducing foreign DNA into the genome is useful for a variety...into adults and explain how signaling pathways control this process. Additional zebrafish applications...

  9. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...of symptoms in animal models, Rett syndrome is a strong translational candidate for gene replacement therapy...therapy. In 2014, RSRT brought together a strong collaboration between two gene therapy labs (Brian Kaspar...neurological disease (Sinnamon et al., 2020). They introduced a mouse MECP2 guide RNA and a hyperactive version...

  10. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...advantages. An introduction to Gateway technology The Gateway cloning method, developed by Invitrogen, is an ...an in vitro version of the integration and excision recombination reactions that take place when lambda...

  11. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...protein instead localizes to the cytoplasm? Or more troubling, what if a significant fraction of the fusion ...these compartments using CRISPR systems should strongly consider using moxFPs over standard FPs for the...Structural Biology. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

  12. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...epigenetic silencer Recombinant antibodies for marking astrocytes MagIC-Cryo-EM for sample enrichment and structure...determination By Mike Lacy Conventional cryogenic electron microscopy (cryo-EM) protocols typically require...glia-specific intermediate filament expressed in astrocytes throughout the brain, making it a popular target...

  13. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde) pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde) pGP-AAV-syn-FLEX-jGCaMP8m-WPRE...have Kd around 70 mM and 100 mM, respectively. Introducing mutations and using potassium-binding protein...

  15. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...transcriptional regulation, as long as they can control recombinase activity. For example, post-transcriptional...methods section of our paper (1). How might one troubleshoot GSRMs that don't work as expected? The successful...the parts used to build them. We recommend using strong, directional terminators and promoters with buffer...

  16. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...the number of integration copies can be tightly controlled by titrating the transposase to transposon ratios... levels of your gene of interest, as needed. Controlling the expression levels of your gene may be particularly...splinkerette-PCR method for the isolation and sequencing of retroviral insertion sites.,” Nat. Protoc., vol. 4, no. ...

  17. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...interaction is not covalent, later work showed that introducing a Leu28Cys mutation in eDHFR does lead to covalent...2). If you’re working with purified proteins in vitro, you can even use the same tag for multiple proteins... actin filament assembly-regulating proteins in vitro reveals complex events. a) Micrographs of a fluorescently-labeled...

  18. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...CRISPR/Cas9 allows us to delete sequences and introduce mutations and epitopes with unprecedented ease...in 1-2 kilobases of additional sequence being introduced. Cre-mediated excision of the selective cassette... ML, Jorgensen EM (2018) SapTrap vectors for introducing point mutations with unc-119+ selection. https...

  19. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...and LIIbeta F 1-2 RNAi, which allows assembly of intron-spliced hpRNAs through Golden Gate cloning. Artificial...Identifying actively silenced plants Once you’ve introduced your RNAi into your organism, how do you identify...insertion of any target sequence, as well as essential control plasmids. Many thanks to our guest blogger Robert...

  20. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...happy cloning! This article was written by Susanna Stroik and Meghan Rego, with thanks to Rob Hurt for his...Plants Mediated by a Cas9 Gene Containing Multiple Introns.” Plant Communications, vol. 2, no. 2, Elsevier...Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells. Cold Spring Harbor ...

  21. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...substance (fluorophore) that has absorbed light or electromagnetic radiation at another wavelength (Heinrichs,...Heinrichs, 2009). When a fluorophore absorbs light, electrons are elevated from a ground state to an excited state...After a brief elevation in this excited state, the electron returns to its ground state by emitting a photon...

  24. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español! Esta traducción... aumentar el acceso global a los materiales de nuestro repositorio. Este increíble regalo permitirá que...

  27. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...not just for a better day at the office but for stronger science as well. This blog is our way of continuing...Principal Investigator. How-to guides for troubleshooting your experiments and getting better results...

  28. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    ...here! Podcast Breakdown (Time: Topic) 0:00-1:00 : Intro 1:01-3:48 : Kingdom Kwapata's background and education... : Kingdom's Thoughts on his time in Boston and Outro We'd like to thank Dr. Kwapata for taking the time...

  30. CRISPR Cheat Sheet

    Type
    Blog Post
    ...bacteria that allow the bacteria to target and destroy viruses that infect them. Commonly referred to ...With a repair template, CRISPR can be used to introduce a functional change in the DNA sequence. Base ...

  31. Summer Fun at Addgene!

    Type
    Blog Post
    ...tricks for sharing your science with others, and introducing science communication as a research field. And...probably are familiar with our 101 series, which introduces common techniques and concepts. But what if you...

  32. Our New Antibody Mascot is....

    Type
    Blog Post
    ...some brand-new Addgene swag!   Last month, we introduced you to Blugene and Aavery's newest friend, our...Aavery would like your attention, please, as they introduce their new friend…  Fig. 1: Meet...well,...

  37. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    .... Not all of these technologies were without controversy, and it remains to be seen how popular any one...our most requested plasmid, SpCas9 is still going strong as the basis for many genome editing experiments...

  38. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...the general public that provides an accessible introduction to various scientific topics. Over twenty graduate...the audience engaged. The day kicked off with introductory lectures targeting three broad concepts: the...

  43. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...at eight different states of assembly from cryo-electron microscopy, the team reveal the mechanism behind...genome editing by fusing Cas9 to a mutated human estrogen receptor. This protein fusion stays in the cytoplasm...

  44. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...through any search, ordering, technical, or quality control issues. Improving customer support with self service...re looking for it, or you may just prefer to troubleshoot yourself without contacting someone else and...

  45. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    .... The kanamycin antibiotic resistance gene is controlled by a weaker constitutive Nos promoter, and it...Transgenesis with GreenGate Plasmids Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More Plasmids...

  46. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...worth it. Read here to get help if you are having trouble preparing your most excellent resume. 2) Follow... the criteria. Note the criteria that you meet strongly and note the ones that you could meet with learning...

  48. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ... sure to include various positive and negative controls to “convince [any] old skeptic professor who's...conference is a big multidisciplinary one.” Cepko strongly emphasizes that people should “follow their passions...

  49. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ... light in the near-infrared portion of the electromagnetic spectrum – the spectral region in which mammalian...Nature Communications last year, “The capability to control spectral properties of PA FPs with light of specific...

  50. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...You’ve designed the perfect experiment – controls, conditions, and everything in between – now all you... Susanna Stroik...
Showing: 551 - 600 of 916 results