We narrowed to 911 results for: Tro;

...protein instead localizes to the cytoplasm? Or more troubling, what if a significant fraction of the fusion ...these compartments using CRISPR systems should strongly consider using moxFPs over standard FPs for the...Structural Biology. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

...epigenetic silencer Recombinant antibodies for marking astrocytes MagIC-Cryo-EM for sample enrichment and structure...determination By Mike Lacy Conventional cryogenic electron microscopy (cryo-EM) protocols typically require...glia-specific intermediate filament expressed in astrocytes throughout the brain, making it a popular target...

...pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde) pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde) pGP-AAV-syn-FLEX-jGCaMP8m-WPRE...have Kd around 70 mM and 100 mM, respectively. Introducing mutations and using potassium-binding protein...

...handed out at the event). Program 0 - 1:24 - Intro 1:24 - 29:31 - Reproducibility Overview - Jeffrey... Addgene as a source of sequenced and quality controlled plasmids and viral vectors. When working with... easier to learn new experimental techniques. Electronic lab notebooks also play a role here by making...

...transcriptional regulation, as long as they can control recombinase activity. For example, post-transcriptional...methods section of our paper (1). How might one troubleshoot GSRMs that don't work as expected? The successful...the parts used to build them. We recommend using strong, directional terminators and promoters with buffer...

...the number of integration copies can be tightly controlled by titrating the transposase to transposon ratios... levels of your gene of interest, as needed. Controlling the expression levels of your gene may be particularly...splinkerette-PCR method for the isolation and sequencing of retroviral insertion sites.,” Nat. Protoc., vol. 4, no. ...

...and LIIbeta F 1-2 RNAi, which allows assembly of intron-spliced hpRNAs through Golden Gate cloning. Artificial...Identifying actively silenced plants Once you’ve introduced your RNAi into your organism, how do you identify...insertion of any target sequence, as well as essential control plasmids. Many thanks to our guest blogger Robert...

...CRISPR/Cas9 allows us to delete sequences and introduce mutations and epitopes with unprecedented ease...in 1-2 kilobases of additional sequence being introduced. Cre-mediated excision of the selective cassette... ML, Jorgensen EM (2018) SapTrap vectors for introducing point mutations with unc-119+ selection. https...

...happy cloning! This article was written by Susanna Stroik and Meghan Rego, with thanks to Rob Hurt for his...Plants Mediated by a Cas9 Gene Containing Multiple Introns.” Plant Communications, vol. 2, no. 2, Elsevier...Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells. Cold Spring Harbor ...

...interaction is not covalent, later work showed that introducing a Leu28Cys mutation in eDHFR does lead to covalent...2). If you’re working with purified proteins in vitro, you can even use the same tag for multiple proteins... actin filament assembly-regulating proteins in vitro reveals complex events. a) Micrographs of a fluorescently-labeled...

...substance (fluorophore) that has absorbed light or electromagnetic radiation at another wavelength (Heinrichs,...Heinrichs, 2009). When a fluorophore absorbs light, electrons are elevated from a ground state to an excited state...After a brief elevation in this excited state, the electron returns to its ground state by emitting a photon...

...representative of the communities’ opinion. The trouble is, with so much choice, in those few moments after...second major area deals in post-experiment quality control, a good example is CRISPR-GA. These tools assess...place to focus further development toward. Quality control is, however, not a focus of this blog post. I separate...

...series, you'll learn about optogenetics tools for controlling protein activity, methods for increasing CRISPR...Plasmid 1: Photocleaveable PhoCl protein for controlling molecular processes with light. Listen to Our...

...¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español! Esta traducción... aumentar el acceso global a los materiales de nuestro repositorio. Este increíble regalo permitirá que...

...interview with Wei Leong Chew! Podcast breakdown Intro: Wei Leong Chew's career path and why he chose to...gene delivery methods, AAVs, and more. Chapter 7/Outro: Advice for researchers pursuing careers in academia...

...interview with Joachim Goedhart Podcast breakdown Intro Chapter 1 - How Joachim ended up at the University...other nontraditional methods including science art. Outro Many thanks to our guest, Joachim Goεdhart! Joachim...

...not just for a better day at the office but for stronger science as well. This blog is our way of continuing...Principal Investigator. How-to guides for troubleshooting your experiments and getting better results...

...here! Podcast Breakdown (Time: Topic) 0:00-1:00 : Intro 1:01-3:48 : Kingdom Kwapata's background and education... : Kingdom's Thoughts on his time in Boston and Outro We'd like to thank Dr. Kwapata for taking the time...

...the interview with Crystal Shih Podcast breakdown Intro Chapter 1: Who is Crystal Shih? Crystal's educational...importance of mentorship in developing career skills. Outro Many thanks to our guest, Crystal Shih. Crystal...

...tricks for sharing your science with others, and introducing science communication as a research field. And...probably are familiar with our 101 series, which introduces common techniques and concepts. But what if you...

...some brand-new Addgene swag!   Last month, we introduced you to Blugene and Aavery's newest friend, our...Aavery would like your attention, please, as they introduce their new friend…  Fig. 1: Meet...well,...

...bacteria that allow the bacteria to target and destroy viruses that infect them. Commonly referred to ...With a repair template, CRISPR can be used to introduce a functional change in the DNA sequence. Base ...

...preparation is quality controlled through next generation sequencing and spectrophotometry to test purity (260...

... the interview with Tom Ellis Podcast breakdown Intro: How Tom ended up in academia and his research goals...research go faster through Open Access. Chapter 7/Outro: Tom’s Involvement with iGEM and Addgene  Many ...

...: Topic 0:00 - 0:50 : Intro 0:51 - 3:08 : The importance of developing a strong graduate student community...

...Plasmid Plasmids 101: Control Plasmids 6 Tips for Analyzing and Troubleshooting DNA Sequencing Results...

.... Not all of these technologies were without controversy, and it remains to be seen how popular any one...our most requested plasmid, SpCas9 is still going strong as the basis for many genome editing experiments...

...the general public that provides an accessible introduction to various scientific topics. Over twenty graduate...the audience engaged. The day kicked off with introductory lectures targeting three broad concepts: the...

...ways that I could change the world around me. My strong interest in the intersections of media, art, and...content that was unique but also brand friendly. I introduced the team to a new design program, Canva, and ...

...success. Sometimes, you might have a few positive control genes on which to test the validity of your biological... the whole point is that there are no positive controls for this phenotype – you’ve developed a new model...

... their favorites, and trophies are awarded and coveted. I’ve only won 2 trophies over the years, which...

...Concept These systems were benchmarked on positive control genes in CD4+ and CD8+ T cells. To demonstrate ... W., Nguyen, T. H., Coxe, M. A., Yates, K. B., Trombley, J. D., Weiss, S. A., Brown, F. D., Gillis, J....

...at eight different states of assembly from cryo-electron microscopy, the team reveal the mechanism behind...genome editing by fusing Cas9 to a mutated human estrogen receptor. This protein fusion stays in the cytoplasm...

...through any search, ordering, technical, or quality control issues. Improving customer support with self service...re looking for it, or you may just prefer to troubleshoot yourself without contacting someone else and...

.... The kanamycin antibiotic resistance gene is controlled by a weaker constitutive Nos promoter, and it...Transgenesis with GreenGate Plasmids Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More Plasmids...

...worth it. Read here to get help if you are having trouble preparing your most excellent resume. 2) Follow... the criteria. Note the criteria that you meet strongly and note the ones that you could meet with learning...

...interested in meeting movie stars, but when I met the astronaut Jim Lovell I was speechless (the club of people...students in their labs and the importance of the strong, cross-disciplinary collaboration they shared to...

... sure to include various positive and negative controls to “convince [any] old skeptic professor who's...conference is a big multidisciplinary one.” Cepko strongly emphasizes that people should “follow their passions...

... light in the near-infrared portion of the electromagnetic spectrum – the spectral region in which mammalian...Nature Communications last year, “The capability to control spectral properties of PA FPs with light of specific...

...Each plasmid page now has a helpful graphic to introduce you to the plasmid class covered on that page....Using CRISPR/Cas9 and Plasmids 101: NGS Quality Control for Pooled Libraries Day 12: New website resources...

...policy gives us time to complete necessary quality control and legal approvals so the plasmids are ready for...start out with this hold! You will always have control over when your plasmids are distributed. Next month...

...researchers in developing countries. We are proud to introduce the 5 scientists who were awarded the Addgene-...technology to engineer plants that are able to destroy DNA viruses, similar to the anti-phage CRISPR effect...

...a reporter signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent... being especially popular. We’ll give you an introduction to fluorescent biosensors, but keep in mind ...

...You’ve designed the perfect experiment – controls, conditions, and everything in between – now all you... Susanna Stroik...

...   Listen this episode of Hot Plasmids! Light controls the activity of a microtubule-associated protein...studying cellular dynamics. The photoinducible control of protein function is more specific and much faster...

...surprised to see that it would open not with an introduction to CRISPR nucleases, but with a session on DNA...editing rates across multiple systems. Read our introductory blog post, then check out this RNP protocol ...

...endogenous IgGs, you should include no primary antibody controls in your experiments. If you do find high levels...resources on the Addgene blog Antibodies 101: Introduction to Immunofluorescence Antibodies 101: Affinity...

...Culture Adenoviral Vector Production and Troubleshooting Additional resources on Addgene.org AAV ...  Susanna Stroik...

...packed with tech transfer resources (summaries, electronic MTA approval, archive of old MTAs, and more) ... Transfer Information Tech Transfer News: A Reintroduction to the UBMTA ...

...cell types. MEFs were reprogrammed into iEPs by retroviral expression of transcription factors Foxa1 and.... Adding Mettl7a1 to the standard Foxa1-Hnf4α retroviral overexpression cocktail yielded a three-fold ...